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A New Large-scale Pre-purification for Peroxidase from Plant Cell Cultures

Pyo, Sang-Hyun LU ; Hong, Seung-Suh and Kim, Jin-Hyun (2000) In The Korean Society for Biotechnology and Bioengineering 15(4). p.342-345
Abstract
A novel pre-purification method was developed for producing peroxidase to guarantee high purity and yield from plant cell cultures in large-scale process. This method was a simple and efficient procedure for the isolation and pre-purification of peroxidase from the biomass consisting of active clay treatment, followed by cationic exchange chromatography. The use of active clay in the pre-purification process allows for rapid and efficient separation of peroxidase from interfering compounds and dramatically increases yield and purity of crude peroxidase for purification steps compared to alternative processes. This pre-purification process serves to minimize the buffer usage, size and complexity of the HPLC operations for peroxidase... (More)
A novel pre-purification method was developed for producing peroxidase to guarantee high purity and yield from plant cell cultures in large-scale process. This method was a simple and efficient procedure for the isolation and pre-purification of peroxidase from the biomass consisting of active clay treatment, followed by cationic exchange chromatography. The use of active clay in the pre-purification process allows for rapid and efficient separation of peroxidase from interfering compounds and dramatically increases yield and purity of crude peroxidase for purification steps compared to alternative processes. This pre-purification process serves to minimize the buffer usage, size and complexity of the HPLC operations for peroxidase purification. This process is readily scalable to a pilot plant and eventually to a production environment where mass production of material are expected to be produced. (Less)
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type
Contribution to journal
publication status
published
subject
in
The Korean Society for Biotechnology and Bioengineering
volume
15
issue
4
pages
342 - 345
ISSN
1225-7117
language
Korean
LU publication?
no
id
abd9e39e-d594-4efa-964b-a9f332ac4217
alternative location
https://koreascience.kr/article/JAKO200011919976523.pub
date added to LUP
2025-09-19 11:46:04
date last changed
2025-09-25 10:24:55
@article{abd9e39e-d594-4efa-964b-a9f332ac4217,
  abstract     = {{A novel pre-purification method was developed for producing peroxidase to guarantee high purity and yield from plant cell cultures in large-scale process. This method was a simple and efficient procedure for the isolation and pre-purification of peroxidase from the biomass consisting of active clay treatment, followed by cationic exchange chromatography. The use of active clay in the pre-purification process allows for rapid and efficient separation of peroxidase from interfering compounds and dramatically increases yield and purity of crude peroxidase for purification steps compared to alternative processes. This pre-purification process serves to minimize the buffer usage, size and complexity of the HPLC operations for peroxidase purification. This process is readily scalable to a pilot plant and eventually to a production environment where mass production of material are expected to be produced.}},
  author       = {{Pyo, Sang-Hyun and Hong, Seung-Suh and Kim, Jin-Hyun}},
  issn         = {{1225-7117}},
  language     = {{kor}},
  number       = {{4}},
  pages        = {{342--345}},
  series       = {{The Korean Society for Biotechnology and Bioengineering}},
  title        = {{A New Large-scale Pre-purification for Peroxidase from Plant Cell Cultures}},
  url          = {{https://koreascience.kr/article/JAKO200011919976523.pub}},
  volume       = {{15}},
  year         = {{2000}},
}