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Increased TLR7 expression in the adenoids among children with otitis media with effusion

Granath, Anna ; Uddman, Rolf LU and Cardell, Lars-Olaf (2010) In Acta Oto-Laryngologica 130(1). p.57-61
Abstract
Conclusion: Toll-like receptor 7 (TLR7) is present in the adenoids in young children and might play a role in the immunological response behind the development of otitis media with effusion (OME). Objectives: To investigate the expression of the TLRs TLR4 and TLR7 in adenoids from children with OME and to compare the results with data obtained from healthy controls. Subjects and methods: This was a controlled, prospective study. Eleven young children with long-standing OME and 10 controls with healthy middle ears were recruited consecutively when scheduled for adenoidectomy. mRNA was quantified using real-time polymerase chain reaction (PCR) and the localization of the corresponding proteins was assessed by immunohistochemistry. Results:... (More)
Conclusion: Toll-like receptor 7 (TLR7) is present in the adenoids in young children and might play a role in the immunological response behind the development of otitis media with effusion (OME). Objectives: To investigate the expression of the TLRs TLR4 and TLR7 in adenoids from children with OME and to compare the results with data obtained from healthy controls. Subjects and methods: This was a controlled, prospective study. Eleven young children with long-standing OME and 10 controls with healthy middle ears were recruited consecutively when scheduled for adenoidectomy. mRNA was quantified using real-time polymerase chain reaction (PCR) and the localization of the corresponding proteins was assessed by immunohistochemistry. Results: mRNA for TLR4 and TLR7 could be obtained from all samples tested along with their corresponding proteins. The mRNA levels for TLR7 were increased among the children with a history of OME. No such increase was found for TLR4. (Less)
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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Streptococcus pneumoniae, Haemophilus influenzae, RT-PCR, TLR4, immunohistochemistry
in
Acta Oto-Laryngologica
volume
130
issue
1
pages
57 - 61
publisher
Taylor & Francis
external identifiers
  • wos:000274416300010
  • scopus:75449108827
  • pmid:19452306
ISSN
1651-2251
DOI
10.3109/00016480902963061
language
English
LU publication?
yes
id
a9e4e740-6881-4e31-981d-9008fb8cc416 (old id 1569016)
date added to LUP
2016-04-01 15:05:47
date last changed
2022-02-05 00:22:28
@article{a9e4e740-6881-4e31-981d-9008fb8cc416,
  abstract     = {{Conclusion: Toll-like receptor 7 (TLR7) is present in the adenoids in young children and might play a role in the immunological response behind the development of otitis media with effusion (OME). Objectives: To investigate the expression of the TLRs TLR4 and TLR7 in adenoids from children with OME and to compare the results with data obtained from healthy controls. Subjects and methods: This was a controlled, prospective study. Eleven young children with long-standing OME and 10 controls with healthy middle ears were recruited consecutively when scheduled for adenoidectomy. mRNA was quantified using real-time polymerase chain reaction (PCR) and the localization of the corresponding proteins was assessed by immunohistochemistry. Results: mRNA for TLR4 and TLR7 could be obtained from all samples tested along with their corresponding proteins. The mRNA levels for TLR7 were increased among the children with a history of OME. No such increase was found for TLR4.}},
  author       = {{Granath, Anna and Uddman, Rolf and Cardell, Lars-Olaf}},
  issn         = {{1651-2251}},
  keywords     = {{Streptococcus pneumoniae; Haemophilus influenzae; RT-PCR; TLR4; immunohistochemistry}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{57--61}},
  publisher    = {{Taylor & Francis}},
  series       = {{Acta Oto-Laryngologica}},
  title        = {{Increased TLR7 expression in the adenoids among children with otitis media with effusion}},
  url          = {{http://dx.doi.org/10.3109/00016480902963061}},
  doi          = {{10.3109/00016480902963061}},
  volume       = {{130}},
  year         = {{2010}},
}