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Heterokaryon-based reprogramming for pluripotency

Pereira, Carlos Filipe LU and Fisher, Amanda G. (2009) In Current Protocols in Stem Cell Biology SUPPL 9. p.1-14
Abstract

Embryonic stem (ES) cells have the ability to self-renew, execute multiple lineage paths, and dominantly reprogram differentiated cells upon cell fusion. Here, we describe an approach that reprograms human B lymphocytes toward pluripotency by generating inter-species heterokaryons with mouse ES cells. This induces a human ES-specific gene expression profile, in which the extent and the rapidity of conversion allows us to compare the capacity of different mouse ES cell lines to dominantly induce pluripotency. This approach, coupled with pharmacological inhibition, gene knock-out, or knockdown permits factors that are required to directly induce reprogramming to be defined individually, as well as in combination. Experimental... (More)

Embryonic stem (ES) cells have the ability to self-renew, execute multiple lineage paths, and dominantly reprogram differentiated cells upon cell fusion. Here, we describe an approach that reprograms human B lymphocytes toward pluripotency by generating inter-species heterokaryons with mouse ES cells. This induces a human ES-specific gene expression profile, in which the extent and the rapidity of conversion allows us to compare the capacity of different mouse ES cell lines to dominantly induce pluripotency. This approach, coupled with pharmacological inhibition, gene knock-out, or knockdown permits factors that are required to directly induce reprogramming to be defined individually, as well as in combination. Experimental heterokaryons provide a simple and tractable approach to address the mechanisms underlying direct reprogramming to pluripotency. The procedure requires 5 days to complete.

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Please use this url to cite or link to this publication:
author
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
keywords
Cell fusion, Embryonic stem (ES) cell, Heterokaryon, Pluripotency, Reprogramming
in
Current Protocols in Stem Cell Biology
volume
SUPPL 9
pages
4 pages
publisher
Wiley Online Library
external identifiers
  • scopus:65149087887
ISBN
9780470151808
DOI
10.1002/9780470151808.sc04b01s9
language
English
LU publication?
no
id
ab65c501-15dc-4a21-8115-ba6bba881a8b
date added to LUP
2017-10-02 17:31:13
date last changed
2017-10-15 04:55:59
@inbook{ab65c501-15dc-4a21-8115-ba6bba881a8b,
  abstract     = {<p>Embryonic stem (ES) cells have the ability to self-renew, execute multiple lineage paths, and dominantly reprogram differentiated cells upon cell fusion. Here, we describe an approach that reprograms human B lymphocytes toward pluripotency by generating inter-species heterokaryons with mouse ES cells. This induces a human ES-specific gene expression profile, in which the extent and the rapidity of conversion allows us to compare the capacity of different mouse ES cell lines to dominantly induce pluripotency. This approach, coupled with pharmacological inhibition, gene knock-out, or knockdown permits factors that are required to directly induce reprogramming to be defined individually, as well as in combination. Experimental heterokaryons provide a simple and tractable approach to address the mechanisms underlying direct reprogramming to pluripotency. The procedure requires 5 days to complete.</p>},
  author       = {Pereira, Carlos Filipe and Fisher, Amanda G.},
  isbn         = {9780470151808},
  keyword      = {Cell fusion,Embryonic stem (ES) cell,Heterokaryon,Pluripotency,Reprogramming},
  language     = {eng},
  pages        = {1--14},
  publisher    = {Wiley Online Library},
  series       = {Current Protocols in Stem Cell Biology},
  title        = {Heterokaryon-based reprogramming for pluripotency},
  url          = {http://dx.doi.org/10.1002/9780470151808.sc04b01s9},
  volume       = {SUPPL 9},
  year         = {2009},
}