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Mitochondrial type-I prohibitins of Arabidopsis thaliana are required for supporting proficient meristem development

Van Aken, Olivier LU ; Pecenková, Tamara; van de Cotte, Brigitte; De Rycke, Riet Maria; Eeckhout, Dominique; Fromm, Hillel; De Jaeger, Geert; Witters, Erwin; Beemster, Gerrit T S and Inzé, Dirk, et al. (2007) In Plant Journal 52(5). p.64-850
Abstract

The Arabidopsis thaliana genome expresses five evolutionarily conserved prohibitin (PHB) genes that are divided into type-I (AtPHB3 and AtPHB4) and type-II (AtPHB1, AtPHB2 and AtPHB6) classes, based on their phylogenetic relationships with yeast PHB1 and PHB2, respectively. Yeast and animal PHBs are reported to have diverse roles in the cell cycle, mitochondrial electron transport, aging and apoptosis. All transcribed Arabidopsis PHB genes are primarily expressed in both shoot and root proliferative tissues, where they are present in mitochondrial multimeric complexes. Loss of function of the type-I AtPHB4 had no phenotypic effects, while loss of function of the homologous AtPHB3 caused mitochondrial swelling, decreased meristematic... (More)

The Arabidopsis thaliana genome expresses five evolutionarily conserved prohibitin (PHB) genes that are divided into type-I (AtPHB3 and AtPHB4) and type-II (AtPHB1, AtPHB2 and AtPHB6) classes, based on their phylogenetic relationships with yeast PHB1 and PHB2, respectively. Yeast and animal PHBs are reported to have diverse roles in the cell cycle, mitochondrial electron transport, aging and apoptosis. All transcribed Arabidopsis PHB genes are primarily expressed in both shoot and root proliferative tissues, where they are present in mitochondrial multimeric complexes. Loss of function of the type-I AtPHB4 had no phenotypic effects, while loss of function of the homologous AtPHB3 caused mitochondrial swelling, decreased meristematic cell production, increased cell division time and reduced cell expansion rates, leading to severe growth retardation. Double knockout atphb3 atphb4 plants were not viable, but transgenic lines overexpressing AtPHB3 or AtPHB4 showed leaf shape aberrations and an increased shoot branching phenotype. Genome-wide microarray analysis revealed that both knockout and overexpression perturbations of AtPHB3 and AtPHB4 provoked an altered abundance of mitochondrial and stress-related transcripts. We propose that plant type-I PHBs take part in protein complexes that are necessary for proficient mitochondrial function or biogenesis, thereby supporting cell division and differentiation in apical tissues.

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published
subject
keywords
Arabidopsis, Arabidopsis Proteins, Cell Division, Cluster Analysis, Meristem, Mitochondrial Proteins, Phenotype, Phylogeny, Protein Transport, Repressor Proteins
in
Plant Journal
volume
52
issue
5
pages
15 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:36349019368
ISSN
0960-7412
DOI
10.1111/j.1365-313X.2007.03276.x
language
English
LU publication?
no
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aa45aa5a-09a1-4d6a-9f7f-26e004961b33
date added to LUP
2017-05-09 10:10:43
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2017-10-01 05:34:06
@article{aa45aa5a-09a1-4d6a-9f7f-26e004961b33,
  abstract     = {<p>The Arabidopsis thaliana genome expresses five evolutionarily conserved prohibitin (PHB) genes that are divided into type-I (AtPHB3 and AtPHB4) and type-II (AtPHB1, AtPHB2 and AtPHB6) classes, based on their phylogenetic relationships with yeast PHB1 and PHB2, respectively. Yeast and animal PHBs are reported to have diverse roles in the cell cycle, mitochondrial electron transport, aging and apoptosis. All transcribed Arabidopsis PHB genes are primarily expressed in both shoot and root proliferative tissues, where they are present in mitochondrial multimeric complexes. Loss of function of the type-I AtPHB4 had no phenotypic effects, while loss of function of the homologous AtPHB3 caused mitochondrial swelling, decreased meristematic cell production, increased cell division time and reduced cell expansion rates, leading to severe growth retardation. Double knockout atphb3 atphb4 plants were not viable, but transgenic lines overexpressing AtPHB3 or AtPHB4 showed leaf shape aberrations and an increased shoot branching phenotype. Genome-wide microarray analysis revealed that both knockout and overexpression perturbations of AtPHB3 and AtPHB4 provoked an altered abundance of mitochondrial and stress-related transcripts. We propose that plant type-I PHBs take part in protein complexes that are necessary for proficient mitochondrial function or biogenesis, thereby supporting cell division and differentiation in apical tissues.</p>},
  author       = {Van Aken, Olivier and Pecenková, Tamara and van de Cotte, Brigitte and De Rycke, Riet Maria and Eeckhout, Dominique and Fromm, Hillel and De Jaeger, Geert and Witters, Erwin and Beemster, Gerrit T S and Inzé, Dirk and Van Breusegem, Frank},
  issn         = {0960-7412},
  keyword      = {Arabidopsis,Arabidopsis Proteins,Cell Division,Cluster Analysis,Meristem,Mitochondrial Proteins,Phenotype,Phylogeny,Protein Transport,Repressor Proteins},
  language     = {eng},
  number       = {5},
  pages        = {64--850},
  publisher    = {Wiley-Blackwell},
  series       = {Plant Journal},
  title        = {Mitochondrial type-I prohibitins of Arabidopsis thaliana are required for supporting proficient meristem development},
  url          = {http://dx.doi.org/10.1111/j.1365-313X.2007.03276.x},
  volume       = {52},
  year         = {2007},
}