Embedded Ultrathin Cluster Electrodes for Long-Term Recordings in Deep Brain Centers
Etemadi, Leila LU ; Mohammed, Mohsin LU ; Thorbergsson, Palmi Thor LU ; Ekstrand, Joakim LU ; Friberg, Annika LU ; Granmo, Marcus LU ; Pettersson, Lina M E LU and Schouenborg, Jens LU
(2016)
In PLoS ONE
11(5).
- Abstract
Neural interfaces which allow long-term recordings in deep brain structures in awake freely moving animals have the potential of becoming highly valuable tools in neuroscience. However, the recording quality usually deteriorates over time, probably at least partly due to tissue reactions caused by injuries during implantation, and subsequently micro-forces due to a lack of mechanical compliance between the tissue and neural interface. To address this challenge, we developed a gelatin embedded neural interface comprising highly flexible electrodes and evaluated its long term recording properties. Bundles of ultrathin parylene C coated platinum electrodes (N = 29) were embedded in a hard gelatin based matrix shaped like a needle, and... (More)
Neural interfaces which allow long-term recordings in deep brain structures in awake freely moving animals have the potential of becoming highly valuable tools in neuroscience. However, the recording quality usually deteriorates over time, probably at least partly due to tissue reactions caused by injuries during implantation, and subsequently micro-forces due to a lack of mechanical compliance between the tissue and neural interface. To address this challenge, we developed a gelatin embedded neural interface comprising highly flexible electrodes and evaluated its long term recording properties. Bundles of ultrathin parylene C coated platinum electrodes (N = 29) were embedded in a hard gelatin based matrix shaped like a needle, and coated with Kollicoat™ to retard dissolution of gelatin during the implantation. The implantation parameters were established in an in vitro model of the brain (0.5% agarose). Following a craniotomy in the anesthetized rat, the gelatin embedded electrodes were stereotactically inserted to a pre-target position, and after gelatin dissolution the electrodes were further advanced and spread out in the area of the subthalamic nucleus (STN). The performance of the implanted electrodes was evaluated under anesthesia, during 8 weeks. Apart from an increase in the median-noise level during the first 4 weeks, the electrode impedance and signal-to-noise ratio of single-units remained stable throughout the experiment. Histological postmortem analysis confirmed implantation in the area of STN in most animals. In conclusion, by combining novel biocompatible implantation techniques and ultra-flexible electrodes, long-term neuronal recordings from deep brain structures with no significant deterioration of electrode function were achieved.
(Less)
- author
- Etemadi, Leila
LU
; Mohammed, Mohsin
LU
; Thorbergsson, Palmi Thor
LU
; Ekstrand, Joakim
LU
; Friberg, Annika
LU
; Granmo, Marcus
LU
; Pettersson, Lina M E
LU
and Schouenborg, Jens
LU
- organization
- publishing date
- 2016
- type
- Contribution to journal
- publication status
- published
- subject
- in
- PLoS ONE
- volume
- 11
- issue
- 5
- article number
- e0155109
- publisher
- Public Library of Science (PLoS)
- external identifiers
-
- pmid:27159159
- scopus:84968718695
- wos:000376576700045
- ISSN
- 1932-6203
- DOI
- 10.1371/journal.pone.0155109
- language
- English
- LU publication?
- yes
- id
- aba306ad-f21e-444e-94d6-c919600893b1
- date added to LUP
- 2016-05-16 12:43:57
- date last changed
- 2024-02-02 14:48:09
@article{aba306ad-f21e-444e-94d6-c919600893b1,
abstract = {{<p>Neural interfaces which allow long-term recordings in deep brain structures in awake freely moving animals have the potential of becoming highly valuable tools in neuroscience. However, the recording quality usually deteriorates over time, probably at least partly due to tissue reactions caused by injuries during implantation, and subsequently micro-forces due to a lack of mechanical compliance between the tissue and neural interface. To address this challenge, we developed a gelatin embedded neural interface comprising highly flexible electrodes and evaluated its long term recording properties. Bundles of ultrathin parylene C coated platinum electrodes (N = 29) were embedded in a hard gelatin based matrix shaped like a needle, and coated with Kollicoat™ to retard dissolution of gelatin during the implantation. The implantation parameters were established in an in vitro model of the brain (0.5% agarose). Following a craniotomy in the anesthetized rat, the gelatin embedded electrodes were stereotactically inserted to a pre-target position, and after gelatin dissolution the electrodes were further advanced and spread out in the area of the subthalamic nucleus (STN). The performance of the implanted electrodes was evaluated under anesthesia, during 8 weeks. Apart from an increase in the median-noise level during the first 4 weeks, the electrode impedance and signal-to-noise ratio of single-units remained stable throughout the experiment. Histological postmortem analysis confirmed implantation in the area of STN in most animals. In conclusion, by combining novel biocompatible implantation techniques and ultra-flexible electrodes, long-term neuronal recordings from deep brain structures with no significant deterioration of electrode function were achieved.</p>}},
author = {{Etemadi, Leila and Mohammed, Mohsin and Thorbergsson, Palmi Thor and Ekstrand, Joakim and Friberg, Annika and Granmo, Marcus and Pettersson, Lina M E and Schouenborg, Jens}},
issn = {{1932-6203}},
language = {{eng}},
number = {{5}},
publisher = {{Public Library of Science (PLoS)}},
series = {{PLoS ONE}},
title = {{Embedded Ultrathin Cluster Electrodes for Long-Term Recordings in Deep Brain Centers}},
url = {{http://dx.doi.org/10.1371/journal.pone.0155109}},
doi = {{10.1371/journal.pone.0155109}},
volume = {{11}},
year = {{2016}},
}