SSD1 suppresses phenotypes induced by the lack of Elongator-dependent tRNA modifications
(2019) In PLoS Genetics 15(8).- Abstract
The Elongator complex promotes formation of 5-methoxycarbonylmethyl (mcm5) and 5-carbamoylmethyl (ncm5) side-chains on uridines at the wobble position of cytosolic eukaryotic tRNAs. In all eukaryotic organisms tested to date, the inactivation of Elongator not only leads to the lack of mcm5/ncm5 groups in tRNAs, but also a wide variety of additional phenotypes. Although the phenotypes are most likely caused by a translational defect induced by reduced functionality of the hypomodified tRNAs, the mechanism(s) underlying individual phenotypes are poorly understood. In this study, we show that the genetic background modulates the phenotypes induced by the lack of mcm5/ncm5 groups in Saccharomyces cerevisiae. We show that the stress-induced... (More)
The Elongator complex promotes formation of 5-methoxycarbonylmethyl (mcm5) and 5-carbamoylmethyl (ncm5) side-chains on uridines at the wobble position of cytosolic eukaryotic tRNAs. In all eukaryotic organisms tested to date, the inactivation of Elongator not only leads to the lack of mcm5/ncm5 groups in tRNAs, but also a wide variety of additional phenotypes. Although the phenotypes are most likely caused by a translational defect induced by reduced functionality of the hypomodified tRNAs, the mechanism(s) underlying individual phenotypes are poorly understood. In this study, we show that the genetic background modulates the phenotypes induced by the lack of mcm5/ncm5 groups in Saccharomyces cerevisiae. We show that the stress-induced growth defects of Elongator mutants are stronger in the W303 than in the closely related S288C genetic background and that the phenotypic differences are caused by the known polymorphism at the locus for the mRNA binding protein Ssd1. Moreover, the mutant ssd1 allele found in W303 cells is required for the reported histone H3 acetylation and telomeric gene silencing defects of Elongator mutants. The difference at the SSD1 locus also partially explains why the simultaneous lack of mcm5 and 2-thio groups at wobble uridines is lethal in the W303 but not in the S288C background. Collectively, our results demonstrate that the SSD1 locus modulates phenotypes induced by the lack of Elongator-dependent tRNA modifications.
(Less)
- author
- Xu, Fu ; Byström, Anders S and Johansson, Marcus J O LU
- publishing date
- 2019-08
- type
- Contribution to journal
- publication status
- published
- keywords
- Gene Expression/genetics, Genotype, Histone Acetyltransferases/genetics, Peptide Elongation Factors/genetics, Phenotype, RNA Processing, Post-Transcriptional/genetics, RNA, Transfer/genetics, RNA-Binding Proteins/metabolism, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae Proteins/genetics, Uridine/analogs & derivatives
- in
- PLoS Genetics
- volume
- 15
- issue
- 8
- article number
- e1008117
- publisher
- Public Library of Science (PLoS)
- external identifiers
-
- scopus:85072133857
- pmid:31465447
- ISSN
- 1553-7404
- DOI
- 10.1371/journal.pgen.1008117
- language
- English
- LU publication?
- no
- id
- a765acb0-e6f6-4352-a3ba-14b42109929c
- date added to LUP
- 2024-02-28 17:36:10
- date last changed
- 2024-04-14 00:23:07
@article{a765acb0-e6f6-4352-a3ba-14b42109929c,
abstract = {{<p>The Elongator complex promotes formation of 5-methoxycarbonylmethyl (mcm5) and 5-carbamoylmethyl (ncm5) side-chains on uridines at the wobble position of cytosolic eukaryotic tRNAs. In all eukaryotic organisms tested to date, the inactivation of Elongator not only leads to the lack of mcm5/ncm5 groups in tRNAs, but also a wide variety of additional phenotypes. Although the phenotypes are most likely caused by a translational defect induced by reduced functionality of the hypomodified tRNAs, the mechanism(s) underlying individual phenotypes are poorly understood. In this study, we show that the genetic background modulates the phenotypes induced by the lack of mcm5/ncm5 groups in Saccharomyces cerevisiae. We show that the stress-induced growth defects of Elongator mutants are stronger in the W303 than in the closely related S288C genetic background and that the phenotypic differences are caused by the known polymorphism at the locus for the mRNA binding protein Ssd1. Moreover, the mutant ssd1 allele found in W303 cells is required for the reported histone H3 acetylation and telomeric gene silencing defects of Elongator mutants. The difference at the SSD1 locus also partially explains why the simultaneous lack of mcm5 and 2-thio groups at wobble uridines is lethal in the W303 but not in the S288C background. Collectively, our results demonstrate that the SSD1 locus modulates phenotypes induced by the lack of Elongator-dependent tRNA modifications.</p>}},
author = {{Xu, Fu and Byström, Anders S and Johansson, Marcus J O}},
issn = {{1553-7404}},
keywords = {{Gene Expression/genetics; Genotype; Histone Acetyltransferases/genetics; Peptide Elongation Factors/genetics; Phenotype; RNA Processing, Post-Transcriptional/genetics; RNA, Transfer/genetics; RNA-Binding Proteins/metabolism; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins/genetics; Uridine/analogs & derivatives}},
language = {{eng}},
number = {{8}},
publisher = {{Public Library of Science (PLoS)}},
series = {{PLoS Genetics}},
title = {{SSD1 suppresses phenotypes induced by the lack of Elongator-dependent tRNA modifications}},
url = {{http://dx.doi.org/10.1371/journal.pgen.1008117}},
doi = {{10.1371/journal.pgen.1008117}},
volume = {{15}},
year = {{2019}},
}