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Organisation of genes for tetrapyrrole biosynthesis in Gram-positive bacteria

Johansson, Per LU and Hederstedt, Lars LU (1999) In Microbiology 154. p.529-538
Abstract
Clusters of genes encoding enzymes for tetrapyrrole biosynthesis were cloned from Bacillus sphaericus, Bacillus stearothermophilus, Brevibacillus brevis and Paenibacillus macerans. The sequences of all hemX genes found, and of a 6.3 kbp hem gene cluster from P. macerans, were determined. The structure of the hem gene clusters was compared to that of other Gram-positive bacteria. The Bacillus and Brevibacillus species have a conserved organization of the genes hemAXCDBL, required for biosynthesis of uroporphyrinogen III (UroIII) from glutamyl-tRNA. In P. macerans, the hem genes for UroIII synthesis are also closely linked but their organization is different: there is no hemX gene and the gene cluster also contains genes, cysC(B) and... (More)
Clusters of genes encoding enzymes for tetrapyrrole biosynthesis were cloned from Bacillus sphaericus, Bacillus stearothermophilus, Brevibacillus brevis and Paenibacillus macerans. The sequences of all hemX genes found, and of a 6.3 kbp hem gene cluster from P. macerans, were determined. The structure of the hem gene clusters was compared to that of other Gram-positive bacteria. The Bacillus and Brevibacillus species have a conserved organization of the genes hemAXCDBL, required for biosynthesis of uroporphyrinogen III (UroIII) from glutamyl-tRNA. In P. macerans, the hem genes for UroIII synthesis are also closely linked but their organization is different: there is no hemX gene and the gene cluster also contains genes, cysC(B) and cysG(A)-hemD, encoding the enzymes required for synthesis of sirohaem from UroIII. Bacillus subtilis contains genes for three proteins, NasF, YlnD and YlnF, with sequence similarity to Escherichia coli CysG, which is a multi-functional protein catalysing sirohaem synthesis from UroIII. It is shown that YlnF is required for sirohaem synthesis and probably catalyses the precorrin-2 to sirohaem conversion. YlnD probably catalyses precorrin-2 synthesis from UroIII and NasF seems to be specific for nitrite reduction. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
hem genes, haem synthesis, sirohaem, tetrapyrrole synthesis, Bacillus
in
Microbiology
volume
154
pages
529 - 538
publisher
MAIK Nauka/Interperiodica
ISSN
1465-2080
DOI
10.1099/13500872-145-3-529
language
English
LU publication?
yes
id
a9e9c7fe-2502-4de7-97ed-009a00aac8f2
date added to LUP
2017-07-18 09:45:29
date last changed
2017-08-11 11:43:22
@article{a9e9c7fe-2502-4de7-97ed-009a00aac8f2,
  abstract     = {Clusters of genes encoding enzymes for tetrapyrrole biosynthesis were cloned from Bacillus sphaericus, Bacillus stearothermophilus, Brevibacillus brevis and Paenibacillus macerans. The sequences of all hemX genes found, and of a 6.3 kbp hem gene cluster from P. macerans, were determined. The structure of the hem gene clusters was compared to that of other Gram-positive bacteria. The Bacillus and Brevibacillus species have a conserved organization of the genes hemAXCDBL, required for biosynthesis of uroporphyrinogen III (UroIII) from glutamyl-tRNA. In P. macerans, the hem genes for UroIII synthesis are also closely linked but their organization is different: there is no hemX gene and the gene cluster also contains genes, cysC(B) and cysG(A)-hemD, encoding the enzymes required for synthesis of sirohaem from UroIII. Bacillus subtilis contains genes for three proteins, NasF, YlnD and YlnF, with sequence similarity to Escherichia coli CysG, which is a multi-functional protein catalysing sirohaem synthesis from UroIII. It is shown that YlnF is required for sirohaem synthesis and probably catalyses the precorrin-2 to sirohaem conversion. YlnD probably catalyses precorrin-2 synthesis from UroIII and NasF seems to be specific for nitrite reduction.},
  author       = {Johansson, Per and Hederstedt, Lars},
  issn         = {1465-2080},
  keyword      = {hem genes,haem synthesis, sirohaem,tetrapyrrole synthesis,Bacillus},
  language     = {eng},
  pages        = {529--538},
  publisher    = {MAIK Nauka/Interperiodica},
  series       = {Microbiology},
  title        = {Organisation of genes for tetrapyrrole biosynthesis in Gram-positive bacteria},
  url          = {http://dx.doi.org/10.1099/13500872-145-3-529},
  volume       = {154},
  year         = {1999},
}