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Quantification of human cytomegalovirus DNA by a new capture hybrid polymerase chain reaction enzyme-linked immunosorbent assay in plasma and peripheral blood mononuclear cells of bone marrow transplant recipients

Ziyaeyan, Mazyar; Sabahi, Farzaneh; Alborzi, Abdolvahab; Ramzi, Mani; Mahboudi, Fereidoun; Pourabbas, Bahman and Kadivar, Mohammad LU (2008) In Experimental and clinical transplantation : official journal of the Middle East Society for Organ Transplantation 6(4). p.294-300
Abstract

OBJECTIVES: Quantitative monitoring of human cytomegalovirus infections is helpful in determining appropriate antiviral management in patients who receive bone marrow transplants. We sought to design and evaluate a new cytomegalovirus capture hybrid polymerase chain reaction enzyme-linked immunosorbent assay (PCR-ELISA) in plasma and peripheral blood mononuclear cells to monitor cytomegalovirus infection in bone marrow transplant recipients.

PATIENTS AND METHODS: Twenty-six patients who received allogeneic bone marrow transplants, including 17 male patients and 9 female patients (9 adults, 17 children), were enrolled in this study. A total of 313 consecutive whole blood specimens, before and from 7 to 120 days after transplant,... (More)

OBJECTIVES: Quantitative monitoring of human cytomegalovirus infections is helpful in determining appropriate antiviral management in patients who receive bone marrow transplants. We sought to design and evaluate a new cytomegalovirus capture hybrid polymerase chain reaction enzyme-linked immunosorbent assay (PCR-ELISA) in plasma and peripheral blood mononuclear cells to monitor cytomegalovirus infection in bone marrow transplant recipients.

PATIENTS AND METHODS: Twenty-six patients who received allogeneic bone marrow transplants, including 17 male patients and 9 female patients (9 adults, 17 children), were enrolled in this study. A total of 313 consecutive whole blood specimens, before and from 7 to 120 days after transplant, was evaluated in the study. A newly designed biotinylated probe-mediated quantitative competitive PCR-ELISA test was used to determine cytomegalovirus load in specimens of peripheral blood mononuclear cells and plasma.

RESULTS: All 26 patients were cytomegalovirus seropositive before transplant. Capture hybrid PCR-ELISA of peripheral blood mononuclear cells detected cytomegalovirus DNA in 287 of 313 specimens (91.7%) even in cases with no active cytomegalovirus infection. In plasma, cytomegalovirus DNA was detected in 114 of 313 specimens (36.4%). Increasing titers of cytomegalovirus DNA were detected in 14 of 26 patients (53.8%).

CONCLUSIONS: The quantitative capture hybrid PCR-ELISA was able to diagnose and monitor cytomegalovirus infection in patients who received bone marrow transplants. Detection of cytomegalovirus DNA in plasma was more predictive of the onset of cytomegalovirus-related clinical symptoms, compared to detection in peripheral blood mononuclear cells.

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author
publishing date
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Contribution to journal
publication status
published
subject
keywords
Adolescent, Adult, Biotinylation, Bone Marrow Transplantation, Child, Child, Preschool, Cytomegalovirus, Cytomegalovirus Infections, DNA, Viral, Enzyme-Linked Immunosorbent Assay, Female, Humans, Leukocytes, Mononuclear, Male, Polymerase Chain Reaction, Predictive Value of Tests, Prospective Studies, Time Factors, Transplantation, Homologous, Viral Load, Young Adult, Comparative Study, Evaluation Studies, Journal Article, Research Support, Non-U.S. Gov't
in
Experimental and clinical transplantation : official journal of the Middle East Society for Organ Transplantation
volume
6
issue
4
pages
294 - 300
publisher
Baskent University
external identifiers
  • scopus:65249095975
ISSN
1304-0855
language
English
LU publication?
no
id
abcc8d00-697f-451e-a8d4-5e7b0b0fa058
date added to LUP
2016-10-04 12:59:09
date last changed
2017-06-20 13:34:31
@article{abcc8d00-697f-451e-a8d4-5e7b0b0fa058,
  abstract     = {<p>OBJECTIVES: Quantitative monitoring of human cytomegalovirus infections is helpful in determining appropriate antiviral management in patients who receive bone marrow transplants. We sought to design and evaluate a new cytomegalovirus capture hybrid polymerase chain reaction enzyme-linked immunosorbent assay (PCR-ELISA) in plasma and peripheral blood mononuclear cells to monitor cytomegalovirus infection in bone marrow transplant recipients.</p><p>PATIENTS AND METHODS: Twenty-six patients who received allogeneic bone marrow transplants, including 17 male patients and 9 female patients (9 adults, 17 children), were enrolled in this study. A total of 313 consecutive whole blood specimens, before and from 7 to 120 days after transplant, was evaluated in the study. A newly designed biotinylated probe-mediated quantitative competitive PCR-ELISA test was used to determine cytomegalovirus load in specimens of peripheral blood mononuclear cells and plasma.</p><p>RESULTS: All 26 patients were cytomegalovirus seropositive before transplant. Capture hybrid PCR-ELISA of peripheral blood mononuclear cells detected cytomegalovirus DNA in 287 of 313 specimens (91.7%) even in cases with no active cytomegalovirus infection. In plasma, cytomegalovirus DNA was detected in 114 of 313 specimens (36.4%). Increasing titers of cytomegalovirus DNA were detected in 14 of 26 patients (53.8%).</p><p>CONCLUSIONS: The quantitative capture hybrid PCR-ELISA was able to diagnose and monitor cytomegalovirus infection in patients who received bone marrow transplants. Detection of cytomegalovirus DNA in plasma was more predictive of the onset of cytomegalovirus-related clinical symptoms, compared to detection in peripheral blood mononuclear cells.</p>},
  author       = {Ziyaeyan, Mazyar and Sabahi, Farzaneh and Alborzi, Abdolvahab and Ramzi, Mani and Mahboudi, Fereidoun and Pourabbas, Bahman and Kadivar, Mohammad},
  issn         = {1304-0855},
  keyword      = {Adolescent,Adult,Biotinylation,Bone Marrow Transplantation,Child,Child, Preschool,Cytomegalovirus,Cytomegalovirus Infections,DNA, Viral,Enzyme-Linked Immunosorbent Assay,Female,Humans,Leukocytes, Mononuclear,Male,Polymerase Chain Reaction,Predictive Value of Tests,Prospective Studies,Time Factors,Transplantation, Homologous,Viral Load,Young Adult,Comparative Study,Evaluation Studies,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {4},
  pages        = {294--300},
  publisher    = {Baskent University},
  series       = {Experimental and clinical transplantation : official journal of the Middle East Society for Organ Transplantation},
  title        = {Quantification of human cytomegalovirus DNA by a new capture hybrid polymerase chain reaction enzyme-linked immunosorbent assay in plasma and peripheral blood mononuclear cells of bone marrow transplant recipients},
  volume       = {6},
  year         = {2008},
}