Generation of single-copy Transposon insertions in Clostridium perfringens by electroporation of phage mu dna transposition complexes
(2009) In Applied and Environmental Microbiology 75(9). p.2638-2642- Abstract
Transposon mutagenesis is a tool that is widely used for the identification of genes involved in the virulence of bacteria. Until now, transposon mutagenesis in Clostridium perfringens has been restricted to the use of Tn916-based methods with laboratory reference strains. This system yields primarily multiple transposon insertions in a single genome, thus compromising its use for the identification of virulence genes. The current study describes a new protocol for transposon mutagenesis in C. perfringens, which is based on the bacteriophage Mu transposition system. The protocol was successfully used to generate a single-insertion mutant library both for a laboratory strain and for a field isolate. Thus, it can be used as a tool in... (More)
Transposon mutagenesis is a tool that is widely used for the identification of genes involved in the virulence of bacteria. Until now, transposon mutagenesis in Clostridium perfringens has been restricted to the use of Tn916-based methods with laboratory reference strains. This system yields primarily multiple transposon insertions in a single genome, thus compromising its use for the identification of virulence genes. The current study describes a new protocol for transposon mutagenesis in C. perfringens, which is based on the bacteriophage Mu transposition system. The protocol was successfully used to generate a single-insertion mutant library both for a laboratory strain and for a field isolate. Thus, it can be used as a tool in large-scale screening to identify virulence genes of C. perfringens.
(Less)
- author
- Lanckriet, A. ; Timbermont, L. ; Happonen, L. J. LU ; Pajunen, M. I. ; Pasmans, F. ; Haesebrouck, F. ; Ducatelle, R. ; Savilahti, H. and Van Immerseel, F.
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- in
- Applied and Environmental Microbiology
- volume
- 75
- issue
- 9
- pages
- 2638 - 2642
- publisher
- American Society for Microbiology
- external identifiers
-
- pmid:19270116
- scopus:65549098002
- ISSN
- 0099-2240
- DOI
- 10.1128/AEM.02214-08
- language
- English
- LU publication?
- no
- id
- aa183fee-8b05-4c83-a376-55d04db8fad9
- date added to LUP
- 2021-11-22 05:35:57
- date last changed
- 2024-01-05 20:51:16
@article{aa183fee-8b05-4c83-a376-55d04db8fad9,
abstract = {{<p>Transposon mutagenesis is a tool that is widely used for the identification of genes involved in the virulence of bacteria. Until now, transposon mutagenesis in Clostridium perfringens has been restricted to the use of Tn916-based methods with laboratory reference strains. This system yields primarily multiple transposon insertions in a single genome, thus compromising its use for the identification of virulence genes. The current study describes a new protocol for transposon mutagenesis in C. perfringens, which is based on the bacteriophage Mu transposition system. The protocol was successfully used to generate a single-insertion mutant library both for a laboratory strain and for a field isolate. Thus, it can be used as a tool in large-scale screening to identify virulence genes of C. perfringens.</p>}},
author = {{Lanckriet, A. and Timbermont, L. and Happonen, L. J. and Pajunen, M. I. and Pasmans, F. and Haesebrouck, F. and Ducatelle, R. and Savilahti, H. and Van Immerseel, F.}},
issn = {{0099-2240}},
language = {{eng}},
number = {{9}},
pages = {{2638--2642}},
publisher = {{American Society for Microbiology}},
series = {{Applied and Environmental Microbiology}},
title = {{Generation of single-copy Transposon insertions in Clostridium perfringens by electroporation of phage mu dna transposition complexes}},
url = {{http://dx.doi.org/10.1128/AEM.02214-08}},
doi = {{10.1128/AEM.02214-08}},
volume = {{75}},
year = {{2009}},
}