In vitro differentiation of human pluripotent stem cells into the B lineage using OP9-MS5 co-culture
(2021) In STAR Protocols 2(2).- Abstract
In vitro differentiation of human pluripotent stem cells (hPSCs) offers a genetically tractable system to examine the physiology and pathology of human tissue development and differentiation. We have used this approach to model the earliest stages of human B lineage development and characterize potential target cells for the in utero initiation of childhood B acute lymphoblastic leukemia. Herein, we detail critical aspects of the protocol including reagent validation, controls, and examples of surface markers used for analysis and cell sorting. For complete details on the use and execution of this protocol, please refer to Boiers et al. (2018).
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/aae050dc-361a-46be-aa21-92a5680c31d8
- author
- Richardson, Simon E. ; Ghazanfari, Roshanak LU ; Chhetri, Jyoti ; Enver, Tariq LU and Böiers, Charlotta LU
- organization
- publishing date
- 2021
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cell culture, Cell Differentiation, Stem Cells
- in
- STAR Protocols
- volume
- 2
- issue
- 2
- article number
- 100420
- publisher
- Cell Press
- external identifiers
-
- pmid:33899010
- scopus:85108152192
- ISSN
- 2666-1667
- DOI
- 10.1016/j.xpro.2021.100420
- language
- English
- LU publication?
- yes
- id
- aae050dc-361a-46be-aa21-92a5680c31d8
- date added to LUP
- 2021-07-15 11:34:06
- date last changed
- 2024-09-07 21:31:51
@article{aae050dc-361a-46be-aa21-92a5680c31d8, abstract = {{<p>In vitro differentiation of human pluripotent stem cells (hPSCs) offers a genetically tractable system to examine the physiology and pathology of human tissue development and differentiation. We have used this approach to model the earliest stages of human B lineage development and characterize potential target cells for the in utero initiation of childhood B acute lymphoblastic leukemia. Herein, we detail critical aspects of the protocol including reagent validation, controls, and examples of surface markers used for analysis and cell sorting. For complete details on the use and execution of this protocol, please refer to Boiers et al. (2018).</p>}}, author = {{Richardson, Simon E. and Ghazanfari, Roshanak and Chhetri, Jyoti and Enver, Tariq and Böiers, Charlotta}}, issn = {{2666-1667}}, keywords = {{Cell culture; Cell Differentiation; Stem Cells}}, language = {{eng}}, number = {{2}}, publisher = {{Cell Press}}, series = {{STAR Protocols}}, title = {{In vitro differentiation of human pluripotent stem cells into the B lineage using OP9-MS5 co-culture}}, url = {{http://dx.doi.org/10.1016/j.xpro.2021.100420}}, doi = {{10.1016/j.xpro.2021.100420}}, volume = {{2}}, year = {{2021}}, }