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Enhanced selective oxidation of trimethylolpropane to 2,2-bis(hydroxymethyl)butyric acid using Corynebacterium sp. ATCC 21245

Sayed, Mahmoud LU ; Dishisha, Tarek LU ; Sayed, Waiel F; Salem, Wesam M A; Temerk, Hanan M. and Pyo, Sang Hyun LU (2017) In Process Biochemistry
Abstract

2,2-Bis(hydroxymethyl)butyric acid (BHMB) is an important multifunctional chemical for the emerging bio-based polymer industry. It can be produced from trimethylolpropane (TMP) by selective oxidation using growing cells of Corynebacterium sp. ATCC 21245. However, this process is limited by the low volumetric productivity and low concentration of the final product. In the present study, we performed sequential batch operation with cell recycling in media containing glycerol, acetic acid, and increasing concentrations of yeast extract. This approach enhanced the conversion of 10 and 15g/L TMP to 11.0 and 16.3g/L BHMB at rates of 0.50 and 0.20g/L.h, respectively. Applying a cell bleeding strategy resulted in an overall 10-fold... (More)

2,2-Bis(hydroxymethyl)butyric acid (BHMB) is an important multifunctional chemical for the emerging bio-based polymer industry. It can be produced from trimethylolpropane (TMP) by selective oxidation using growing cells of Corynebacterium sp. ATCC 21245. However, this process is limited by the low volumetric productivity and low concentration of the final product. In the present study, we performed sequential batch operation with cell recycling in media containing glycerol, acetic acid, and increasing concentrations of yeast extract. This approach enhanced the conversion of 10 and 15g/L TMP to 11.0 and 16.3g/L BHMB at rates of 0.50 and 0.20g/L.h, respectively. Applying a cell bleeding strategy resulted in an overall 10-fold improvement in productivity. The consequently prolonged biocatalyst viability resulted in a quantitative conversion of 20g/L TMP to 22.3g/L BHMB and a yield of 1.10gBHMB/gTMP (100% molar yield). This work facilitates further studies of the selective oxidation on other industrially important polyols.

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Contribution to journal
publication status
epub
subject
keywords
2,2-Bis(hydroxymethyl)butyric acid, Cell bleeding, Cell recycling, Corynebacterium sp., Selective oxidation, Sequential batch fermentation, Trimethylolpropane
in
Process Biochemistry
publisher
Elsevier
external identifiers
  • scopus:85028041620
ISSN
1359-5113
DOI
10.1016/j.procbio.2017.08.007
language
English
LU publication?
yes
id
aa22f8fc-be31-43eb-afc9-db2f67a6aa03
date added to LUP
2017-09-15 12:04:04
date last changed
2017-09-15 12:04:04
@article{aa22f8fc-be31-43eb-afc9-db2f67a6aa03,
  abstract     = {<p>2,2-Bis(hydroxymethyl)butyric acid (BHMB) is an important multifunctional chemical for the emerging bio-based polymer industry. It can be produced from trimethylolpropane (TMP) by selective oxidation using growing cells of Corynebacterium sp. ATCC 21245. However, this process is limited by the low volumetric productivity and low concentration of the final product. In the present study, we performed sequential batch operation with cell recycling in media containing glycerol, acetic acid, and increasing concentrations of yeast extract. This approach enhanced the conversion of 10 and 15g/L TMP to 11.0 and 16.3g/L BHMB at rates of 0.50 and 0.20g/L<sup>.</sup>h, respectively. Applying a cell bleeding strategy resulted in an overall 10-fold improvement in productivity. The consequently prolonged biocatalyst viability resulted in a quantitative conversion of 20g/L TMP to 22.3g/L BHMB and a yield of 1.10g<sub>BHMB</sub>/g<sub>TMP</sub> (100% molar yield). This work facilitates further studies of the selective oxidation on other industrially important polyols.</p>},
  author       = {Sayed, Mahmoud and Dishisha, Tarek and Sayed, Waiel F and Salem, Wesam M A and Temerk, Hanan M. and Pyo, Sang Hyun},
  issn         = {1359-5113},
  keyword      = {2,2-Bis(hydroxymethyl)butyric acid,Cell bleeding,Cell recycling,Corynebacterium sp.,Selective oxidation,Sequential batch fermentation,Trimethylolpropane},
  language     = {eng},
  month        = {08},
  publisher    = {Elsevier},
  series       = {Process Biochemistry},
  title        = {Enhanced selective oxidation of trimethylolpropane to 2,2-bis(hydroxymethyl)butyric acid using Corynebacterium sp. ATCC 21245},
  url          = {http://dx.doi.org/10.1016/j.procbio.2017.08.007},
  year         = {2017},
}