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Lactic acid bacteria colonization and clinical outcome after probiotic supplementation in conventionally treated bacterial vaginosis and vulvovaginal candidiasis

Ehrström, Sophia; Daroczy, Katalin; Rylander, Eva; Samuelsson, Carolina LU ; Johannesson, Ulrika; Anzén, Bo and Påhlson, Carl (2010) In Microbes and Infection 12(10). p.9-691
Abstract

This randomized double-blind placebo controlled study assessed the vaginal colonization of lactic acid bacteria and clinical outcome. Vaginal capsules containing L gasseri LN40, Lactobacillus fermentum LN99, L. casei subsp. rhamnosus LN113 and P. acidilactici LN23, or placebos were administered for five days to 95 women after conventional treatment of bacterial vaginosis and/or vulvovaginal candidiasis. Vulvovaginal examinations and vaginal samplings were performed before and after administration, after the first and second menstruation, and after six months. Presence of LN strains was assessed using RAPD analysis. LN strains were present 2-3 days after administration in 89% of the women receiving LN strains (placebo: 0%, p <... (More)

This randomized double-blind placebo controlled study assessed the vaginal colonization of lactic acid bacteria and clinical outcome. Vaginal capsules containing L gasseri LN40, Lactobacillus fermentum LN99, L. casei subsp. rhamnosus LN113 and P. acidilactici LN23, or placebos were administered for five days to 95 women after conventional treatment of bacterial vaginosis and/or vulvovaginal candidiasis. Vulvovaginal examinations and vaginal samplings were performed before and after administration, after the first and second menstruation, and after six months. Presence of LN strains was assessed using RAPD analysis. LN strains were present 2-3 days after administration in 89% of the women receiving LN strains (placebo: 0%, p < 0.0001). After one menstruation 53% were colonized by at least one LN strain. Nine percent were still colonized six months after administration. Ninety-three percent of the women receiving LN strains were cured 2-3 days after administration (placebo: 83%), and 78% after one menstruation (placebo: 71%) (ns). The intervention group experienced less malodorous discharge 2-3 days after administration (p = 0.03) and after the second menstruation (p = 0.04), compared with placebo. In summary, five days of vaginal administration of LN strains after conventional treatment of bacterial vaginosis and/or vulvovaginal candidiasis lead to vaginal colonization, somewhat fewer recurrences and less malodorous discharge.

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publication status
published
subject
keywords
Adolescent, Adult, Anti-Infective Agents/administration & dosage, Candidiasis, Vulvovaginal/drug therapy, DNA Fingerprinting, DNA, Bacterial/genetics, Double-Blind Method, Female, Humans, Lactobacillus/classification, Middle Aged, Pediococcus/classification, Probiotics/administration & dosage, Random Amplified Polymorphic DNA Technique, Secondary Prevention, Treatment Outcome, Vagina/microbiology, Vaginosis, Bacterial/drug therapy, Vulva/microbiology, Young Adult
in
Microbes and Infection
volume
12
issue
10
pages
9 pages
publisher
Elsevier
external identifiers
  • scopus:77956264287
ISSN
1769-714X
DOI
10.1016/j.micinf.2010.04.010
language
English
LU publication?
no
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a93bbd85-c433-4c5b-9044-042dc8c984b2
date added to LUP
2019-06-05 16:07:15
date last changed
2019-10-23 06:17:40
@article{a93bbd85-c433-4c5b-9044-042dc8c984b2,
  abstract     = {<p>This randomized double-blind placebo controlled study assessed the vaginal colonization of lactic acid bacteria and clinical outcome. Vaginal capsules containing L gasseri LN40, Lactobacillus fermentum LN99, L. casei subsp. rhamnosus LN113 and P. acidilactici LN23, or placebos were administered for five days to 95 women after conventional treatment of bacterial vaginosis and/or vulvovaginal candidiasis. Vulvovaginal examinations and vaginal samplings were performed before and after administration, after the first and second menstruation, and after six months. Presence of LN strains was assessed using RAPD analysis. LN strains were present 2-3 days after administration in 89% of the women receiving LN strains (placebo: 0%, p &lt; 0.0001). After one menstruation 53% were colonized by at least one LN strain. Nine percent were still colonized six months after administration. Ninety-three percent of the women receiving LN strains were cured 2-3 days after administration (placebo: 83%), and 78% after one menstruation (placebo: 71%) (ns). The intervention group experienced less malodorous discharge 2-3 days after administration (p = 0.03) and after the second menstruation (p = 0.04), compared with placebo. In summary, five days of vaginal administration of LN strains after conventional treatment of bacterial vaginosis and/or vulvovaginal candidiasis lead to vaginal colonization, somewhat fewer recurrences and less malodorous discharge.</p>},
  author       = {Ehrström, Sophia and Daroczy, Katalin and Rylander, Eva and Samuelsson, Carolina and Johannesson, Ulrika and Anzén, Bo and Påhlson, Carl},
  issn         = {1769-714X},
  keyword      = {Adolescent,Adult,Anti-Infective Agents/administration & dosage,Candidiasis, Vulvovaginal/drug therapy,DNA Fingerprinting,DNA, Bacterial/genetics,Double-Blind Method,Female,Humans,Lactobacillus/classification,Middle Aged,Pediococcus/classification,Probiotics/administration & dosage,Random Amplified Polymorphic DNA Technique,Secondary Prevention,Treatment Outcome,Vagina/microbiology,Vaginosis, Bacterial/drug therapy,Vulva/microbiology,Young Adult},
  language     = {eng},
  number       = {10},
  pages        = {9--691},
  publisher    = {Elsevier},
  series       = {Microbes and Infection},
  title        = {Lactic acid bacteria colonization and clinical outcome after probiotic supplementation in conventionally treated bacterial vaginosis and vulvovaginal candidiasis},
  url          = {http://dx.doi.org/10.1016/j.micinf.2010.04.010},
  volume       = {12},
  year         = {2010},
}