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Solid-liquid interface method (SLIM) : a new crystallization method for proteins

Brostromer, Erik; Nan, Jie LU ; Li, Lan-Fen and Su, Xiao-Dong LU (2009) In Biochemical and Biophysical Research Communications 386(4). p.8-634
Abstract

Despite impressive advances in theories, methods and technologies, crystallization still remains a serious bottleneck in structural determination of macromolecules. Here we present a novel solid-liquid interface method (SLIM) for protein crystallization, based on the pre-adding and drying of a crystallization reagent, and thereafter the dispensing of a protein solution to the dried media to initiate crystallization from the solid-liquid interface. Not only quick and easy to perform, the method also allows for a less concentrated protein solution for setting up crystallization trials.

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author
organization
publishing date
type
Contribution to journal
publication status
published
keywords
Crystallization, Proteins
in
Biochemical and Biophysical Research Communications
volume
386
issue
4
pages
5 pages
publisher
Elsevier
external identifiers
  • scopus:67650451389
ISSN
1090-2104
DOI
10.1016/j.bbrc.2009.06.086
language
English
LU publication?
yes
id
a792aef0-e812-4901-8e38-d2311a853066
date added to LUP
2016-09-07 22:52:39
date last changed
2017-01-01 08:33:31
@article{a792aef0-e812-4901-8e38-d2311a853066,
  abstract     = {<p>Despite impressive advances in theories, methods and technologies, crystallization still remains a serious bottleneck in structural determination of macromolecules. Here we present a novel solid-liquid interface method (SLIM) for protein crystallization, based on the pre-adding and drying of a crystallization reagent, and thereafter the dispensing of a protein solution to the dried media to initiate crystallization from the solid-liquid interface. Not only quick and easy to perform, the method also allows for a less concentrated protein solution for setting up crystallization trials.</p>},
  author       = {Brostromer, Erik and Nan, Jie and Li, Lan-Fen and Su, Xiao-Dong},
  issn         = {1090-2104},
  keyword      = {Crystallization,Proteins},
  language     = {eng},
  month        = {09},
  number       = {4},
  pages        = {8--634},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {Solid-liquid interface method (SLIM) : a new crystallization method for proteins},
  url          = {http://dx.doi.org/10.1016/j.bbrc.2009.06.086},
  volume       = {386},
  year         = {2009},
}