Analysis of "old" proteins unmasks dynamic gradient of cartilage turnover in human limbs
(2019) In Science Advances 5(10).- Abstract
Unlike highly regenerative animals, such as axolotls, humans are believed to be unable to counteract cumulative damage, such as repetitive joint use and injury that lead to the breakdown of cartilage and the development of osteoarthritis. Turnover of insoluble collagen has been suggested to be very limited in human adult cartilage. The goal of this study was to explore protein turnover in articular cartilage from human lower limb joints. Analyzing molecular clocks in the form of nonenzymatically deamidated proteins, we unmasked a position-dependent gradient (distal high, proximal low) of protein turnover, indicative of a gradient of tissue anabolism reflecting innate tissue repair capacity in human lower limb cartilages that is... (More)
Unlike highly regenerative animals, such as axolotls, humans are believed to be unable to counteract cumulative damage, such as repetitive joint use and injury that lead to the breakdown of cartilage and the development of osteoarthritis. Turnover of insoluble collagen has been suggested to be very limited in human adult cartilage. The goal of this study was to explore protein turnover in articular cartilage from human lower limb joints. Analyzing molecular clocks in the form of nonenzymatically deamidated proteins, we unmasked a position-dependent gradient (distal high, proximal low) of protein turnover, indicative of a gradient of tissue anabolism reflecting innate tissue repair capacity in human lower limb cartilages that is associated with expression of limb-regenerative microRNAs. This association shows a potential link to a capacity, albeit limited, for regeneration that might be exploited to enhance joint repair and establish a basis for human limb regeneration.
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- author
- Hsueh, Ming-Feng ; Önnerfjord, Patrik LU ; Bolognesi, Michael P ; Easley, Mark E and Kraus, Virginia B
- organization
- publishing date
- 2019-10
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cartilage Oligomeric Matrix Protein/metabolism, Cartilage, Articular/metabolism, Collagen/metabolism, Databases, Factual, Extremities/physiology, Fibronectins/metabolism, Half-Life, Humans, Mass Spectrometry, MicroRNAs/metabolism, Osteoarthritis/metabolism, Proteome/analysis, Regeneration
- in
- Science Advances
- volume
- 5
- issue
- 10
- article number
- eaax3203
- pages
- 9 pages
- publisher
- American Association for the Advancement of Science (AAAS)
- external identifiers
-
- pmid:31633025
- scopus:85073621181
- ISSN
- 2375-2548
- DOI
- 10.1126/sciadv.aax3203
- language
- English
- LU publication?
- yes
- additional info
- Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
- id
- aad1485d-05c3-4c39-be6a-e269c5055756
- date added to LUP
- 2022-01-13 10:29:46
- date last changed
- 2024-09-22 08:56:01
@article{aad1485d-05c3-4c39-be6a-e269c5055756, abstract = {{<p>Unlike highly regenerative animals, such as axolotls, humans are believed to be unable to counteract cumulative damage, such as repetitive joint use and injury that lead to the breakdown of cartilage and the development of osteoarthritis. Turnover of insoluble collagen has been suggested to be very limited in human adult cartilage. The goal of this study was to explore protein turnover in articular cartilage from human lower limb joints. Analyzing molecular clocks in the form of nonenzymatically deamidated proteins, we unmasked a position-dependent gradient (distal high, proximal low) of protein turnover, indicative of a gradient of tissue anabolism reflecting innate tissue repair capacity in human lower limb cartilages that is associated with expression of limb-regenerative microRNAs. This association shows a potential link to a capacity, albeit limited, for regeneration that might be exploited to enhance joint repair and establish a basis for human limb regeneration.</p>}}, author = {{Hsueh, Ming-Feng and Önnerfjord, Patrik and Bolognesi, Michael P and Easley, Mark E and Kraus, Virginia B}}, issn = {{2375-2548}}, keywords = {{Cartilage Oligomeric Matrix Protein/metabolism; Cartilage, Articular/metabolism; Collagen/metabolism; Databases, Factual; Extremities/physiology; Fibronectins/metabolism; Half-Life; Humans; Mass Spectrometry; MicroRNAs/metabolism; Osteoarthritis/metabolism; Proteome/analysis; Regeneration}}, language = {{eng}}, number = {{10}}, publisher = {{American Association for the Advancement of Science (AAAS)}}, series = {{Science Advances}}, title = {{Analysis of "old" proteins unmasks dynamic gradient of cartilage turnover in human limbs}}, url = {{http://dx.doi.org/10.1126/sciadv.aax3203}}, doi = {{10.1126/sciadv.aax3203}}, volume = {{5}}, year = {{2019}}, }