The matrisome landscape controlling in vivo germ cell fates
(2024) In Nature Communications 15. p.1-16- Abstract
The developmental fate of cells is regulated by intrinsic factors and the extracellular environment. The extracellular matrix (matrisome) delivers chemical and mechanical cues that can modify cellular development. However, comprehensive understanding of how matrisome factors control cells in vivo is lacking. Here we show that specific matrisome factors act individually and collectively to control germ cell development. Surveying development of undifferentiated germline stem cells through to mature oocytes in the Caenorhabditis elegans germ line enabled holistic functional analysis of 443 conserved matrisome-coding genes. Using high-content imaging, 3D reconstruction, and cell behavior analysis, we identify 321 matrisome genes that... (More)
The developmental fate of cells is regulated by intrinsic factors and the extracellular environment. The extracellular matrix (matrisome) delivers chemical and mechanical cues that can modify cellular development. However, comprehensive understanding of how matrisome factors control cells in vivo is lacking. Here we show that specific matrisome factors act individually and collectively to control germ cell development. Surveying development of undifferentiated germline stem cells through to mature oocytes in the Caenorhabditis elegans germ line enabled holistic functional analysis of 443 conserved matrisome-coding genes. Using high-content imaging, 3D reconstruction, and cell behavior analysis, we identify 321 matrisome genes that impact germ cell development, the majority of which (>80%) are undescribed. Our analysis identifies key matrisome networks acting autonomously and non-autonomously to coordinate germ cell behavior. Further, our results demonstrate that germ cell development requires continual remodeling of the matrisome landscape. Together, this study provides a comprehensive platform for deciphering how extracellular signaling controls cellular development and anticipate this will establish new opportunities for manipulating cell fates.
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- author
- Amran, Aqilah LU ; Pigatto, Lara LU ; Farley, Johanna LU ; Godini, Rasoul ; Pocock, Roger and Gopal, Sandeep LU
- organization
- publishing date
- 2024-05-17
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Animals, Extracellular Matrix/metabolism, Germ Cells/metabolism, Cell Differentiation/genetics, Proteins/metabolism, Gene Expression Regulation, Developmental, Signal Transduction, Cell Lineage/genetics, Oocytes/metabolism
- in
- Nature Communications
- volume
- 15
- article number
- 4200
- pages
- 1 - 16
- publisher
- Nature Publishing Group
- external identifiers
-
- scopus:85193488663
- pmid:38760342
- ISSN
- 2041-1723
- DOI
- 10.1038/s41467-024-48283-4
- language
- English
- LU publication?
- yes
- additional info
- © 2024. The Author(s).
- id
- ac10dc31-8a16-458b-922c-b7d6da6011ed
- date added to LUP
- 2024-05-19 09:12:05
- date last changed
- 2024-06-14 11:45:33
@article{ac10dc31-8a16-458b-922c-b7d6da6011ed, abstract = {{<p>The developmental fate of cells is regulated by intrinsic factors and the extracellular environment. The extracellular matrix (matrisome) delivers chemical and mechanical cues that can modify cellular development. However, comprehensive understanding of how matrisome factors control cells in vivo is lacking. Here we show that specific matrisome factors act individually and collectively to control germ cell development. Surveying development of undifferentiated germline stem cells through to mature oocytes in the Caenorhabditis elegans germ line enabled holistic functional analysis of 443 conserved matrisome-coding genes. Using high-content imaging, 3D reconstruction, and cell behavior analysis, we identify 321 matrisome genes that impact germ cell development, the majority of which (>80%) are undescribed. Our analysis identifies key matrisome networks acting autonomously and non-autonomously to coordinate germ cell behavior. Further, our results demonstrate that germ cell development requires continual remodeling of the matrisome landscape. Together, this study provides a comprehensive platform for deciphering how extracellular signaling controls cellular development and anticipate this will establish new opportunities for manipulating cell fates.</p>}}, author = {{Amran, Aqilah and Pigatto, Lara and Farley, Johanna and Godini, Rasoul and Pocock, Roger and Gopal, Sandeep}}, issn = {{2041-1723}}, keywords = {{Animals; Extracellular Matrix/metabolism; Germ Cells/metabolism; Cell Differentiation/genetics; Proteins/metabolism; Gene Expression Regulation, Developmental; Signal Transduction; Cell Lineage/genetics; Oocytes/metabolism}}, language = {{eng}}, month = {{05}}, pages = {{1--16}}, publisher = {{Nature Publishing Group}}, series = {{Nature Communications}}, title = {{The matrisome landscape controlling in vivo germ cell fates}}, url = {{http://dx.doi.org/10.1038/s41467-024-48283-4}}, doi = {{10.1038/s41467-024-48283-4}}, volume = {{15}}, year = {{2024}}, }