Advanced

Purification and partial sequence analysis of the soluble catechol-o-methyltransferase from human placenta : Comparison to the rat liver enzyme

Tilgmann, Carola LU and Kalkkinen, Nisse (1991) In Biochemical and Biophysical Research Communications 174(2). p.995-1002
Abstract

Catechol-o-methyltransferase from human placenta was purified 1400-fold by hydroxyapatite adsorption, ammonium sulfate precipitation, gel filtration, high performance anion- exchange and reversed-phase chromatography. The purified enzyme has an apparent molecular weight of 26.000, an isoelectric point of 5,3 and is activated ten-fold in the presence of 20mM cysteine. The enzyme shows primary structure homology to the corresponding rat liver soluble enzyme, based on the sequenced tryptic peptides.

Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
in
Biochemical and Biophysical Research Communications
volume
174
issue
2
pages
8 pages
publisher
Elsevier
external identifiers
  • scopus:0025978802
ISSN
0006-291X
DOI
10.1016/0006-291X(91)91517-G
language
English
LU publication?
no
id
a7f41c5e-3775-467d-bf38-3d2560470f90
date added to LUP
2016-04-11 13:25:49
date last changed
2017-07-30 05:09:27
@article{a7f41c5e-3775-467d-bf38-3d2560470f90,
  abstract     = {<p>Catechol-o-methyltransferase from human placenta was purified 1400-fold by hydroxyapatite adsorption, ammonium sulfate precipitation, gel filtration, high performance anion- exchange and reversed-phase chromatography. The purified enzyme has an apparent molecular weight of 26.000, an isoelectric point of 5,3 and is activated ten-fold in the presence of 20mM cysteine. The enzyme shows primary structure homology to the corresponding rat liver soluble enzyme, based on the sequenced tryptic peptides.</p>},
  author       = {Tilgmann, Carola and Kalkkinen, Nisse},
  issn         = {0006-291X},
  language     = {eng},
  month        = {01},
  number       = {2},
  pages        = {995--1002},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {Purification and partial sequence analysis of the soluble catechol-o-methyltransferase from human placenta : Comparison to the rat liver enzyme},
  url          = {http://dx.doi.org/10.1016/0006-291X(91)91517-G},
  volume       = {174},
  year         = {1991},
}