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Overexpression of ST6GalNAcV, a ganglioside-specific alpha2,6-sialyltransferase, inhibits glioma growth in vivo

Kroes, Roger A; He, Huan; Emmett, Mark R; Nilsson, Carol L LU ; Leach, Franklin E; Amster, I Jonathan; Marshall, Alan G and Moskal, Joseph R (2010) In Proceedings of the National Academy of Sciences of the United States of America 107(28). p.51-12646
Abstract

Aberrant cell-surface glycosylation patterns are present on virtually all tumors and have been linked to tumor progression, metastasis, and invasivity. We have shown that expressing a normally quiescent, glycoprotein-specific alpha2,6-sialyltransferase (ST6Gal1) gene in gliomas inhibited invasivity in vitro and tumor formation in vivo. To identify other glycogene targets with therapeutic potential, we created a focused 45-mer oligonucleotide microarray platform representing all of the cloned human glycotranscriptome and examined the glycogene expression profiles of 10 normal human brain specimens, 10 malignant gliomas, and 7 human glioma cell lines. Among the many significant changes in glycogene expression observed, of particular... (More)

Aberrant cell-surface glycosylation patterns are present on virtually all tumors and have been linked to tumor progression, metastasis, and invasivity. We have shown that expressing a normally quiescent, glycoprotein-specific alpha2,6-sialyltransferase (ST6Gal1) gene in gliomas inhibited invasivity in vitro and tumor formation in vivo. To identify other glycogene targets with therapeutic potential, we created a focused 45-mer oligonucleotide microarray platform representing all of the cloned human glycotranscriptome and examined the glycogene expression profiles of 10 normal human brain specimens, 10 malignant gliomas, and 7 human glioma cell lines. Among the many significant changes in glycogene expression observed, of particular interest was the observation that an additional alpha2,6-sialyltransferase, ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha2,6-sialyltransferase 5 (ST6GalNAcV), was expressed at very low levels in all glioma and glioma cell lines examined compared with normal brain. ST6GalNAcV catalyzes the formation of the terminal alpha2,6-sialic acid linkages on gangliosides. Stable transfection of ST6GalNAcV into U373MG glioma cells produced (i) no change in alpha2,6-linked sialic acid-containing glycoproteins, (ii) increased expression of GM2alpha and GM3 gangliosides and decreased expression of GM1b, Gb3, and Gb4, (iii) marked inhibition of in vitro invasivity, (iv) modified cellular adhesion to fibronectin and laminin, (v) increased adhesion-mediated protein tyrosine phosphorylation of HSPA8, and (vi) inhibition of tumor growth in vivo. These results strongly suggest that modulation of the synthesis of specific glioma cell-surface glycosphingolipids alters invasivity in a manner that may have significant therapeutic potential.

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publication status
published
keywords
Animals, Biochemical Phenomena, Brain, Cell Adhesion, Cell Line, Cell Membrane, Fibronectins, Gangliosides, Genes, Glioma, Glycoproteins, Glycosylation, Humans, Mice, Mice, SCID, N-Acetylneuraminic Acid, Oligonucleotide Array Sequence Analysis, Phosphorylation, Sialyltransferases, Transfection, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.
in
Proceedings of the National Academy of Sciences of the United States of America
volume
107
issue
28
pages
6 pages
publisher
National Acad Sciences
external identifiers
  • scopus:77955462802
ISSN
1091-6490
DOI
10.1073/pnas.0909862107
language
English
LU publication?
no
id
a9d82ec1-f6e8-48c0-9e86-ffa33a7ea3ea
date added to LUP
2017-05-16 10:29:45
date last changed
2018-07-01 04:45:28
@article{a9d82ec1-f6e8-48c0-9e86-ffa33a7ea3ea,
  abstract     = {<p>Aberrant cell-surface glycosylation patterns are present on virtually all tumors and have been linked to tumor progression, metastasis, and invasivity. We have shown that expressing a normally quiescent, glycoprotein-specific alpha2,6-sialyltransferase (ST6Gal1) gene in gliomas inhibited invasivity in vitro and tumor formation in vivo. To identify other glycogene targets with therapeutic potential, we created a focused 45-mer oligonucleotide microarray platform representing all of the cloned human glycotranscriptome and examined the glycogene expression profiles of 10 normal human brain specimens, 10 malignant gliomas, and 7 human glioma cell lines. Among the many significant changes in glycogene expression observed, of particular interest was the observation that an additional alpha2,6-sialyltransferase, ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha2,6-sialyltransferase 5 (ST6GalNAcV), was expressed at very low levels in all glioma and glioma cell lines examined compared with normal brain. ST6GalNAcV catalyzes the formation of the terminal alpha2,6-sialic acid linkages on gangliosides. Stable transfection of ST6GalNAcV into U373MG glioma cells produced (i) no change in alpha2,6-linked sialic acid-containing glycoproteins, (ii) increased expression of GM2alpha and GM3 gangliosides and decreased expression of GM1b, Gb3, and Gb4, (iii) marked inhibition of in vitro invasivity, (iv) modified cellular adhesion to fibronectin and laminin, (v) increased adhesion-mediated protein tyrosine phosphorylation of HSPA8, and (vi) inhibition of tumor growth in vivo. These results strongly suggest that modulation of the synthesis of specific glioma cell-surface glycosphingolipids alters invasivity in a manner that may have significant therapeutic potential.</p>},
  author       = {Kroes, Roger A and He, Huan and Emmett, Mark R and Nilsson, Carol L and Leach, Franklin E and Amster, I Jonathan and Marshall, Alan G and Moskal, Joseph R},
  issn         = {1091-6490},
  keyword      = {Animals,Biochemical Phenomena,Brain,Cell Adhesion,Cell Line,Cell Membrane,Fibronectins,Gangliosides,Genes,Glioma,Glycoproteins,Glycosylation,Humans,Mice,Mice, SCID,N-Acetylneuraminic Acid,Oligonucleotide Array Sequence Analysis,Phosphorylation,Sialyltransferases,Transfection,Journal Article,Research Support, Non-U.S. Gov't,Research Support, U.S. Gov't, Non-P.H.S.},
  language     = {eng},
  month        = {07},
  number       = {28},
  pages        = {51--12646},
  publisher    = {National Acad Sciences},
  series       = {Proceedings of the National Academy of Sciences of the United States of America},
  title        = {Overexpression of ST6GalNAcV, a ganglioside-specific alpha2,6-sialyltransferase, inhibits glioma growth in vivo},
  url          = {http://dx.doi.org/10.1073/pnas.0909862107},
  volume       = {107},
  year         = {2010},
}