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Extraction and preconcentration of salbutamol and terbutaline from aqueous samples using hollow fiber supported liquid membrane containing anionic carrier

Yamini, Yadollah LU ; Reimann, Curt LU ; Vatanara, Alireza and Jönsson, Jan Åke LU (2006) In Journal of Chromatography A 1124(1-2). p.57-67
Abstract
This paper presents a new three-phase liquid-phase microextraction (LPME) strategy for extraction and preconcentration of salbutamol (SB) and terbutaline (TB) from aqueous samples, including urine. The drugs were extracted from I I ml of aqueous sample (source phase; SP) into an organic phase with microliter volume located inside the pores of a polypropylene hollow fiber, and then back-extracted into 24 mu l of a second aqueous solution as the receiving phase (RP), located in the lumen of the hollow fiber. In preliminary experiments, we tried to transport the drugs using a pH gradient between the two sides of the hollow fiber. Due to the existence of both amine and phenolic groups on the drugs, very little transport occurred and enrichment... (More)
This paper presents a new three-phase liquid-phase microextraction (LPME) strategy for extraction and preconcentration of salbutamol (SB) and terbutaline (TB) from aqueous samples, including urine. The drugs were extracted from I I ml of aqueous sample (source phase; SP) into an organic phase with microliter volume located inside the pores of a polypropylene hollow fiber, and then back-extracted into 24 mu l of a second aqueous solution as the receiving phase (RP), located in the lumen of the hollow fiber. In preliminary experiments, we tried to transport the drugs using a pH gradient between the two sides of the hollow fiber. Due to the existence of both amine and phenolic groups on the drugs, very little transport occurred and enrichment factors (EF) less than one were obtained. Further experiments were done in the presence of bis(2-ethylhexyl) monohydrogenphosphoric acid (D2EHPA) or methyltrioctylammonium chloride (Aliquat 336) in the organic phase, to extract drugs from acidic and basic matrices, respectively. Results showed that transport of drugs from alkaline solution into I M of sodium bromide occurred when the membrane was impregnated with dihexyl ether containing 20% Aliquat 336. To optimize the EF, the effects of different parameters such as the nature of organic solvent used to impregnate the membrane, compositions and volumes of SP and RP, type and concentration of carrier, extraction time and stirring rate were investigated. Optimal results were obtained in the presence of 0.005 M of NaOH (pH 11.70) in the SP, I M of NaBr in the RP, 20% of Aliquat 336 in dihexyl ether as membrane impregnation solvent, stirring rate of 500 rpm and extraction time of 60 min. Under these conditions. enrichment factors of 52.9 and 213.1, dynamic linear ranges of 20-5000 and 10-5000, and limits of detection of 2.5 and 0.5 ng/ml were obtained for salbutamol and terbutaline, respectively. Also determination of drugs in environmental water and urine samples in the range of nanograms per millilitre with RSDs < 10% was possible using HPLC-photodiode array detection or HPLC-MS. (c) 2006 Elsevier B.V. All fights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
salbutamol, three-phase LPME, urine, hollow fiber membranes, HPLC-MS, water analysis, terbutaline
in
Journal of Chromatography A
volume
1124
issue
1-2
pages
57 - 67
publisher
Elsevier
external identifiers
  • pmid:16716341
  • wos:000239706200009
  • scopus:33746523778
ISSN
0021-9673
DOI
10.1016/j.chroma.2006.05.001
language
English
LU publication?
yes
id
aa1afdcf-83bf-452d-8c7b-b763ebd435f9 (old id 397387)
date added to LUP
2007-12-05 12:37:16
date last changed
2019-09-26 02:44:48
@article{aa1afdcf-83bf-452d-8c7b-b763ebd435f9,
  abstract     = {This paper presents a new three-phase liquid-phase microextraction (LPME) strategy for extraction and preconcentration of salbutamol (SB) and terbutaline (TB) from aqueous samples, including urine. The drugs were extracted from I I ml of aqueous sample (source phase; SP) into an organic phase with microliter volume located inside the pores of a polypropylene hollow fiber, and then back-extracted into 24 mu l of a second aqueous solution as the receiving phase (RP), located in the lumen of the hollow fiber. In preliminary experiments, we tried to transport the drugs using a pH gradient between the two sides of the hollow fiber. Due to the existence of both amine and phenolic groups on the drugs, very little transport occurred and enrichment factors (EF) less than one were obtained. Further experiments were done in the presence of bis(2-ethylhexyl) monohydrogenphosphoric acid (D2EHPA) or methyltrioctylammonium chloride (Aliquat 336) in the organic phase, to extract drugs from acidic and basic matrices, respectively. Results showed that transport of drugs from alkaline solution into I M of sodium bromide occurred when the membrane was impregnated with dihexyl ether containing 20% Aliquat 336. To optimize the EF, the effects of different parameters such as the nature of organic solvent used to impregnate the membrane, compositions and volumes of SP and RP, type and concentration of carrier, extraction time and stirring rate were investigated. Optimal results were obtained in the presence of 0.005 M of NaOH (pH 11.70) in the SP, I M of NaBr in the RP, 20% of Aliquat 336 in dihexyl ether as membrane impregnation solvent, stirring rate of 500 rpm and extraction time of 60 min. Under these conditions. enrichment factors of 52.9 and 213.1, dynamic linear ranges of 20-5000 and 10-5000, and limits of detection of 2.5 and 0.5 ng/ml were obtained for salbutamol and terbutaline, respectively. Also determination of drugs in environmental water and urine samples in the range of nanograms per millilitre with RSDs &lt; 10% was possible using HPLC-photodiode array detection or HPLC-MS. (c) 2006 Elsevier B.V. All fights reserved.},
  author       = {Yamini, Yadollah and Reimann, Curt and Vatanara, Alireza and Jönsson, Jan Åke},
  issn         = {0021-9673},
  keyword      = {salbutamol,three-phase LPME,urine,hollow fiber membranes,HPLC-MS,water analysis,terbutaline},
  language     = {eng},
  number       = {1-2},
  pages        = {57--67},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography A},
  title        = {Extraction and preconcentration of salbutamol and terbutaline from aqueous samples using hollow fiber supported liquid membrane containing anionic carrier},
  url          = {http://dx.doi.org/10.1016/j.chroma.2006.05.001},
  volume       = {1124},
  year         = {2006},
}