Increased TLR7 expression in the adenoids among children with otitis media with effusion
(2010) In Acta Oto-Laryngologica 130(1). p.57-61- Abstract
- Conclusion: Toll-like receptor 7 (TLR7) is present in the adenoids in young children and might play a role in the immunological response behind the development of otitis media with effusion (OME). Objectives: To investigate the expression of the TLRs TLR4 and TLR7 in adenoids from children with OME and to compare the results with data obtained from healthy controls. Subjects and methods: This was a controlled, prospective study. Eleven young children with long-standing OME and 10 controls with healthy middle ears were recruited consecutively when scheduled for adenoidectomy. mRNA was quantified using real-time polymerase chain reaction (PCR) and the localization of the corresponding proteins was assessed by immunohistochemistry. Results:... (More)
- Conclusion: Toll-like receptor 7 (TLR7) is present in the adenoids in young children and might play a role in the immunological response behind the development of otitis media with effusion (OME). Objectives: To investigate the expression of the TLRs TLR4 and TLR7 in adenoids from children with OME and to compare the results with data obtained from healthy controls. Subjects and methods: This was a controlled, prospective study. Eleven young children with long-standing OME and 10 controls with healthy middle ears were recruited consecutively when scheduled for adenoidectomy. mRNA was quantified using real-time polymerase chain reaction (PCR) and the localization of the corresponding proteins was assessed by immunohistochemistry. Results: mRNA for TLR4 and TLR7 could be obtained from all samples tested along with their corresponding proteins. The mRNA levels for TLR7 were increased among the children with a history of OME. No such increase was found for TLR4. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1569016
- author
- Granath, Anna ; Uddman, Rolf LU and Cardell, Lars-Olaf
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Streptococcus pneumoniae, Haemophilus influenzae, RT-PCR, TLR4, immunohistochemistry
- in
- Acta Oto-Laryngologica
- volume
- 130
- issue
- 1
- pages
- 57 - 61
- publisher
- Taylor & Francis
- external identifiers
-
- wos:000274416300010
- scopus:75449108827
- pmid:19452306
- ISSN
- 1651-2251
- DOI
- 10.3109/00016480902963061
- language
- English
- LU publication?
- yes
- id
- a9e4e740-6881-4e31-981d-9008fb8cc416 (old id 1569016)
- date added to LUP
- 2016-04-01 15:05:47
- date last changed
- 2022-02-05 00:22:28
@article{a9e4e740-6881-4e31-981d-9008fb8cc416, abstract = {{Conclusion: Toll-like receptor 7 (TLR7) is present in the adenoids in young children and might play a role in the immunological response behind the development of otitis media with effusion (OME). Objectives: To investigate the expression of the TLRs TLR4 and TLR7 in adenoids from children with OME and to compare the results with data obtained from healthy controls. Subjects and methods: This was a controlled, prospective study. Eleven young children with long-standing OME and 10 controls with healthy middle ears were recruited consecutively when scheduled for adenoidectomy. mRNA was quantified using real-time polymerase chain reaction (PCR) and the localization of the corresponding proteins was assessed by immunohistochemistry. Results: mRNA for TLR4 and TLR7 could be obtained from all samples tested along with their corresponding proteins. The mRNA levels for TLR7 were increased among the children with a history of OME. No such increase was found for TLR4.}}, author = {{Granath, Anna and Uddman, Rolf and Cardell, Lars-Olaf}}, issn = {{1651-2251}}, keywords = {{Streptococcus pneumoniae; Haemophilus influenzae; RT-PCR; TLR4; immunohistochemistry}}, language = {{eng}}, number = {{1}}, pages = {{57--61}}, publisher = {{Taylor & Francis}}, series = {{Acta Oto-Laryngologica}}, title = {{Increased TLR7 expression in the adenoids among children with otitis media with effusion}}, url = {{http://dx.doi.org/10.3109/00016480902963061}}, doi = {{10.3109/00016480902963061}}, volume = {{130}}, year = {{2010}}, }