A simple assay for the detection of antibodies to endocrine islet cell surface antigens
(1986) In Journal of Immunological Methods 95(1). p.135-139- Abstract
A simple and sensitive immunoradiometric assay for the detection of islet cell surface antibodies (CIRMA) has been developed. Live, transformed islet cells derived from a liver metastasis of a transplantable islet cell tumor were grown in removable microtiter wells and incubated with antibody. Cell-bound antibodies were quantitated using 125I-labelled second antibodies. The assay was used to detect islet cell antibodies present in sera from non-diabetic and diabetic BB rats and proved to be particularly effective for screening hybridoma supernatants in order to identify monoclonal antibodies recognizing islet cell surface antigens.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/aa2e46da-6047-4fea-9d58-9f4668a23614
- author
- Contreas, Giovanna
; Madsen, Ole D.
; Vissing, Henrik
and Lernmark, Åke
LU
- publishing date
- 1986-12-04
- type
- Contribution to journal
- publication status
- published
- keywords
- (BB rat), Hybridoma screening, Islet cell surface antibody
- in
- Journal of Immunological Methods
- volume
- 95
- issue
- 1
- pages
- 5 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:3537136
- scopus:0023001238
- ISSN
- 0022-1759
- DOI
- 10.1016/0022-1759(86)90328-5
- language
- English
- LU publication?
- no
- id
- aa2e46da-6047-4fea-9d58-9f4668a23614
- date added to LUP
- 2019-09-16 12:32:27
- date last changed
- 2024-03-13 08:29:48
@article{aa2e46da-6047-4fea-9d58-9f4668a23614, abstract = {{<p>A simple and sensitive immunoradiometric assay for the detection of islet cell surface antibodies (CIRMA) has been developed. Live, transformed islet cells derived from a liver metastasis of a transplantable islet cell tumor were grown in removable microtiter wells and incubated with antibody. Cell-bound antibodies were quantitated using <sup>125</sup>I-labelled second antibodies. The assay was used to detect islet cell antibodies present in sera from non-diabetic and diabetic BB rats and proved to be particularly effective for screening hybridoma supernatants in order to identify monoclonal antibodies recognizing islet cell surface antigens.</p>}}, author = {{Contreas, Giovanna and Madsen, Ole D. and Vissing, Henrik and Lernmark, Åke}}, issn = {{0022-1759}}, keywords = {{(BB rat); Hybridoma screening; Islet cell surface antibody}}, language = {{eng}}, month = {{12}}, number = {{1}}, pages = {{135--139}}, publisher = {{Elsevier}}, series = {{Journal of Immunological Methods}}, title = {{A simple assay for the detection of antibodies to endocrine islet cell surface antigens}}, url = {{http://dx.doi.org/10.1016/0022-1759(86)90328-5}}, doi = {{10.1016/0022-1759(86)90328-5}}, volume = {{95}}, year = {{1986}}, }