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Initial seeding of the embryonic thymus by immune-restricted lympho-myeloid progenitors

Luis, Tiago C.; Luc, Sidinh LU ; Mizukami, Takuo; Boukarabila, Hanane; Thongjuea, Supat; Woll, Petter S.; Azzoni, Emanuele; Giustacchini, Alice; Lutteropp, Michael and Bouriez-Jones, Tiphaine, et al. (2016) In Nature Immunology 17(12). p.1424-1435
Abstract

The final stages of restriction to the T cell lineage occur in the thymus after the entry of thymus-seeding progenitors (TSPs). The identity and lineage potential of TSPs remains unclear. Because the first embryonic TSPs enter a non-vascularized thymic rudiment, we were able to directly image and establish the functional and molecular properties of embryonic thymopoiesis-initiating progenitors (T-IPs) before their entry into the thymus and activation of Notch signaling. T-IPs did not include multipotent stem cells or molecular evidence of T cell-restricted progenitors. Instead, single-cell molecular and functional analysis demonstrated that most fetal T-IPs expressed genes of and had the potential to develop into lymphoid as well as... (More)

The final stages of restriction to the T cell lineage occur in the thymus after the entry of thymus-seeding progenitors (TSPs). The identity and lineage potential of TSPs remains unclear. Because the first embryonic TSPs enter a non-vascularized thymic rudiment, we were able to directly image and establish the functional and molecular properties of embryonic thymopoiesis-initiating progenitors (T-IPs) before their entry into the thymus and activation of Notch signaling. T-IPs did not include multipotent stem cells or molecular evidence of T cell-restricted progenitors. Instead, single-cell molecular and functional analysis demonstrated that most fetal T-IPs expressed genes of and had the potential to develop into lymphoid as well as myeloid components of the immune system. Moreover, studies of embryos deficient in the transcriptional regulator RBPJ demonstrated that canonical Notch signaling was not involved in pre-thymic restriction to the T cell lineage or the migration of T-IPs.

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Nature Immunology
volume
17
issue
12
pages
12 pages
publisher
Nature Publishing Group
external identifiers
  • scopus:84989884306
  • wos:000388056400013
ISSN
1529-2908
DOI
10.1038/ni.3576
language
English
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yes
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ab7be965-b85d-46a5-b2af-2d6d1656fe2a
date added to LUP
2016-12-07 08:17:53
date last changed
2017-05-29 13:56:54
@article{ab7be965-b85d-46a5-b2af-2d6d1656fe2a,
  abstract     = {<p>The final stages of restriction to the T cell lineage occur in the thymus after the entry of thymus-seeding progenitors (TSPs). The identity and lineage potential of TSPs remains unclear. Because the first embryonic TSPs enter a non-vascularized thymic rudiment, we were able to directly image and establish the functional and molecular properties of embryonic thymopoiesis-initiating progenitors (T-IPs) before their entry into the thymus and activation of Notch signaling. T-IPs did not include multipotent stem cells or molecular evidence of T cell-restricted progenitors. Instead, single-cell molecular and functional analysis demonstrated that most fetal T-IPs expressed genes of and had the potential to develop into lymphoid as well as myeloid components of the immune system. Moreover, studies of embryos deficient in the transcriptional regulator RBPJ demonstrated that canonical Notch signaling was not involved in pre-thymic restriction to the T cell lineage or the migration of T-IPs.</p>},
  author       = {Luis, Tiago C. and Luc, Sidinh and Mizukami, Takuo and Boukarabila, Hanane and Thongjuea, Supat and Woll, Petter S. and Azzoni, Emanuele and Giustacchini, Alice and Lutteropp, Michael and Bouriez-Jones, Tiphaine and Vaidya, Harsh and Mead, Adam J. and Atkinson, Deborah and Böiers, Charlotta and Carrelha, Joana and MacAulay, Iain C. and Patient, Roger and Geissmann, Frederic and Nerlov, Claus and Sandberg, Rickard and De Bruijn, Marella F T R and Blackburn, C. Clare and Godin, Isabelle and Jacobsen, Sten Eirik W},
  issn         = {1529-2908},
  language     = {eng},
  month        = {12},
  number       = {12},
  pages        = {1424--1435},
  publisher    = {Nature Publishing Group},
  series       = {Nature Immunology},
  title        = {Initial seeding of the embryonic thymus by immune-restricted lympho-myeloid progenitors},
  url          = {http://dx.doi.org/10.1038/ni.3576},
  volume       = {17},
  year         = {2016},
}