Trypsin activation of porcine procolipase. Kinetics of activation and effects on lipid binding
(1985) In FEBS Letters 185(1). p.63-66- Abstract
The kinetics of trypsin activation of pancreatic procolipase was investigated and the pH dependence of the binding of procolipase and colipase to a tributyrine-bile salt interface studied. The Km was 0.06 mM and Kcat 8 s-1, and was of the same order of magnitude as for the activation of pancreatic zymogens. At basic pH values colipase had a higher affinity for the tributyrine-bile salt interface as compared to procolipase. The trypsin activation of procolipase ensures a rapid degradation of dietary lipids in the intestine.
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https://lup.lub.lu.se/record/a81ab5a2-d22f-49a0-b7db-ce92b22d70ca
- author
- Wieloch, Tadeusz LU
- organization
- publishing date
- 1985-06-03
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Colipase, Kinetics, Lipid binding, Procolipase, Trypsin activation
- in
- FEBS Letters
- volume
- 185
- issue
- 1
- pages
- 4 pages
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:3838943
- scopus:0021873193
- ISSN
- 0014-5793
- DOI
- 10.1016/0014-5793(85)80741-9
- language
- English
- LU publication?
- yes
- id
- a81ab5a2-d22f-49a0-b7db-ce92b22d70ca
- date added to LUP
- 2019-06-13 17:23:52
- date last changed
- 2024-01-01 10:27:01
@article{a81ab5a2-d22f-49a0-b7db-ce92b22d70ca, abstract = {{<p>The kinetics of trypsin activation of pancreatic procolipase was investigated and the pH dependence of the binding of procolipase and colipase to a tributyrine-bile salt interface studied. The K<sub>m</sub> was 0.06 mM and K<sub>cat</sub> 8 s<sup>-1</sup>, and was of the same order of magnitude as for the activation of pancreatic zymogens. At basic pH values colipase had a higher affinity for the tributyrine-bile salt interface as compared to procolipase. The trypsin activation of procolipase ensures a rapid degradation of dietary lipids in the intestine.</p>}}, author = {{Wieloch, Tadeusz}}, issn = {{0014-5793}}, keywords = {{Colipase; Kinetics; Lipid binding; Procolipase; Trypsin activation}}, language = {{eng}}, month = {{06}}, number = {{1}}, pages = {{63--66}}, publisher = {{Wiley-Blackwell}}, series = {{FEBS Letters}}, title = {{Trypsin activation of porcine procolipase. Kinetics of activation and effects on lipid binding}}, url = {{http://dx.doi.org/10.1016/0014-5793(85)80741-9}}, doi = {{10.1016/0014-5793(85)80741-9}}, volume = {{185}}, year = {{1985}}, }