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Pellicular expanded bed matrix suitable for high flow rates

Pålsson, Eva ; Gustavsson, Per-Erik LU and Larsson, Per-Olof LU (2000) In Journal of chromatography. A 878(1). p.17-25
Abstract
A new type of expanded bed matrix with a heavy core of stainless steel covered with an agarose layer was prepared. Two bead size fractions, the smaller one (32–75 μm ∅) having a single particle core and the larger (75–180 μm ∅) with an agglomerate of stainless steel particles constituting the core, were chosen for further characterisation. The dispersion behaviour was determined both in packed bed and expanded bed modes by the retention time distribution method (RTD) and compared with the Streamline matrix (Amersham Pharmacia Biotech). The comparison turned out in favour of the new matrix. Flow rates as high as 3000 cm/h were used with the larger fraction, giving stable expanded beds with good mass transfer properties. The matrices were... (More)
A new type of expanded bed matrix with a heavy core of stainless steel covered with an agarose layer was prepared. Two bead size fractions, the smaller one (32–75 μm ∅) having a single particle core and the larger (75–180 μm ∅) with an agglomerate of stainless steel particles constituting the core, were chosen for further characterisation. The dispersion behaviour was determined both in packed bed and expanded bed modes by the retention time distribution method (RTD) and compared with the Streamline matrix (Amersham Pharmacia Biotech). The comparison turned out in favour of the new matrix. Flow rates as high as 3000 cm/h were used with the larger fraction, giving stable expanded beds with good mass transfer properties. The matrices were mechanically stable without any tendency to crack or peal, even after prolonged use. (Less)
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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Agarose coating, Stainless steel, 3000cm/h
in
Journal of chromatography. A
volume
878
issue
1
pages
9 pages
publisher
Elsevier
external identifiers
  • scopus:0342858929
ISSN
0021-9673
DOI
10.1016/S0021-9673(00)00223-5
language
English
LU publication?
yes
id
aa27c72b-11c0-4cef-aacb-dbdcc5575745
date added to LUP
2024-05-17 21:06:58
date last changed
2025-04-04 13:55:34
@article{aa27c72b-11c0-4cef-aacb-dbdcc5575745,
  abstract     = {{A new type of expanded bed matrix with a heavy core of stainless steel covered with an agarose layer was prepared. Two bead size fractions, the smaller one (32–75 μm ∅) having a single particle core and the larger (75–180 μm ∅) with an agglomerate of stainless steel particles constituting the core, were chosen for further characterisation. The dispersion behaviour was determined both in packed bed and expanded bed modes by the retention time distribution method (RTD) and compared with the Streamline matrix (Amersham Pharmacia Biotech). The comparison turned out in favour of the new matrix. Flow rates as high as 3000 cm/h were used with the larger fraction, giving stable expanded beds with good mass transfer properties. The matrices were mechanically stable without any tendency to crack or peal, even after prolonged use.}},
  author       = {{Pålsson, Eva and Gustavsson, Per-Erik and Larsson, Per-Olof}},
  issn         = {{0021-9673}},
  keywords     = {{Agarose coating; Stainless steel; 3000cm/h}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{17--25}},
  publisher    = {{Elsevier}},
  series       = {{Journal of chromatography. A}},
  title        = {{Pellicular expanded bed matrix suitable for high flow rates}},
  url          = {{http://dx.doi.org/10.1016/S0021-9673(00)00223-5}},
  doi          = {{10.1016/S0021-9673(00)00223-5}},
  volume       = {{878}},
  year         = {{2000}},
}