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CD11b and CD200 on circulating monocytes differentiate two angiographic subtypes of polypoidal choroidal vasculopathy

Subhi, Yousif; Krogh Nielsen, Marie; Molbech, Christopher Rue; Oishi, Akio; Singh, Amardeep; Nissen, Mogens Holst and Sørensen, Torben Lykke (2017) In Investigative Ophthalmology and Visual Science 58(12). p.5242-5250
Abstract

Purpose: To investigate surface expression of CD11b and CD200 on circulating monocytes in patients with polypoidal choroidal vasculopathy (PCV). Methods: This was a prospective case-control study of patients with PCV (n = 27), age-matched healthy controls (n = 27), and patients with neovascular AMD (n = 49). All participants underwent a comprehensive ocular examination. Fluorescein and indocyanine green angiography were performed in patients suspected of neovascular AMD or PCV. Polypoidal choroidal vasculopathy was angiographically categorized into those with a strong presence of a branching vascular network (BVN) (type 1) or with a faint/no clear presence of a BVN (type 2). Fresh venous blood was stained with fluorescent antibodies for... (More)

Purpose: To investigate surface expression of CD11b and CD200 on circulating monocytes in patients with polypoidal choroidal vasculopathy (PCV). Methods: This was a prospective case-control study of patients with PCV (n = 27), age-matched healthy controls (n = 27), and patients with neovascular AMD (n = 49). All participants underwent a comprehensive ocular examination. Fluorescein and indocyanine green angiography were performed in patients suspected of neovascular AMD or PCV. Polypoidal choroidal vasculopathy was angiographically categorized into those with a strong presence of a branching vascular network (BVN) (type 1) or with a faint/no clear presence of a BVN (type 2). Fresh venous blood was stained with fluorescent antibodies for flow cytometric analyses. We compared the percentages of CD11b+, CD200+, and CD11b+CD200+monocytes between groups of diagnosis and between different angiographic subtypes of PCV. Results: Overall, CD11b+monocytes were both increased in patients with PCV and neovascular AMD. CD200+and CD11b+CD200+monocytes were increased in patients with neovascular AMD. An age-related increase in CD11b+CD200+monocytes was absent in patients with PCV and neovascular AMD. Patients with PCV type 1 had significantly higher CD11b+, CD200+, and CD11b+CD200+monocytes, whereas patients with PCV type 2 had levels similar to that in healthy controls. Conclusions: We found that PCV is immunologically heterogeneous with significant differences between angiographic subtypes. Increased CD11b+and CD200+monocytes in those with a strong presence of BVN indicate that BVN development may be associated with retinal injury and a VEGF-mediated process that is either reflected or propelled by systemic changes in monocytes.

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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Blood, Immunology, Microglia, Monocytes, Polypoidal choroidal vasculopathy
in
Investigative Ophthalmology and Visual Science
volume
58
issue
12
pages
9 pages
publisher
ASSOC RESEARCH VISION OPHTHALMOLOGY INC
external identifiers
  • scopus:85032155167
ISSN
0146-0404
DOI
10.1167/iovs.17-22479
language
English
LU publication?
no
id
aa7d5140-650f-45b7-b60e-07535448f046
date added to LUP
2017-11-20 12:56:16
date last changed
2018-07-08 04:28:23
@article{aa7d5140-650f-45b7-b60e-07535448f046,
  abstract     = {<p>Purpose: To investigate surface expression of CD11b and CD200 on circulating monocytes in patients with polypoidal choroidal vasculopathy (PCV). Methods: This was a prospective case-control study of patients with PCV (n = 27), age-matched healthy controls (n = 27), and patients with neovascular AMD (n = 49). All participants underwent a comprehensive ocular examination. Fluorescein and indocyanine green angiography were performed in patients suspected of neovascular AMD or PCV. Polypoidal choroidal vasculopathy was angiographically categorized into those with a strong presence of a branching vascular network (BVN) (type 1) or with a faint/no clear presence of a BVN (type 2). Fresh venous blood was stained with fluorescent antibodies for flow cytometric analyses. We compared the percentages of CD11b<sup>+</sup>, CD200<sup>+</sup>, and CD11b<sup>+</sup>CD200<sup>+</sup>monocytes between groups of diagnosis and between different angiographic subtypes of PCV. Results: Overall, CD11b<sup>+</sup>monocytes were both increased in patients with PCV and neovascular AMD. CD200<sup>+</sup>and CD11b<sup>+</sup>CD200<sup>+</sup>monocytes were increased in patients with neovascular AMD. An age-related increase in CD11b<sup>+</sup>CD200<sup>+</sup>monocytes was absent in patients with PCV and neovascular AMD. Patients with PCV type 1 had significantly higher CD11b<sup>+</sup>, CD200<sup>+</sup>, and CD11b<sup>+</sup>CD200<sup>+</sup>monocytes, whereas patients with PCV type 2 had levels similar to that in healthy controls. Conclusions: We found that PCV is immunologically heterogeneous with significant differences between angiographic subtypes. Increased CD11b<sup>+</sup>and CD200<sup>+</sup>monocytes in those with a strong presence of BVN indicate that BVN development may be associated with retinal injury and a VEGF-mediated process that is either reflected or propelled by systemic changes in monocytes.</p>},
  author       = {Subhi, Yousif and Krogh Nielsen, Marie and Molbech, Christopher Rue and Oishi, Akio and Singh, Amardeep and Nissen, Mogens Holst and Sørensen, Torben Lykke},
  issn         = {0146-0404},
  keyword      = {Blood,Immunology,Microglia,Monocytes,Polypoidal choroidal vasculopathy},
  language     = {eng},
  month        = {10},
  number       = {12},
  pages        = {5242--5250},
  publisher    = {ASSOC RESEARCH VISION OPHTHALMOLOGY INC},
  series       = {Investigative Ophthalmology and Visual Science},
  title        = {CD11b and CD200 on circulating monocytes differentiate two angiographic subtypes of polypoidal choroidal vasculopathy},
  url          = {http://dx.doi.org/10.1167/iovs.17-22479},
  volume       = {58},
  year         = {2017},
}