Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Trypsin activation of porcine procolipase. Kinetics of activation and effects on lipid binding

Wieloch, Tadeusz LU (1985) In FEBS Letters 185(1). p.63-66
Abstract

The kinetics of trypsin activation of pancreatic procolipase was investigated and the pH dependence of the binding of procolipase and colipase to a tributyrine-bile salt interface studied. The Km was 0.06 mM and Kcat 8 s-1, and was of the same order of magnitude as for the activation of pancreatic zymogens. At basic pH values colipase had a higher affinity for the tributyrine-bile salt interface as compared to procolipase. The trypsin activation of procolipase ensures a rapid degradation of dietary lipids in the intestine.

Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Colipase, Kinetics, Lipid binding, Procolipase, Trypsin activation
in
FEBS Letters
volume
185
issue
1
pages
4 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:0021873193
  • pmid:3838943
ISSN
0014-5793
DOI
10.1016/0014-5793(85)80741-9
language
English
LU publication?
yes
id
a81ab5a2-d22f-49a0-b7db-ce92b22d70ca
date added to LUP
2019-06-13 17:23:52
date last changed
2024-01-01 10:27:01
@article{a81ab5a2-d22f-49a0-b7db-ce92b22d70ca,
  abstract     = {{<p>The kinetics of trypsin activation of pancreatic procolipase was investigated and the pH dependence of the binding of procolipase and colipase to a tributyrine-bile salt interface studied. The K<sub>m</sub> was 0.06 mM and K<sub>cat</sub> 8 s<sup>-1</sup>, and was of the same order of magnitude as for the activation of pancreatic zymogens. At basic pH values colipase had a higher affinity for the tributyrine-bile salt interface as compared to procolipase. The trypsin activation of procolipase ensures a rapid degradation of dietary lipids in the intestine.</p>}},
  author       = {{Wieloch, Tadeusz}},
  issn         = {{0014-5793}},
  keywords     = {{Colipase; Kinetics; Lipid binding; Procolipase; Trypsin activation}},
  language     = {{eng}},
  month        = {{06}},
  number       = {{1}},
  pages        = {{63--66}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{FEBS Letters}},
  title        = {{Trypsin activation of porcine procolipase. Kinetics of activation and effects on lipid binding}},
  url          = {{http://dx.doi.org/10.1016/0014-5793(85)80741-9}},
  doi          = {{10.1016/0014-5793(85)80741-9}},
  volume       = {{185}},
  year         = {{1985}},
}