Block in insulin release from column-perifused pancreatic β-cells induced by islet cell surface antibodies and complement
Kanatsuna, T. ; Lernmark, A. LU
; Rubenstein, A. H.
and Steiner, D. F.
(1981)
In Diabetes
30(3).
p.231-234
- Abstract
Dispersed rat pancreatic islet cells were mixed into a short column of Bio-Gel P-2 polyacrylamide beads and perifused with an antiserum containing islet cell surface antibodies. The release of radioactive chromium from prelabeled cells, as a measure of cell membrane permeability, was not affected by cell surface antibodies alone, but increased dramatically in the presence of complement. While there was an eightfold increase in glucose-stimulated insulin release from β-cells exposed to control serum and complement, insulin release was completely blocked from β-cells exposed to islet-cell-specific antibodies and complement. These findings suggest that islet cell surface antibodies can mediate complement-dependent cytotoxicity.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/ac6a203d-0cad-4975-afd3-7992b51aeac2
- author
- Kanatsuna, T.
; Lernmark, A.
LU
; Rubenstein, A. H.
and Steiner, D. F.
- publishing date
- 1981-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Diabetes
- volume
- 30
- issue
- 3
- pages
- 4 pages
- publisher
- American Diabetes Association Inc.
- external identifiers
-
- scopus:0019411515
- pmid:7009272
- ISSN
- 0012-1797
- DOI
- 10.2337/diab.30.3.231
- language
- English
- LU publication?
- no
- id
- ac6a203d-0cad-4975-afd3-7992b51aeac2
- date added to LUP
- 2019-09-16 15:36:00
- date last changed
- 2024-03-13 08:01:04
@article{ac6a203d-0cad-4975-afd3-7992b51aeac2,
abstract = {{<p>Dispersed rat pancreatic islet cells were mixed into a short column of Bio-Gel P-2 polyacrylamide beads and perifused with an antiserum containing islet cell surface antibodies. The release of radioactive chromium from prelabeled cells, as a measure of cell membrane permeability, was not affected by cell surface antibodies alone, but increased dramatically in the presence of complement. While there was an eightfold increase in glucose-stimulated insulin release from β-cells exposed to control serum and complement, insulin release was completely blocked from β-cells exposed to islet-cell-specific antibodies and complement. These findings suggest that islet cell surface antibodies can mediate complement-dependent cytotoxicity.</p>}},
author = {{Kanatsuna, T. and Lernmark, A. and Rubenstein, A. H. and Steiner, D. F.}},
issn = {{0012-1797}},
language = {{eng}},
month = {{01}},
number = {{3}},
pages = {{231--234}},
publisher = {{American Diabetes Association Inc.}},
series = {{Diabetes}},
title = {{Block in insulin release from column-perifused pancreatic β-cells induced by islet cell surface antibodies and complement}},
url = {{http://dx.doi.org/10.2337/diab.30.3.231}},
doi = {{10.2337/diab.30.3.231}},
volume = {{30}},
year = {{1981}},
}