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Design and use of model membranes to study biomolecular interactions using complementary surface-sensitive techniques

Clifton, Luke A. ; Campbell, Richard A. ; Sebastiani, Federica ; Campos-Terán, José LU ; Gonzalez-Martinez, Juan F. ; Björklund, Sebastian LU ; Sotres, Javier and Cárdenas, Marité LU (2020) In Advances in Colloid and Interface Science 277.
Abstract

Cellular membranes are complex structures and simplified analogues in the form of model membranes or biomembranes are used as platforms to understand fundamental properties of the membrane itself as well as interactions with various biomolecules such as drugs, peptides and proteins. Model membranes at the air-liquid and solid-liquid interfaces can be studied using a range of complementary surface-sensitive techniques to give a detailed picture of both the structure and physicochemical properties of the membrane and its resulting interactions. In this review, we will present the main planar model membranes used in the field to date with a focus on monolayers at the air-liquid interface, supported lipid bilayers at the solid-liquid... (More)

Cellular membranes are complex structures and simplified analogues in the form of model membranes or biomembranes are used as platforms to understand fundamental properties of the membrane itself as well as interactions with various biomolecules such as drugs, peptides and proteins. Model membranes at the air-liquid and solid-liquid interfaces can be studied using a range of complementary surface-sensitive techniques to give a detailed picture of both the structure and physicochemical properties of the membrane and its resulting interactions. In this review, we will present the main planar model membranes used in the field to date with a focus on monolayers at the air-liquid interface, supported lipid bilayers at the solid-liquid interface and advanced membrane models such as tethered and floating membranes. We will then briefly present the principles as well as the main type of information on molecular interactions at model membranes accessible using a Langmuir trough, quartz crystal microbalance with dissipation monitoring, ellipsometry, atomic force microscopy, Brewster angle microscopy, Infrared spectroscopy, and neutron and X-ray reflectometry. A consistent example for following biomolecular interactions at model membranes is used across many of the techniques in terms of the well-studied antimicrobial peptide Melittin. The overall objective is to establish an understanding of the information accessible from each technique, their respective advantages and limitations, and their complementarity.

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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Antimicrobial peptides., Biomembranes, Biomolecular interactions, Lipids, Model membrane., Surface-sensitive techniques
in
Advances in Colloid and Interface Science
volume
277
article number
102118
publisher
Elsevier
external identifiers
  • pmid:32044469
  • scopus:85078986446
ISSN
0001-8686
DOI
10.1016/j.cis.2020.102118
language
English
LU publication?
yes
id
a9324dcf-c738-498e-984d-553a0e4196f9
date added to LUP
2020-02-19 15:51:28
date last changed
2024-06-12 09:26:10
@article{a9324dcf-c738-498e-984d-553a0e4196f9,
  abstract     = {{<p>Cellular membranes are complex structures and simplified analogues in the form of model membranes or biomembranes are used as platforms to understand fundamental properties of the membrane itself as well as interactions with various biomolecules such as drugs, peptides and proteins. Model membranes at the air-liquid and solid-liquid interfaces can be studied using a range of complementary surface-sensitive techniques to give a detailed picture of both the structure and physicochemical properties of the membrane and its resulting interactions. In this review, we will present the main planar model membranes used in the field to date with a focus on monolayers at the air-liquid interface, supported lipid bilayers at the solid-liquid interface and advanced membrane models such as tethered and floating membranes. We will then briefly present the principles as well as the main type of information on molecular interactions at model membranes accessible using a Langmuir trough, quartz crystal microbalance with dissipation monitoring, ellipsometry, atomic force microscopy, Brewster angle microscopy, Infrared spectroscopy, and neutron and X-ray reflectometry. A consistent example for following biomolecular interactions at model membranes is used across many of the techniques in terms of the well-studied antimicrobial peptide Melittin. The overall objective is to establish an understanding of the information accessible from each technique, their respective advantages and limitations, and their complementarity.</p>}},
  author       = {{Clifton, Luke A. and Campbell, Richard A. and Sebastiani, Federica and Campos-Terán, José and Gonzalez-Martinez, Juan F. and Björklund, Sebastian and Sotres, Javier and Cárdenas, Marité}},
  issn         = {{0001-8686}},
  keywords     = {{Antimicrobial peptides.; Biomembranes; Biomolecular interactions; Lipids; Model membrane.; Surface-sensitive techniques}},
  language     = {{eng}},
  publisher    = {{Elsevier}},
  series       = {{Advances in Colloid and Interface Science}},
  title        = {{Design and use of model membranes to study biomolecular interactions using complementary surface-sensitive techniques}},
  url          = {{http://dx.doi.org/10.1016/j.cis.2020.102118}},
  doi          = {{10.1016/j.cis.2020.102118}},
  volume       = {{277}},
  year         = {{2020}},
}