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Glucose transport activity measured in giant vesicles

Hansen, Jesper S. LU and Lindkvist-Petersson, Karin LU (2018) In Methods in Molecular Biology 1713. p.77-91
Abstract

Incorporation of membrane proteins and internal reporter systems directly into giant vesicles, during their formation from a hydrogel surface, has emerged as a promising new concept in membrane protein characterization. Here, we provide the detailed protocol for a glucose transporter activity assay based on giant vesicles containing a fluorescent enzyme-linked reporter system internally. This assay is applicable for the functional analysis of a variety of hexose-transporting proteins. We furthermore believe that it can aid in the development of drugs targeting hexose transporters.

Please use this url to cite or link to this publication:
author
and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Activity assay, Fluorescence microscopy, Giant vesicles, Glucose transport, Glucose transporters, GLUT1, Membrane protein, Resorufin
host publication
Methods in Molecular Biology
series title
Methods in Molecular Biology
volume
1713
pages
15 pages
publisher
Humana Press
external identifiers
  • pmid:29218519
  • scopus:85037597742
ISSN
1064-3745
DOI
10.1007/978-1-4939-7507-5_7
language
English
LU publication?
yes
id
a7a97393-1746-4f5e-8595-1afa32b45e60
date added to LUP
2017-12-21 08:34:15
date last changed
2021-08-18 01:51:48
@inbook{a7a97393-1746-4f5e-8595-1afa32b45e60,
  abstract     = {<p>Incorporation of membrane proteins and internal reporter systems directly into giant vesicles, during their formation from a hydrogel surface, has emerged as a promising new concept in membrane protein characterization. Here, we provide the detailed protocol for a glucose transporter activity assay based on giant vesicles containing a fluorescent enzyme-linked reporter system internally. This assay is applicable for the functional analysis of a variety of hexose-transporting proteins. We furthermore believe that it can aid in the development of drugs targeting hexose transporters.</p>},
  author       = {Hansen, Jesper S. and Lindkvist-Petersson, Karin},
  booktitle    = {Methods in Molecular Biology},
  issn         = {1064-3745},
  language     = {eng},
  pages        = {77--91},
  publisher    = {Humana Press},
  series       = {Methods in Molecular Biology},
  title        = {Glucose transport activity measured in giant vesicles},
  url          = {http://dx.doi.org/10.1007/978-1-4939-7507-5_7},
  doi          = {10.1007/978-1-4939-7507-5_7},
  volume       = {1713},
  year         = {2018},
}