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Thermoregulation via Temperature-Dependent PGD2 Production in Mouse Preoptic Area

Wang, Tongfei A; Teo, Chin Fen; Åkerblom, Malin LU ; Chen, Chao; Tynan-La Fontaine, Marena; Greiner, Vanille Juliette; Diaz, Aaron; McManus, Michael T; Jan, Yuh Nung and Jan, Lily Y (2019) In Neuron 103(2). p.7-322
Abstract

Body temperature control is essential for survival. In mammals, thermoregulation is mediated by the preoptic area of anterior hypothalamus (POA), with ∼30% of its neurons sensitive to brain temperature change. It is still unknown whether and how these temperature-sensitive neurons are involved in thermoregulation, because for eight decades they have only been identified via electrophysiological recording. By combining single-cell RNA-seq with whole-cell patch-clamp recordings, we identified Ptgds as a genetic marker for temperature-sensitive POA neurons. Then, we demonstrated these neurons' role in thermoregulation via chemogenetics. Given that Ptgds encodes the enzyme that synthesizes prostaglandin D2 (PGD2), we further explored its... (More)

Body temperature control is essential for survival. In mammals, thermoregulation is mediated by the preoptic area of anterior hypothalamus (POA), with ∼30% of its neurons sensitive to brain temperature change. It is still unknown whether and how these temperature-sensitive neurons are involved in thermoregulation, because for eight decades they have only been identified via electrophysiological recording. By combining single-cell RNA-seq with whole-cell patch-clamp recordings, we identified Ptgds as a genetic marker for temperature-sensitive POA neurons. Then, we demonstrated these neurons' role in thermoregulation via chemogenetics. Given that Ptgds encodes the enzyme that synthesizes prostaglandin D2 (PGD2), we further explored its role in thermoregulation. Our study revealed that rising temperature of POA alters the activity of Ptgds-expressing neurons so as to increase PGD2 production. PGD2 activates its receptor DP1 and excites downstream neurons in the ventral medial preoptic area (vMPO) that mediates body temperature decrease, a negative feedback loop for thermoregulation.

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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Neuron
volume
103
issue
2
pages
7 - 322
publisher
Cell Press
external identifiers
  • scopus:85068139923
ISSN
0896-6273
DOI
10.1016/j.neuron.2019.04.035
language
English
LU publication?
no
id
ac7bfb88-687e-49e2-b208-f00d2aae6d7c
date added to LUP
2019-08-13 13:39:52
date last changed
2019-08-18 05:02:07
@article{ac7bfb88-687e-49e2-b208-f00d2aae6d7c,
  abstract     = {<p>Body temperature control is essential for survival. In mammals, thermoregulation is mediated by the preoptic area of anterior hypothalamus (POA), with ∼30% of its neurons sensitive to brain temperature change. It is still unknown whether and how these temperature-sensitive neurons are involved in thermoregulation, because for eight decades they have only been identified via electrophysiological recording. By combining single-cell RNA-seq with whole-cell patch-clamp recordings, we identified Ptgds as a genetic marker for temperature-sensitive POA neurons. Then, we demonstrated these neurons' role in thermoregulation via chemogenetics. Given that Ptgds encodes the enzyme that synthesizes prostaglandin D2 (PGD2), we further explored its role in thermoregulation. Our study revealed that rising temperature of POA alters the activity of Ptgds-expressing neurons so as to increase PGD2 production. PGD2 activates its receptor DP1 and excites downstream neurons in the ventral medial preoptic area (vMPO) that mediates body temperature decrease, a negative feedback loop for thermoregulation.</p>},
  author       = {Wang, Tongfei A and Teo, Chin Fen and Åkerblom, Malin and Chen, Chao and Tynan-La Fontaine, Marena and Greiner, Vanille Juliette and Diaz, Aaron and McManus, Michael T and Jan, Yuh Nung and Jan, Lily Y},
  issn         = {0896-6273},
  language     = {eng},
  month        = {07},
  number       = {2},
  pages        = {7--322},
  publisher    = {Cell Press},
  series       = {Neuron},
  title        = {Thermoregulation via Temperature-Dependent PGD2 Production in Mouse Preoptic Area},
  url          = {http://dx.doi.org/10.1016/j.neuron.2019.04.035},
  volume       = {103},
  year         = {2019},
}