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Systemic frequencies of T helper 1 and T helper 17 cells in patients with age-related macular degeneration : A case-control study

Singh, Amardeep LU ; Subhi, Yousif ; Krogh Nielsen, Marie ; Falk, Mads Krüger ; Matzen, Sara Maj Hyldig ; Sellebjerg, Finn and Sørensen, Torben Lykke (2017) In Scientific Reports 7. p.1-9
Abstract

Age-related macular degeneration (AMD) is a degenerative disease of the retina and a leading cause of irreversible vision loss. We investigated the systemic differences in the frequency of T helper (Th) 1 and Th17 cells in patients with non-exudative and exudative AMD and compared to age-matched controls. Flow cytometry was used to determine the systemic frequency of Th1 (CD4+CXCR3+IL12RB2+) and Th17 (CD4+CCR6+IL23R+) cells, and percentage of CD4+ T-cells expressing CXCR3, IL12RB2, CCR6, IL23R, and co-expressing CXCR3 and CCR6. The frequency of Th1 cells and CXCR3+ CD4+ T-cells was lower in patients with exudative AMD. A significant age-dependent decrement in Th1 was observed in controls, but not in non-exudative or exudative AMD. This... (More)

Age-related macular degeneration (AMD) is a degenerative disease of the retina and a leading cause of irreversible vision loss. We investigated the systemic differences in the frequency of T helper (Th) 1 and Th17 cells in patients with non-exudative and exudative AMD and compared to age-matched controls. Flow cytometry was used to determine the systemic frequency of Th1 (CD4+CXCR3+IL12RB2+) and Th17 (CD4+CCR6+IL23R+) cells, and percentage of CD4+ T-cells expressing CXCR3, IL12RB2, CCR6, IL23R, and co-expressing CXCR3 and CCR6. The frequency of Th1 cells and CXCR3+ CD4+ T-cells was lower in patients with exudative AMD. A significant age-dependent decrement in Th1 was observed in controls, but not in non-exudative or exudative AMD. This may be related to the CXCR3+ CD4+ T-cells, which showed similar pattern in controls, but not in non-exudative or exudative AMD. No significant group differences were observed for the frequency of Th17 cells. Correlation networks found several differences between controls and AMD. These data suggests the involvement of the adaptive immune system in AMD and supports the notion of AMD as a systemic disease. Our observations warrant further investigation into the role of the adaptive immune system in the pathogenesis of AMD.

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author
; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Age Factors, Aged, Aged, 80 and over, Biomarkers, Case-Control Studies, Comorbidity, Female, Flow Cytometry, Humans, Lymphocyte Count, Macular Degeneration/blood, Male, Phenotype, Risk Factors, Th1 Cells/immunology, Th17 Cells/immunology
in
Scientific Reports
volume
7
article number
605
pages
1 - 9
publisher
Nature Publishing Group
external identifiers
  • pmid:28377586
  • scopus:85017113528
ISSN
2045-2322
DOI
10.1038/s41598-017-00741-4
language
English
LU publication?
no
id
aa972a4a-e51f-49c1-84ba-6e60e2356e88
date added to LUP
2019-05-21 10:51:02
date last changed
2024-05-14 09:46:54
@article{aa972a4a-e51f-49c1-84ba-6e60e2356e88,
  abstract     = {{<p>Age-related macular degeneration (AMD) is a degenerative disease of the retina and a leading cause of irreversible vision loss. We investigated the systemic differences in the frequency of T helper (Th) 1 and Th17 cells in patients with non-exudative and exudative AMD and compared to age-matched controls. Flow cytometry was used to determine the systemic frequency of Th1 (CD4+CXCR3+IL12RB2+) and Th17 (CD4+CCR6+IL23R+) cells, and percentage of CD4+ T-cells expressing CXCR3, IL12RB2, CCR6, IL23R, and co-expressing CXCR3 and CCR6. The frequency of Th1 cells and CXCR3+ CD4+ T-cells was lower in patients with exudative AMD. A significant age-dependent decrement in Th1 was observed in controls, but not in non-exudative or exudative AMD. This may be related to the CXCR3+ CD4+ T-cells, which showed similar pattern in controls, but not in non-exudative or exudative AMD. No significant group differences were observed for the frequency of Th17 cells. Correlation networks found several differences between controls and AMD. These data suggests the involvement of the adaptive immune system in AMD and supports the notion of AMD as a systemic disease. Our observations warrant further investigation into the role of the adaptive immune system in the pathogenesis of AMD.</p>}},
  author       = {{Singh, Amardeep and Subhi, Yousif and Krogh Nielsen, Marie and Falk, Mads Krüger and Matzen, Sara Maj Hyldig and Sellebjerg, Finn and Sørensen, Torben Lykke}},
  issn         = {{2045-2322}},
  keywords     = {{Age Factors; Aged; Aged, 80 and over; Biomarkers; Case-Control Studies; Comorbidity; Female; Flow Cytometry; Humans; Lymphocyte Count; Macular Degeneration/blood; Male; Phenotype; Risk Factors; Th1 Cells/immunology; Th17 Cells/immunology}},
  language     = {{eng}},
  month        = {{04}},
  pages        = {{1--9}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Scientific Reports}},
  title        = {{Systemic frequencies of T helper 1 and T helper 17 cells in patients with age-related macular degeneration : A case-control study}},
  url          = {{http://dx.doi.org/10.1038/s41598-017-00741-4}},
  doi          = {{10.1038/s41598-017-00741-4}},
  volume       = {{7}},
  year         = {{2017}},
}