Linking in Vitro Effects and Detected Organic Micropollutants in Surface Water Using Mixture-Toxicity Modeling
(2015) In Environmental Science & Technology 49(24). p.14614-14624- Abstract
- Surface water can contain countless organic micropollutants, and targeted chemical analysis alone may only detect a small fraction of the chemicals present. Consequently, bioanalytical tools can be applied complementary to chemical analysis to detect the effects of complex chemical mixtures. In this study, bioassays indicative of activation of the aryl hydrocarbon receptor (AhR), activation of the pregnane X receptor (PXR), activation of the estrogen receptor (ER), adaptive stress responses to oxidative stress (Nrf2), genotoxicity (p53) and inflammation (NF-κB) and the fish embryo toxicity test were applied along with chemical analysis to water extracts from the Danube River. Mixture-toxicity modeling was applied to determine the... (More)
- Surface water can contain countless organic micropollutants, and targeted chemical analysis alone may only detect a small fraction of the chemicals present. Consequently, bioanalytical tools can be applied complementary to chemical analysis to detect the effects of complex chemical mixtures. In this study, bioassays indicative of activation of the aryl hydrocarbon receptor (AhR), activation of the pregnane X receptor (PXR), activation of the estrogen receptor (ER), adaptive stress responses to oxidative stress (Nrf2), genotoxicity (p53) and inflammation (NF-κB) and the fish embryo toxicity test were applied along with chemical analysis to water extracts from the Danube River. Mixture-toxicity modeling was applied to determine the contribution of detected chemicals to the biological effect. Effect concentrations for between 0 to 13 detected chemicals could be found in the literature for the different bioassays. Detected chemicals explained less than 0.2% of the biological effect in the PXR activation, adaptive stress response, and fish embryo toxicity assays, while five chemicals explained up to 80% of ER activation, and three chemicals explained up to 71% of AhR activation. This study highlights the importance of fingerprinting the effects of detected chemicals. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/ac1ec8e8-9e70-43ec-97e5-4fa6855983eb
- author
- publishing date
- 2015-10-30
- type
- Contribution to journal
- publication status
- published
- in
- Environmental Science & Technology
- volume
- 49
- issue
- 24
- pages
- 14614 - 14624
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- scopus:84950137463
- ISSN
- 0013-936X
- DOI
- 10.1021/acs.est.5b04083
- language
- English
- LU publication?
- no
- id
- ac1ec8e8-9e70-43ec-97e5-4fa6855983eb
- date added to LUP
- 2019-04-18 14:51:01
- date last changed
- 2022-04-02 08:07:25
@article{ac1ec8e8-9e70-43ec-97e5-4fa6855983eb, abstract = {{Surface water can contain countless organic micropollutants, and targeted chemical analysis alone may only detect a small fraction of the chemicals present. Consequently, bioanalytical tools can be applied complementary to chemical analysis to detect the effects of complex chemical mixtures. In this study, bioassays indicative of activation of the aryl hydrocarbon receptor (AhR), activation of the pregnane X receptor (PXR), activation of the estrogen receptor (ER), adaptive stress responses to oxidative stress (Nrf2), genotoxicity (p53) and inflammation (NF-κB) and the fish embryo toxicity test were applied along with chemical analysis to water extracts from the Danube River. Mixture-toxicity modeling was applied to determine the contribution of detected chemicals to the biological effect. Effect concentrations for between 0 to 13 detected chemicals could be found in the literature for the different bioassays. Detected chemicals explained less than 0.2% of the biological effect in the PXR activation, adaptive stress response, and fish embryo toxicity assays, while five chemicals explained up to 80% of ER activation, and three chemicals explained up to 71% of AhR activation. This study highlights the importance of fingerprinting the effects of detected chemicals.}}, author = {{Neale, Peta A. and Ait-aissa, Selim and Brack, Werner and Creusot, Nicolas and Denison, Michael S. and Deutschmann, Björn and Hilscherová, Klára and Hollert, Henner and Krauss, Martin and Novák, Jiří and Schulze, Tobias and Seiler, Thomas-benjamin and Serra, Helene and Shao, Ying and Escher, Beate I.}}, issn = {{0013-936X}}, language = {{eng}}, month = {{10}}, number = {{24}}, pages = {{14614--14624}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Environmental Science & Technology}}, title = {{Linking in Vitro Effects and Detected Organic Micropollutants in Surface Water Using Mixture-Toxicity Modeling}}, url = {{http://dx.doi.org/10.1021/acs.est.5b04083}}, doi = {{10.1021/acs.est.5b04083}}, volume = {{49}}, year = {{2015}}, }