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Human plasma protein adsorption onto alumina nanoparticles relevant to orthopedic wear

Gibbons, John P ; Monopoli, Marco P ; Lundqvist, Martin LU ; Montes-Burgos, Iker ; McGrath, Eilis E ; Elia, Giuliano ; Lynch, Iseult LU ; Dawson, Kenneth A and Stanton, Kenneth T (2015) In Journal of Applied Biomaterials & Functional Materials 13(2). p.145-155
Abstract
Purpose
Wear of ceramic orthopedic devices generates nanoparticles in vivo that may present a different biological character from the monolithic ceramic from which they are formed. The current work investigated protein adsorption from human plasma on alumina nanoparticles and monolithic samples representative of both wear particles and the ceramic components as implanted.

Materials and Methods
A physicochemical characterization of the particles and their dispersion state was carried out, and the protein adsorption profiles were analyzed using 1D SDS-PAGE and mass spectrometry.

Results
Significant differences in protein-binding profiles were identified where the nanoparticles selectively... (More)
Purpose
Wear of ceramic orthopedic devices generates nanoparticles in vivo that may present a different biological character from the monolithic ceramic from which they are formed. The current work investigated protein adsorption from human plasma on alumina nanoparticles and monolithic samples representative of both wear particles and the ceramic components as implanted.

Materials and Methods
A physicochemical characterization of the particles and their dispersion state was carried out, and the protein adsorption profiles were analyzed using 1D SDS-PAGE and mass spectrometry.

Results
Significant differences in protein-binding profiles were identified where the nanoparticles selectively bound known transporter proteins rather than the more highly abundant serum proteins that were observed on the monoliths.

Conclusions
Proteins associated with opsonization of particles were seen to be present in the protein corona of the nanoparticles, which raises questions regarding the role of wear particles in periprosthetic tissue inflammation and aseptic loosening. (Less)
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author
; ; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Alumina, Nanoparticles, Protein Adsorption, Protein Corona
in
Journal of Applied Biomaterials & Functional Materials
volume
13
issue
2
pages
11 pages
publisher
SAGE Publications
external identifiers
  • scopus:84941207872
ISSN
2280-8000
DOI
10.5301/jabfm.5000219
language
English
LU publication?
no
id
aa50122c-d467-44ca-944b-9f57a069cf2a
date added to LUP
2021-10-19 12:51:15
date last changed
2022-03-11 20:49:07
@article{aa50122c-d467-44ca-944b-9f57a069cf2a,
  abstract     = {{<b>Purpose</b><br/>Wear of ceramic orthopedic devices generates nanoparticles in vivo that may present a different biological character from the monolithic ceramic from which they are formed. The current work investigated protein adsorption from human plasma on alumina nanoparticles and monolithic samples representative of both wear particles and the ceramic components as implanted.<br/><br/><b>Materials and Methods</b><br/>A physicochemical characterization of the particles and their dispersion state was carried out, and the protein adsorption profiles were analyzed using 1D SDS-PAGE and mass spectrometry.<br/><br/><b>Results</b><br/>Significant differences in protein-binding profiles were identified where the nanoparticles selectively bound known transporter proteins rather than the more highly abundant serum proteins that were observed on the monoliths.<br/><br/><b>Conclusions</b><br/>Proteins associated with opsonization of particles were seen to be present in the protein corona of the nanoparticles, which raises questions regarding the role of wear particles in periprosthetic tissue inflammation and aseptic loosening.}},
  author       = {{Gibbons, John P and Monopoli, Marco P and Lundqvist, Martin and Montes-Burgos, Iker and McGrath, Eilis E and Elia, Giuliano and Lynch, Iseult and Dawson, Kenneth A and Stanton, Kenneth T}},
  issn         = {{2280-8000}},
  keywords     = {{Alumina; Nanoparticles; Protein Adsorption; Protein Corona}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{145--155}},
  publisher    = {{SAGE Publications}},
  series       = {{Journal of Applied Biomaterials & Functional Materials}},
  title        = {{Human plasma protein adsorption onto alumina nanoparticles relevant to orthopedic wear}},
  url          = {{http://dx.doi.org/10.5301/jabfm.5000219}},
  doi          = {{10.5301/jabfm.5000219}},
  volume       = {{13}},
  year         = {{2015}},
}