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In vitro differentiation of human pluripotent stem cells into the B lineage using OP9-MS5 co-culture

Richardson, Simon E. ; Ghazanfari, Roshanak LU ; Chhetri, Jyoti ; Enver, Tariq LU and Böiers, Charlotta LU (2021) In STAR Protocols 2(2).
Abstract

In vitro differentiation of human pluripotent stem cells (hPSCs) offers a genetically tractable system to examine the physiology and pathology of human tissue development and differentiation. We have used this approach to model the earliest stages of human B lineage development and characterize potential target cells for the in utero initiation of childhood B acute lymphoblastic leukemia. Herein, we detail critical aspects of the protocol including reagent validation, controls, and examples of surface markers used for analysis and cell sorting. For complete details on the use and execution of this protocol, please refer to Boiers et al. (2018).

Please use this url to cite or link to this publication:
author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cell culture, Cell Differentiation, Stem Cells
in
STAR Protocols
volume
2
issue
2
article number
100420
publisher
Cell Press
external identifiers
  • pmid:33899010
  • scopus:85108152192
ISSN
2666-1667
DOI
10.1016/j.xpro.2021.100420
language
English
LU publication?
yes
id
aae050dc-361a-46be-aa21-92a5680c31d8
date added to LUP
2021-07-15 11:34:06
date last changed
2024-06-15 13:28:49
@article{aae050dc-361a-46be-aa21-92a5680c31d8,
  abstract     = {{<p>In vitro differentiation of human pluripotent stem cells (hPSCs) offers a genetically tractable system to examine the physiology and pathology of human tissue development and differentiation. We have used this approach to model the earliest stages of human B lineage development and characterize potential target cells for the in utero initiation of childhood B acute lymphoblastic leukemia. Herein, we detail critical aspects of the protocol including reagent validation, controls, and examples of surface markers used for analysis and cell sorting. For complete details on the use and execution of this protocol, please refer to Boiers et al. (2018).</p>}},
  author       = {{Richardson, Simon E. and Ghazanfari, Roshanak and Chhetri, Jyoti and Enver, Tariq and Böiers, Charlotta}},
  issn         = {{2666-1667}},
  keywords     = {{Cell culture; Cell Differentiation; Stem Cells}},
  language     = {{eng}},
  number       = {{2}},
  publisher    = {{Cell Press}},
  series       = {{STAR Protocols}},
  title        = {{In vitro differentiation of human pluripotent stem cells into the B lineage using OP9-MS5 co-culture}},
  url          = {{http://dx.doi.org/10.1016/j.xpro.2021.100420}},
  doi          = {{10.1016/j.xpro.2021.100420}},
  volume       = {{2}},
  year         = {{2021}},
}