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Analysis of "old" proteins unmasks dynamic gradient of cartilage turnover in human limbs

Hsueh, Ming-Feng ; Önnerfjord, Patrik LU orcid ; Bolognesi, Michael P ; Easley, Mark E and Kraus, Virginia B (2019) In Science Advances 5(10).
Abstract

Unlike highly regenerative animals, such as axolotls, humans are believed to be unable to counteract cumulative damage, such as repetitive joint use and injury that lead to the breakdown of cartilage and the development of osteoarthritis. Turnover of insoluble collagen has been suggested to be very limited in human adult cartilage. The goal of this study was to explore protein turnover in articular cartilage from human lower limb joints. Analyzing molecular clocks in the form of nonenzymatically deamidated proteins, we unmasked a position-dependent gradient (distal high, proximal low) of protein turnover, indicative of a gradient of tissue anabolism reflecting innate tissue repair capacity in human lower limb cartilages that is... (More)

Unlike highly regenerative animals, such as axolotls, humans are believed to be unable to counteract cumulative damage, such as repetitive joint use and injury that lead to the breakdown of cartilage and the development of osteoarthritis. Turnover of insoluble collagen has been suggested to be very limited in human adult cartilage. The goal of this study was to explore protein turnover in articular cartilage from human lower limb joints. Analyzing molecular clocks in the form of nonenzymatically deamidated proteins, we unmasked a position-dependent gradient (distal high, proximal low) of protein turnover, indicative of a gradient of tissue anabolism reflecting innate tissue repair capacity in human lower limb cartilages that is associated with expression of limb-regenerative microRNAs. This association shows a potential link to a capacity, albeit limited, for regeneration that might be exploited to enhance joint repair and establish a basis for human limb regeneration.

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cartilage Oligomeric Matrix Protein/metabolism, Cartilage, Articular/metabolism, Collagen/metabolism, Databases, Factual, Extremities/physiology, Fibronectins/metabolism, Half-Life, Humans, Mass Spectrometry, MicroRNAs/metabolism, Osteoarthritis/metabolism, Proteome/analysis, Regeneration
in
Science Advances
volume
5
issue
10
article number
eaax3203
pages
9 pages
publisher
American Association for the Advancement of Science (AAAS)
external identifiers
  • pmid:31633025
  • scopus:85073621181
ISSN
2375-2548
DOI
10.1126/sciadv.aax3203
language
English
LU publication?
yes
additional info
Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
id
aad1485d-05c3-4c39-be6a-e269c5055756
date added to LUP
2022-01-13 10:29:46
date last changed
2024-04-06 16:29:18
@article{aad1485d-05c3-4c39-be6a-e269c5055756,
  abstract     = {{<p>Unlike highly regenerative animals, such as axolotls, humans are believed to be unable to counteract cumulative damage, such as repetitive joint use and injury that lead to the breakdown of cartilage and the development of osteoarthritis. Turnover of insoluble collagen has been suggested to be very limited in human adult cartilage. The goal of this study was to explore protein turnover in articular cartilage from human lower limb joints. Analyzing molecular clocks in the form of nonenzymatically deamidated proteins, we unmasked a position-dependent gradient (distal high, proximal low) of protein turnover, indicative of a gradient of tissue anabolism reflecting innate tissue repair capacity in human lower limb cartilages that is associated with expression of limb-regenerative microRNAs. This association shows a potential link to a capacity, albeit limited, for regeneration that might be exploited to enhance joint repair and establish a basis for human limb regeneration.</p>}},
  author       = {{Hsueh, Ming-Feng and Önnerfjord, Patrik and Bolognesi, Michael P and Easley, Mark E and Kraus, Virginia B}},
  issn         = {{2375-2548}},
  keywords     = {{Cartilage Oligomeric Matrix Protein/metabolism; Cartilage, Articular/metabolism; Collagen/metabolism; Databases, Factual; Extremities/physiology; Fibronectins/metabolism; Half-Life; Humans; Mass Spectrometry; MicroRNAs/metabolism; Osteoarthritis/metabolism; Proteome/analysis; Regeneration}},
  language     = {{eng}},
  number       = {{10}},
  publisher    = {{American Association for the Advancement of Science (AAAS)}},
  series       = {{Science Advances}},
  title        = {{Analysis of "old" proteins unmasks dynamic gradient of cartilage turnover in human limbs}},
  url          = {{http://dx.doi.org/10.1126/sciadv.aax3203}},
  doi          = {{10.1126/sciadv.aax3203}},
  volume       = {{5}},
  year         = {{2019}},
}