CD11b and CD200 on circulating monocytes differentiate two angiographic subtypes of polypoidal choroidal vasculopathy
(2017) In Investigative Ophthalmology and Visual Science 58(12). p.5242-5250- Abstract
Purpose: To investigate surface expression of CD11b and CD200 on circulating monocytes in patients with polypoidal choroidal vasculopathy (PCV). Methods: This was a prospective case-control study of patients with PCV (n = 27), age-matched healthy controls (n = 27), and patients with neovascular AMD (n = 49). All participants underwent a comprehensive ocular examination. Fluorescein and indocyanine green angiography were performed in patients suspected of neovascular AMD or PCV. Polypoidal choroidal vasculopathy was angiographically categorized into those with a strong presence of a branching vascular network (BVN) (type 1) or with a faint/no clear presence of a BVN (type 2). Fresh venous blood was stained with fluorescent antibodies for... (More)
Purpose: To investigate surface expression of CD11b and CD200 on circulating monocytes in patients with polypoidal choroidal vasculopathy (PCV). Methods: This was a prospective case-control study of patients with PCV (n = 27), age-matched healthy controls (n = 27), and patients with neovascular AMD (n = 49). All participants underwent a comprehensive ocular examination. Fluorescein and indocyanine green angiography were performed in patients suspected of neovascular AMD or PCV. Polypoidal choroidal vasculopathy was angiographically categorized into those with a strong presence of a branching vascular network (BVN) (type 1) or with a faint/no clear presence of a BVN (type 2). Fresh venous blood was stained with fluorescent antibodies for flow cytometric analyses. We compared the percentages of CD11b+, CD200+, and CD11b+CD200+monocytes between groups of diagnosis and between different angiographic subtypes of PCV. Results: Overall, CD11b+monocytes were both increased in patients with PCV and neovascular AMD. CD200+and CD11b+CD200+monocytes were increased in patients with neovascular AMD. An age-related increase in CD11b+CD200+monocytes was absent in patients with PCV and neovascular AMD. Patients with PCV type 1 had significantly higher CD11b+, CD200+, and CD11b+CD200+monocytes, whereas patients with PCV type 2 had levels similar to that in healthy controls. Conclusions: We found that PCV is immunologically heterogeneous with significant differences between angiographic subtypes. Increased CD11b+and CD200+monocytes in those with a strong presence of BVN indicate that BVN development may be associated with retinal injury and a VEGF-mediated process that is either reflected or propelled by systemic changes in monocytes.
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- author
- Subhi, Yousif ; Krogh Nielsen, Marie ; Molbech, Christopher Rue ; Oishi, Akio ; Singh, Amardeep LU ; Nissen, Mogens Holst and Sørensen, Torben Lykke
- organization
- publishing date
- 2017-10-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Blood, Immunology, Microglia, Monocytes, Polypoidal choroidal vasculopathy
- in
- Investigative Ophthalmology and Visual Science
- volume
- 58
- issue
- 12
- pages
- 9 pages
- publisher
- Association for Research in Vision and Ophthalmology Inc.
- external identifiers
-
- pmid:29049725
- scopus:85032155167
- ISSN
- 0146-0404
- DOI
- 10.1167/iovs.17-22479
- language
- English
- LU publication?
- yes
- id
- aa7d5140-650f-45b7-b60e-07535448f046
- date added to LUP
- 2017-11-20 12:56:16
- date last changed
- 2025-01-07 00:17:14
@article{aa7d5140-650f-45b7-b60e-07535448f046,
abstract = {{<p>Purpose: To investigate surface expression of CD11b and CD200 on circulating monocytes in patients with polypoidal choroidal vasculopathy (PCV). Methods: This was a prospective case-control study of patients with PCV (n = 27), age-matched healthy controls (n = 27), and patients with neovascular AMD (n = 49). All participants underwent a comprehensive ocular examination. Fluorescein and indocyanine green angiography were performed in patients suspected of neovascular AMD or PCV. Polypoidal choroidal vasculopathy was angiographically categorized into those with a strong presence of a branching vascular network (BVN) (type 1) or with a faint/no clear presence of a BVN (type 2). Fresh venous blood was stained with fluorescent antibodies for flow cytometric analyses. We compared the percentages of CD11b<sup>+</sup>, CD200<sup>+</sup>, and CD11b<sup>+</sup>CD200<sup>+</sup>monocytes between groups of diagnosis and between different angiographic subtypes of PCV. Results: Overall, CD11b<sup>+</sup>monocytes were both increased in patients with PCV and neovascular AMD. CD200<sup>+</sup>and CD11b<sup>+</sup>CD200<sup>+</sup>monocytes were increased in patients with neovascular AMD. An age-related increase in CD11b<sup>+</sup>CD200<sup>+</sup>monocytes was absent in patients with PCV and neovascular AMD. Patients with PCV type 1 had significantly higher CD11b<sup>+</sup>, CD200<sup>+</sup>, and CD11b<sup>+</sup>CD200<sup>+</sup>monocytes, whereas patients with PCV type 2 had levels similar to that in healthy controls. Conclusions: We found that PCV is immunologically heterogeneous with significant differences between angiographic subtypes. Increased CD11b<sup>+</sup>and CD200<sup>+</sup>monocytes in those with a strong presence of BVN indicate that BVN development may be associated with retinal injury and a VEGF-mediated process that is either reflected or propelled by systemic changes in monocytes.</p>}},
author = {{Subhi, Yousif and Krogh Nielsen, Marie and Molbech, Christopher Rue and Oishi, Akio and Singh, Amardeep and Nissen, Mogens Holst and Sørensen, Torben Lykke}},
issn = {{0146-0404}},
keywords = {{Blood; Immunology; Microglia; Monocytes; Polypoidal choroidal vasculopathy}},
language = {{eng}},
month = {{10}},
number = {{12}},
pages = {{5242--5250}},
publisher = {{Association for Research in Vision and Ophthalmology Inc.}},
series = {{Investigative Ophthalmology and Visual Science}},
title = {{CD11b and CD200 on circulating monocytes differentiate two angiographic subtypes of polypoidal choroidal vasculopathy}},
url = {{http://dx.doi.org/10.1167/iovs.17-22479}},
doi = {{10.1167/iovs.17-22479}},
volume = {{58}},
year = {{2017}},
}