FK506 and cyclosporin A enhance the survival of cultured and grafted rat embryonic dopamine neurons
Castilho, Roger F ; Hansson, Oskar LU
and Brundin, Patrik
LU
(2000)
In Experimental Neurology
164(1).
p.94-101
- Abstract
- We examined the effects of the immunophilin ligands and calcineurin inhibitors FK506 and cyclosporin A on the survival of rat embryonic dopamine (tyrosine hydroxylase (TH)-immunoreactive) neurons. The protective effects of FK506 and cyclosporin A were first studied in dissociated mesencephalic cell cultures subjected to serum deprivation. Significant increases in both the total number of surviving mesencephalic cells and the number of surviving TH-immunoreactive neurons were observed when FK506 or cyclosporin A was present following withdrawal of serum from the culture medium. In a second series of experiments, FK506 increased the survival of dopamine neurons when added only to a hibernation medium in which donor tissue pieces were stored... (More)
- We examined the effects of the immunophilin ligands and calcineurin inhibitors FK506 and cyclosporin A on the survival of rat embryonic dopamine (tyrosine hydroxylase (TH)-immunoreactive) neurons. The protective effects of FK506 and cyclosporin A were first studied in dissociated mesencephalic cell cultures subjected to serum deprivation. Significant increases in both the total number of surviving mesencephalic cells and the number of surviving TH-immunoreactive neurons were observed when FK506 or cyclosporin A was present following withdrawal of serum from the culture medium. In a second series of experiments, FK506 increased the survival of dopamine neurons when added only to a hibernation medium in which donor tissue pieces were stored for 7 days prior to preparation of the cultures. In a third set of experiments, we investigated the effects of FK506 and cyclosporin A on the survival of grafted rat embryonic dopamine neurons. When FK506 or cyclosporin A was present during tissue preparation and in the final mesencephalic cell suspension used for grafting, the survival of TH-immunoreactive neurons implanted in the striatum increased to around 185% of control values. In contrast, treatment of graft recipient rats, but not the graft suspension itself, with immunosuppressive doses of FK506 or cyclosporin A did not augment the survival of grafted TH-immunoreactive neurons. We conclude that administration of FK506 during storage of embryonic mesencephalic tissue and FK506 or cyclosporin A during preparation of nigral cell suspensions used for grafting can increase the survival of grafted embryonic dopamine neurons. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1117114
- author
- Castilho, Roger F
; Hansson, Oskar
LU
and Brundin, Patrik
LU
- organization
- publishing date
- 2000
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- cell death, cyclosporin A, dopamine, FK506, immunophilin ligand, intracerebral transplantation, Parkinson's disease
- in
- Experimental Neurology
- volume
- 164
- issue
- 1
- pages
- 94 - 101
- publisher
- Elsevier
- external identifiers
-
- pmid:10877919
- scopus:0033913626
- pmid:10877919
- ISSN
- 0014-4886
- DOI
- 10.1006/exnr.2000.7405
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Psychiatry/Primary Care/Public Health (013240500), Neuronal Survival (013212041)
- id
- a8da9ee7-abc5-4ae6-a843-067be1aef567 (old id 1117114)
- date added to LUP
- 2016-04-01 12:15:04
- date last changed
- 2022-03-05 21:00:21
@article{a8da9ee7-abc5-4ae6-a843-067be1aef567,
abstract = {{We examined the effects of the immunophilin ligands and calcineurin inhibitors FK506 and cyclosporin A on the survival of rat embryonic dopamine (tyrosine hydroxylase (TH)-immunoreactive) neurons. The protective effects of FK506 and cyclosporin A were first studied in dissociated mesencephalic cell cultures subjected to serum deprivation. Significant increases in both the total number of surviving mesencephalic cells and the number of surviving TH-immunoreactive neurons were observed when FK506 or cyclosporin A was present following withdrawal of serum from the culture medium. In a second series of experiments, FK506 increased the survival of dopamine neurons when added only to a hibernation medium in which donor tissue pieces were stored for 7 days prior to preparation of the cultures. In a third set of experiments, we investigated the effects of FK506 and cyclosporin A on the survival of grafted rat embryonic dopamine neurons. When FK506 or cyclosporin A was present during tissue preparation and in the final mesencephalic cell suspension used for grafting, the survival of TH-immunoreactive neurons implanted in the striatum increased to around 185% of control values. In contrast, treatment of graft recipient rats, but not the graft suspension itself, with immunosuppressive doses of FK506 or cyclosporin A did not augment the survival of grafted TH-immunoreactive neurons. We conclude that administration of FK506 during storage of embryonic mesencephalic tissue and FK506 or cyclosporin A during preparation of nigral cell suspensions used for grafting can increase the survival of grafted embryonic dopamine neurons.}},
author = {{Castilho, Roger F and Hansson, Oskar and Brundin, Patrik}},
issn = {{0014-4886}},
keywords = {{cell death; cyclosporin A; dopamine; FK506; immunophilin ligand; intracerebral transplantation; Parkinson's disease}},
language = {{eng}},
number = {{1}},
pages = {{94--101}},
publisher = {{Elsevier}},
series = {{Experimental Neurology}},
title = {{FK506 and cyclosporin A enhance the survival of cultured and grafted rat embryonic dopamine neurons}},
url = {{http://dx.doi.org/10.1006/exnr.2000.7405}},
doi = {{10.1006/exnr.2000.7405}},
volume = {{164}},
year = {{2000}},
}