On depolarization-evoked exocytosis as a function of calcium entry: possibilities and pitfalls.
(2011) In Biophysical Journal 101(4). p.793-802- Abstract
- Secretion from many endocrine cells is a result of calcium-regulated exocytosis due to Ca(2+) influx. Using the patch-clamp technique, voltage pulses can be applied to the cells to open Ca(2+) channels, resulting in a measurable Ca(2+) current, and evoke exocytosis, which can be seen as an increase in membrane capacitance. A common tool for evaluating the relation between Ca(2+) influx and exocytosis is to plot the increase in capacitance (ΔC(m)) as a function of the integral of the measured Ca(2+) current (Q). When depolarizations of different lengths are imposed, the rate of exocytosis is typically higher for shorter than for longer pulses, which has been suggested to result from depletion of a granule pool or from Ca(2+) current... (More)
- Secretion from many endocrine cells is a result of calcium-regulated exocytosis due to Ca(2+) influx. Using the patch-clamp technique, voltage pulses can be applied to the cells to open Ca(2+) channels, resulting in a measurable Ca(2+) current, and evoke exocytosis, which can be seen as an increase in membrane capacitance. A common tool for evaluating the relation between Ca(2+) influx and exocytosis is to plot the increase in capacitance (ΔC(m)) as a function of the integral of the measured Ca(2+) current (Q). When depolarizations of different lengths are imposed, the rate of exocytosis is typically higher for shorter than for longer pulses, which has been suggested to result from depletion of a granule pool or from Ca(2+) current inactivation. It is here demonstrated that ΔC(m) as a function of Q can reveal whether Ca(2+) current inactivation masquerades as pool depletion. Moreover, it is shown that a convex, cooperativity-like, relation between ΔC(m) and Q surprisingly cannot occur as a result of cooperative effects, but can result from delays in the exocytotic process or in Ca(2+) dynamics. An overview of expected ΔC(m)-versus-Q relations for a range of explicit situations is given, which should help in the interpretation of data of depolarization-evoked exocytosis in endocrine cells. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2151074
- author
- Pedersen, Morten Gram LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biophysical Journal
- volume
- 101
- issue
- 4
- pages
- 793 - 802
- publisher
- Cell Press
- external identifiers
-
- wos:000294103600008
- pmid:21843469
- scopus:80052480935
- pmid:21843469
- ISSN
- 1542-0086
- DOI
- 10.1016/j.bpj.2011.06.061
- language
- English
- LU publication?
- yes
- id
- a8e73cee-81cc-4473-b457-c174a5fe9e75 (old id 2151074)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/21843469?dopt=Abstract
- date added to LUP
- 2016-04-01 10:07:47
- date last changed
- 2022-01-25 20:02:53
@article{a8e73cee-81cc-4473-b457-c174a5fe9e75,
abstract = {{Secretion from many endocrine cells is a result of calcium-regulated exocytosis due to Ca(2+) influx. Using the patch-clamp technique, voltage pulses can be applied to the cells to open Ca(2+) channels, resulting in a measurable Ca(2+) current, and evoke exocytosis, which can be seen as an increase in membrane capacitance. A common tool for evaluating the relation between Ca(2+) influx and exocytosis is to plot the increase in capacitance (ΔC(m)) as a function of the integral of the measured Ca(2+) current (Q). When depolarizations of different lengths are imposed, the rate of exocytosis is typically higher for shorter than for longer pulses, which has been suggested to result from depletion of a granule pool or from Ca(2+) current inactivation. It is here demonstrated that ΔC(m) as a function of Q can reveal whether Ca(2+) current inactivation masquerades as pool depletion. Moreover, it is shown that a convex, cooperativity-like, relation between ΔC(m) and Q surprisingly cannot occur as a result of cooperative effects, but can result from delays in the exocytotic process or in Ca(2+) dynamics. An overview of expected ΔC(m)-versus-Q relations for a range of explicit situations is given, which should help in the interpretation of data of depolarization-evoked exocytosis in endocrine cells.}},
author = {{Pedersen, Morten Gram}},
issn = {{1542-0086}},
language = {{eng}},
number = {{4}},
pages = {{793--802}},
publisher = {{Cell Press}},
series = {{Biophysical Journal}},
title = {{On depolarization-evoked exocytosis as a function of calcium entry: possibilities and pitfalls.}},
url = {{https://lup.lub.lu.se/search/files/1586925/2342334.pdf}},
doi = {{10.1016/j.bpj.2011.06.061}},
volume = {{101}},
year = {{2011}},
}