Vesicles Balance Osmotic Stress with Bending Energy That Can Be Released to Form Daughter Vesicles
(2022) In Journal of Physical Chemistry Letters 13(2). p.498-507- Abstract
The bending energy of the lipid membrane is central to biological processes involving vesicles, such as endocytosis and exocytosis. To illustrate the role of bending energy in these processes, we study the response of single-component giant unilamellar vesicles (GUVs) subjected to external osmotic stress by glucose addition. For osmotic pressures exceeding 0.15 atm, an abrupt shape change from spherical to prolate occurs, showing that the osmotic pressure is balanced by the free energy of membrane bending. After equilibration, the external glucose solution was exchanged for pure water, yielding rapid formation of monodisperse daughter vesicles inside the GUVs through an endocytosis-like process. Our theoretical analysis shows that this... (More)
The bending energy of the lipid membrane is central to biological processes involving vesicles, such as endocytosis and exocytosis. To illustrate the role of bending energy in these processes, we study the response of single-component giant unilamellar vesicles (GUVs) subjected to external osmotic stress by glucose addition. For osmotic pressures exceeding 0.15 atm, an abrupt shape change from spherical to prolate occurs, showing that the osmotic pressure is balanced by the free energy of membrane bending. After equilibration, the external glucose solution was exchanged for pure water, yielding rapid formation of monodisperse daughter vesicles inside the GUVs through an endocytosis-like process. Our theoretical analysis shows that this process requires significant free energies stored in the deformed membrane to be kinetically allowed. The results indicate that bending energies stored in GUVs are much higher than previously implicated, with potential consequences for vesicle fusion/fission and the osmotic regulation in living cells.
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- author
- Liu, Xiaoyan LU ; Stenhammar, Joakim LU ; Wennerström, Håkan LU and Sparr, Emma LU
- organization
- publishing date
- 2022-01-20
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Physical Chemistry Letters
- volume
- 13
- issue
- 2
- pages
- 10 pages
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- scopus:85123812970
- pmid:35005979
- ISSN
- 1948-7185
- DOI
- 10.1021/acs.jpclett.1c03369
- language
- English
- LU publication?
- yes
- additional info
- Publisher Copyright: © 2022 The Authors. Published by American Chemical Society.
- id
- ac40e67a-b37b-49cc-accc-edad73210b22
- date added to LUP
- 2022-02-16 13:07:03
- date last changed
- 2024-04-18 05:40:00
@article{ac40e67a-b37b-49cc-accc-edad73210b22,
abstract = {{<p>The bending energy of the lipid membrane is central to biological processes involving vesicles, such as endocytosis and exocytosis. To illustrate the role of bending energy in these processes, we study the response of single-component giant unilamellar vesicles (GUVs) subjected to external osmotic stress by glucose addition. For osmotic pressures exceeding 0.15 atm, an abrupt shape change from spherical to prolate occurs, showing that the osmotic pressure is balanced by the free energy of membrane bending. After equilibration, the external glucose solution was exchanged for pure water, yielding rapid formation of monodisperse daughter vesicles inside the GUVs through an endocytosis-like process. Our theoretical analysis shows that this process requires significant free energies stored in the deformed membrane to be kinetically allowed. The results indicate that bending energies stored in GUVs are much higher than previously implicated, with potential consequences for vesicle fusion/fission and the osmotic regulation in living cells.</p>}},
author = {{Liu, Xiaoyan and Stenhammar, Joakim and Wennerström, Håkan and Sparr, Emma}},
issn = {{1948-7185}},
language = {{eng}},
month = {{01}},
number = {{2}},
pages = {{498--507}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Journal of Physical Chemistry Letters}},
title = {{Vesicles Balance Osmotic Stress with Bending Energy That Can Be Released to Form Daughter Vesicles}},
url = {{http://dx.doi.org/10.1021/acs.jpclett.1c03369}},
doi = {{10.1021/acs.jpclett.1c03369}},
volume = {{13}},
year = {{2022}},
}