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FSP27 and PLIN1 interaction promotes the formation of large lipid droplets in human adipocytes.

Tan Grahn, Hooi Min LU ; Zhang, Yan; Lee, Mi-Jeong; Sommer, Andreia Gianotti; Mostoslavsky, Gustavo; Fried, Susan K; Greenberg, Andrew S and Puri, Vishwajeet (2013) In Biochemical and Biophysical Research Communications 432(2). p.296-301
Abstract
Human adipocytes express high levels of two distinct lipid droplet proteins, fat specific protein 27 (FSP27; also called CIDEC), a member of the CIDE family, and perilipin1 (PLIN1), a member of the PAT family. Both proteins play a role in fat metabolism in adipocytes, but how they interact is not known. Our present study demonstrates that FSP27 and PLIN1 co-localize and interact in cultured human primary adipocytes. We also found that the C-terminal domain of FSP27, aa 120-220, interacts with PLIN1. Individual expression of exogenous FSP27 or PLIN1 increased triglyceride content and decreased glycerol release (a measure of lipolysis), but co-expression of both proteins did not further increase triglyceride content or decrease lipolysis in... (More)
Human adipocytes express high levels of two distinct lipid droplet proteins, fat specific protein 27 (FSP27; also called CIDEC), a member of the CIDE family, and perilipin1 (PLIN1), a member of the PAT family. Both proteins play a role in fat metabolism in adipocytes, but how they interact is not known. Our present study demonstrates that FSP27 and PLIN1 co-localize and interact in cultured human primary adipocytes. We also found that the C-terminal domain of FSP27, aa 120-220, interacts with PLIN1. Individual expression of exogenous FSP27 or PLIN1 increased triglyceride content and decreased glycerol release (a measure of lipolysis), but co-expression of both proteins did not further increase triglyceride content or decrease lipolysis in human adipocytes. However, the combination of PLIN1 and FSP27 increased the average size of lipid droplets or caused the formation of unilocular adipocytes. Our data suggest that FSP27 interacts with PLIN1 to regulate lipid droplet size in human adipocytes in a concerted manner. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Adipocytes: metabolism, Carrier Proteins: metabolism, Phosphoproteins: metabolism, Proteins: genetics, Proteins: metabolism, Triglycerides: metabolism
in
Biochemical and Biophysical Research Communications
volume
432
issue
2
pages
296 - 301
publisher
Elsevier
external identifiers
  • pmid:23399566
  • scopus:84875254733
ISSN
1090-2104
DOI
10.1016/j.bbrc.2013.01.113
language
English
LU publication?
no
id
ab9cde10-bb8e-4a9c-8578-3f1342bbb2b0 (old id 5425027)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/23399566?dopt=Abstract
date added to LUP
2015-05-21 07:39:52
date last changed
2019-04-10 02:08:53
@article{ab9cde10-bb8e-4a9c-8578-3f1342bbb2b0,
  abstract     = {Human adipocytes express high levels of two distinct lipid droplet proteins, fat specific protein 27 (FSP27; also called CIDEC), a member of the CIDE family, and perilipin1 (PLIN1), a member of the PAT family. Both proteins play a role in fat metabolism in adipocytes, but how they interact is not known. Our present study demonstrates that FSP27 and PLIN1 co-localize and interact in cultured human primary adipocytes. We also found that the C-terminal domain of FSP27, aa 120-220, interacts with PLIN1. Individual expression of exogenous FSP27 or PLIN1 increased triglyceride content and decreased glycerol release (a measure of lipolysis), but co-expression of both proteins did not further increase triglyceride content or decrease lipolysis in human adipocytes. However, the combination of PLIN1 and FSP27 increased the average size of lipid droplets or caused the formation of unilocular adipocytes. Our data suggest that FSP27 interacts with PLIN1 to regulate lipid droplet size in human adipocytes in a concerted manner.},
  author       = {Tan Grahn, Hooi Min and Zhang, Yan and Lee, Mi-Jeong and Sommer, Andreia Gianotti and Mostoslavsky, Gustavo and Fried, Susan K and Greenberg, Andrew S and Puri, Vishwajeet},
  issn         = {1090-2104},
  keyword      = {Adipocytes: metabolism,Carrier Proteins: metabolism,Phosphoproteins: metabolism,Proteins: genetics,Proteins: metabolism,Triglycerides: metabolism},
  language     = {eng},
  number       = {2},
  pages        = {296--301},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {FSP27 and PLIN1 interaction promotes the formation of large lipid droplets in human adipocytes.},
  url          = {http://dx.doi.org/10.1016/j.bbrc.2013.01.113},
  volume       = {432},
  year         = {2013},
}