Glucose transport activity measured in giant vesicles
(2018) In Methods in Molecular Biology 1713. p.77-91- Abstract
Incorporation of membrane proteins and internal reporter systems directly into giant vesicles, during their formation from a hydrogel surface, has emerged as a promising new concept in membrane protein characterization. Here, we provide the detailed protocol for a glucose transporter activity assay based on giant vesicles containing a fluorescent enzyme-linked reporter system internally. This assay is applicable for the functional analysis of a variety of hexose-transporting proteins. We furthermore believe that it can aid in the development of drugs targeting hexose transporters.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/a7a97393-1746-4f5e-8595-1afa32b45e60
- author
- Hansen, Jesper S. LU and Lindkvist-Petersson, Karin LU
- organization
- publishing date
- 2018
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Activity assay, Fluorescence microscopy, Giant vesicles, Glucose transport, Glucose transporters, GLUT1, Membrane protein, Resorufin
- host publication
- Methods in Molecular Biology
- series title
- Methods in Molecular Biology
- volume
- 1713
- pages
- 15 pages
- publisher
- Humana Press
- external identifiers
-
- pmid:29218519
- scopus:85037597742
- ISSN
- 1064-3745
- DOI
- 10.1007/978-1-4939-7507-5_7
- language
- English
- LU publication?
- yes
- id
- a7a97393-1746-4f5e-8595-1afa32b45e60
- date added to LUP
- 2017-12-21 08:34:15
- date last changed
- 2024-03-01 03:23:37
@inbook{a7a97393-1746-4f5e-8595-1afa32b45e60,
abstract = {{<p>Incorporation of membrane proteins and internal reporter systems directly into giant vesicles, during their formation from a hydrogel surface, has emerged as a promising new concept in membrane protein characterization. Here, we provide the detailed protocol for a glucose transporter activity assay based on giant vesicles containing a fluorescent enzyme-linked reporter system internally. This assay is applicable for the functional analysis of a variety of hexose-transporting proteins. We furthermore believe that it can aid in the development of drugs targeting hexose transporters.</p>}},
author = {{Hansen, Jesper S. and Lindkvist-Petersson, Karin}},
booktitle = {{Methods in Molecular Biology}},
issn = {{1064-3745}},
keywords = {{Activity assay; Fluorescence microscopy; Giant vesicles; Glucose transport; Glucose transporters; GLUT1; Membrane protein; Resorufin}},
language = {{eng}},
pages = {{77--91}},
publisher = {{Humana Press}},
series = {{Methods in Molecular Biology}},
title = {{Glucose transport activity measured in giant vesicles}},
url = {{http://dx.doi.org/10.1007/978-1-4939-7507-5_7}},
doi = {{10.1007/978-1-4939-7507-5_7}},
volume = {{1713}},
year = {{2018}},
}