Quantification of human cytomegalovirus DNA by a new capture hybrid polymerase chain reaction enzyme-linked immunosorbent assay in plasma and peripheral blood mononuclear cells of bone marrow transplant recipients
(2008) In Experimental and clinical transplantation 6(4). p.294-300- Abstract
OBJECTIVES: Quantitative monitoring of human cytomegalovirus infections is helpful in determining appropriate antiviral management in patients who receive bone marrow transplants. We sought to design and evaluate a new cytomegalovirus capture hybrid polymerase chain reaction enzyme-linked immunosorbent assay (PCR-ELISA) in plasma and peripheral blood mononuclear cells to monitor cytomegalovirus infection in bone marrow transplant recipients.
PATIENTS AND METHODS: Twenty-six patients who received allogeneic bone marrow transplants, including 17 male patients and 9 female patients (9 adults, 17 children), were enrolled in this study. A total of 313 consecutive whole blood specimens, before and from 7 to 120 days after transplant,... (More)
OBJECTIVES: Quantitative monitoring of human cytomegalovirus infections is helpful in determining appropriate antiviral management in patients who receive bone marrow transplants. We sought to design and evaluate a new cytomegalovirus capture hybrid polymerase chain reaction enzyme-linked immunosorbent assay (PCR-ELISA) in plasma and peripheral blood mononuclear cells to monitor cytomegalovirus infection in bone marrow transplant recipients.
PATIENTS AND METHODS: Twenty-six patients who received allogeneic bone marrow transplants, including 17 male patients and 9 female patients (9 adults, 17 children), were enrolled in this study. A total of 313 consecutive whole blood specimens, before and from 7 to 120 days after transplant, was evaluated in the study. A newly designed biotinylated probe-mediated quantitative competitive PCR-ELISA test was used to determine cytomegalovirus load in specimens of peripheral blood mononuclear cells and plasma.
RESULTS: All 26 patients were cytomegalovirus seropositive before transplant. Capture hybrid PCR-ELISA of peripheral blood mononuclear cells detected cytomegalovirus DNA in 287 of 313 specimens (91.7%) even in cases with no active cytomegalovirus infection. In plasma, cytomegalovirus DNA was detected in 114 of 313 specimens (36.4%). Increasing titers of cytomegalovirus DNA were detected in 14 of 26 patients (53.8%).
CONCLUSIONS: The quantitative capture hybrid PCR-ELISA was able to diagnose and monitor cytomegalovirus infection in patients who received bone marrow transplants. Detection of cytomegalovirus DNA in plasma was more predictive of the onset of cytomegalovirus-related clinical symptoms, compared to detection in peripheral blood mononuclear cells.
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- author
- Ziyaeyan, Mazyar ; Sabahi, Farzaneh ; Alborzi, Abdolvahab ; Ramzi, Mani ; Mahboudi, Fereidoun ; Pourabbas, Bahman and Kadivar, Mohammad LU
- publishing date
- 2008-12
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Adolescent, Adult, Biotinylation, Bone Marrow Transplantation, Child, Child, Preschool, Cytomegalovirus, Cytomegalovirus Infections, DNA, Viral, Enzyme-Linked Immunosorbent Assay, Female, Humans, Leukocytes, Mononuclear, Male, Polymerase Chain Reaction, Predictive Value of Tests, Prospective Studies, Time Factors, Transplantation, Homologous, Viral Load, Young Adult, Comparative Study, Evaluation Studies, Journal Article, Research Support, Non-U.S. Gov't
- in
- Experimental and clinical transplantation
- volume
- 6
- issue
- 4
- pages
- 294 - 300
- publisher
- Baskent University
- external identifiers
-
- pmid:19338492
- scopus:65249095975
- ISSN
- 1304-0855
- language
- English
- LU publication?
- no
- id
- abcc8d00-697f-451e-a8d4-5e7b0b0fa058
- date added to LUP
- 2016-10-04 12:59:09
- date last changed
- 2024-01-04 13:45:50
@article{abcc8d00-697f-451e-a8d4-5e7b0b0fa058,
abstract = {{<p>OBJECTIVES: Quantitative monitoring of human cytomegalovirus infections is helpful in determining appropriate antiviral management in patients who receive bone marrow transplants. We sought to design and evaluate a new cytomegalovirus capture hybrid polymerase chain reaction enzyme-linked immunosorbent assay (PCR-ELISA) in plasma and peripheral blood mononuclear cells to monitor cytomegalovirus infection in bone marrow transplant recipients.</p><p>PATIENTS AND METHODS: Twenty-six patients who received allogeneic bone marrow transplants, including 17 male patients and 9 female patients (9 adults, 17 children), were enrolled in this study. A total of 313 consecutive whole blood specimens, before and from 7 to 120 days after transplant, was evaluated in the study. A newly designed biotinylated probe-mediated quantitative competitive PCR-ELISA test was used to determine cytomegalovirus load in specimens of peripheral blood mononuclear cells and plasma.</p><p>RESULTS: All 26 patients were cytomegalovirus seropositive before transplant. Capture hybrid PCR-ELISA of peripheral blood mononuclear cells detected cytomegalovirus DNA in 287 of 313 specimens (91.7%) even in cases with no active cytomegalovirus infection. In plasma, cytomegalovirus DNA was detected in 114 of 313 specimens (36.4%). Increasing titers of cytomegalovirus DNA were detected in 14 of 26 patients (53.8%).</p><p>CONCLUSIONS: The quantitative capture hybrid PCR-ELISA was able to diagnose and monitor cytomegalovirus infection in patients who received bone marrow transplants. Detection of cytomegalovirus DNA in plasma was more predictive of the onset of cytomegalovirus-related clinical symptoms, compared to detection in peripheral blood mononuclear cells.</p>}},
author = {{Ziyaeyan, Mazyar and Sabahi, Farzaneh and Alborzi, Abdolvahab and Ramzi, Mani and Mahboudi, Fereidoun and Pourabbas, Bahman and Kadivar, Mohammad}},
issn = {{1304-0855}},
keywords = {{Adolescent; Adult; Biotinylation; Bone Marrow Transplantation; Child; Child, Preschool; Cytomegalovirus; Cytomegalovirus Infections; DNA, Viral; Enzyme-Linked Immunosorbent Assay; Female; Humans; Leukocytes, Mononuclear; Male; Polymerase Chain Reaction; Predictive Value of Tests; Prospective Studies; Time Factors; Transplantation, Homologous; Viral Load; Young Adult; Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't}},
language = {{eng}},
number = {{4}},
pages = {{294--300}},
publisher = {{Baskent University}},
series = {{Experimental and clinical transplantation}},
title = {{Quantification of human cytomegalovirus DNA by a new capture hybrid polymerase chain reaction enzyme-linked immunosorbent assay in plasma and peripheral blood mononuclear cells of bone marrow transplant recipients}},
volume = {{6}},
year = {{2008}},
}