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Trypsin activation of porcine procolipase. Kinetics of activation and effects on lipid binding

Wieloch, Tadeusz LU (1985) In FEBS Letters 185(1). p.63-66
Abstract

The kinetics of trypsin activation of pancreatic procolipase was investigated and the pH dependence of the binding of procolipase and colipase to a tributyrine-bile salt interface studied. The Km was 0.06 mM and Kcat 8 s-1, and was of the same order of magnitude as for the activation of pancreatic zymogens. At basic pH values colipase had a higher affinity for the tributyrine-bile salt interface as compared to procolipase. The trypsin activation of procolipase ensures a rapid degradation of dietary lipids in the intestine.

Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Colipase, Kinetics, Lipid binding, Procolipase, Trypsin activation
in
FEBS Letters
volume
185
issue
1
pages
4 pages
publisher
Wiley-Blackwell
external identifiers
  • pmid:3838943
  • scopus:0021873193
ISSN
0014-5793
DOI
10.1016/0014-5793(85)80741-9
language
English
LU publication?
yes
id
a81ab5a2-d22f-49a0-b7db-ce92b22d70ca
date added to LUP
2019-06-13 17:23:52
date last changed
2025-10-14 10:15:21
@article{a81ab5a2-d22f-49a0-b7db-ce92b22d70ca,
  abstract     = {{<p>The kinetics of trypsin activation of pancreatic procolipase was investigated and the pH dependence of the binding of procolipase and colipase to a tributyrine-bile salt interface studied. The K<sub>m</sub> was 0.06 mM and K<sub>cat</sub> 8 s<sup>-1</sup>, and was of the same order of magnitude as for the activation of pancreatic zymogens. At basic pH values colipase had a higher affinity for the tributyrine-bile salt interface as compared to procolipase. The trypsin activation of procolipase ensures a rapid degradation of dietary lipids in the intestine.</p>}},
  author       = {{Wieloch, Tadeusz}},
  issn         = {{0014-5793}},
  keywords     = {{Colipase; Kinetics; Lipid binding; Procolipase; Trypsin activation}},
  language     = {{eng}},
  month        = {{06}},
  number       = {{1}},
  pages        = {{63--66}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{FEBS Letters}},
  title        = {{Trypsin activation of porcine procolipase. Kinetics of activation and effects on lipid binding}},
  url          = {{http://dx.doi.org/10.1016/0014-5793(85)80741-9}},
  doi          = {{10.1016/0014-5793(85)80741-9}},
  volume       = {{185}},
  year         = {{1985}},
}