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A simple assay for the detection of antibodies to endocrine islet cell surface antigens

Contreas, Giovanna ; Madsen, Ole D. ; Vissing, Henrik and Lernmark, Åke LU orcid (1986) In Journal of Immunological Methods 95(1). p.135-139
Abstract

A simple and sensitive immunoradiometric assay for the detection of islet cell surface antibodies (CIRMA) has been developed. Live, transformed islet cells derived from a liver metastasis of a transplantable islet cell tumor were grown in removable microtiter wells and incubated with antibody. Cell-bound antibodies were quantitated using 125I-labelled second antibodies. The assay was used to detect islet cell antibodies present in sera from non-diabetic and diabetic BB rats and proved to be particularly effective for screening hybridoma supernatants in order to identify monoclonal antibodies recognizing islet cell surface antigens.

Please use this url to cite or link to this publication:
author
; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
(BB rat), Hybridoma screening, Islet cell surface antibody
in
Journal of Immunological Methods
volume
95
issue
1
pages
5 pages
publisher
Elsevier
external identifiers
  • pmid:3537136
  • scopus:0023001238
ISSN
0022-1759
DOI
10.1016/0022-1759(86)90328-5
language
English
LU publication?
no
id
aa2e46da-6047-4fea-9d58-9f4668a23614
date added to LUP
2019-09-16 12:32:27
date last changed
2024-03-13 08:29:48
@article{aa2e46da-6047-4fea-9d58-9f4668a23614,
  abstract     = {{<p>A simple and sensitive immunoradiometric assay for the detection of islet cell surface antibodies (CIRMA) has been developed. Live, transformed islet cells derived from a liver metastasis of a transplantable islet cell tumor were grown in removable microtiter wells and incubated with antibody. Cell-bound antibodies were quantitated using <sup>125</sup>I-labelled second antibodies. The assay was used to detect islet cell antibodies present in sera from non-diabetic and diabetic BB rats and proved to be particularly effective for screening hybridoma supernatants in order to identify monoclonal antibodies recognizing islet cell surface antigens.</p>}},
  author       = {{Contreas, Giovanna and Madsen, Ole D. and Vissing, Henrik and Lernmark, Åke}},
  issn         = {{0022-1759}},
  keywords     = {{(BB rat); Hybridoma screening; Islet cell surface antibody}},
  language     = {{eng}},
  month        = {{12}},
  number       = {{1}},
  pages        = {{135--139}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Immunological Methods}},
  title        = {{A simple assay for the detection of antibodies to endocrine islet cell surface antigens}},
  url          = {{http://dx.doi.org/10.1016/0022-1759(86)90328-5}},
  doi          = {{10.1016/0022-1759(86)90328-5}},
  volume       = {{95}},
  year         = {{1986}},
}