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Solid-liquid interface method (SLIM) : a new crystallization method for proteins

Brostromer, Erik ; Nan, Jie LU ; Li, Lan-Fen and Su, Xiao-Dong LU (2009) In Biochemical and Biophysical Research Communications 386(4). p.8-634
Abstract

Despite impressive advances in theories, methods and technologies, crystallization still remains a serious bottleneck in structural determination of macromolecules. Here we present a novel solid-liquid interface method (SLIM) for protein crystallization, based on the pre-adding and drying of a crystallization reagent, and thereafter the dispensing of a protein solution to the dried media to initiate crystallization from the solid-liquid interface. Not only quick and easy to perform, the method also allows for a less concentrated protein solution for setting up crystallization trials.

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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
keywords
Crystallization, Proteins
in
Biochemical and Biophysical Research Communications
volume
386
issue
4
pages
5 pages
publisher
Elsevier
external identifiers
  • pmid:19545537
  • scopus:67650451389
ISSN
1090-2104
DOI
10.1016/j.bbrc.2009.06.086
language
English
LU publication?
yes
id
a792aef0-e812-4901-8e38-d2311a853066
date added to LUP
2016-09-07 22:52:39
date last changed
2023-01-06 07:48:21
@article{a792aef0-e812-4901-8e38-d2311a853066,
  abstract     = {{<p>Despite impressive advances in theories, methods and technologies, crystallization still remains a serious bottleneck in structural determination of macromolecules. Here we present a novel solid-liquid interface method (SLIM) for protein crystallization, based on the pre-adding and drying of a crystallization reagent, and thereafter the dispensing of a protein solution to the dried media to initiate crystallization from the solid-liquid interface. Not only quick and easy to perform, the method also allows for a less concentrated protein solution for setting up crystallization trials.</p>}},
  author       = {{Brostromer, Erik and Nan, Jie and Li, Lan-Fen and Su, Xiao-Dong}},
  issn         = {{1090-2104}},
  keywords     = {{Crystallization; Proteins}},
  language     = {{eng}},
  month        = {{09}},
  number       = {{4}},
  pages        = {{8--634}},
  publisher    = {{Elsevier}},
  series       = {{Biochemical and Biophysical Research Communications}},
  title        = {{Solid-liquid interface method (SLIM) : a new crystallization method for proteins}},
  url          = {{http://dx.doi.org/10.1016/j.bbrc.2009.06.086}},
  doi          = {{10.1016/j.bbrc.2009.06.086}},
  volume       = {{386}},
  year         = {{2009}},
}