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Isolation and characterization of a tumor necrosis factor binding protein from urine

Olsson, Inge LU ; Lantz, Mikael ; Nilsson, Eva ; Peetre, Christina ; Thysell, Hans LU ; Grubb, Anders LU orcid and Adolf, G (1989) In European Journal of Haematology 42(3). p.270-275
Abstract

Tumor necrosis factor (TNF)/cachectin can produce both beneficial and harmful manifestations. Mechanisms may operate to counteract potentially harmful effects such as shock and cachexia. The TNF binding protein (TNF-BP), which is found at increased levels in serum and urine of patients with chronic renal failure, may play such a role. TNF-BP was purified 1,000,000-fold to homogeneity from urine of patients with chronic renal failure by use of ion exchange chromatography, affinity chromatography on TNF-Sepharose and reverse phase chromatography. The purified protein contained only one chain with an apparent Mr on sodium dodecyl sulfate-polyacrylamide gel electrophoresis of 30,000. The aminoterminal amino acid sequence... (More)

Tumor necrosis factor (TNF)/cachectin can produce both beneficial and harmful manifestations. Mechanisms may operate to counteract potentially harmful effects such as shock and cachexia. The TNF binding protein (TNF-BP), which is found at increased levels in serum and urine of patients with chronic renal failure, may play such a role. TNF-BP was purified 1,000,000-fold to homogeneity from urine of patients with chronic renal failure by use of ion exchange chromatography, affinity chromatography on TNF-Sepharose and reverse phase chromatography. The purified protein contained only one chain with an apparent Mr on sodium dodecyl sulfate-polyacrylamide gel electrophoresis of 30,000. The aminoterminal amino acid sequence D-S-V-X-P-Q-G-K-Y-I-H-P-Q-V-N-S-I-X-K-T revealed no significant homologies with previously described protein sequences. TNF-BP may act as a regulator of the bioactivities of TNF/cachectin.

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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Amino Acid Sequence, Carrier Proteins/isolation & purification, Chromatography, Affinity, Chromatography, Ion Exchange, Electrophoresis, Polyacrylamide Gel, Humans, Kidney Failure, Chronic/urine, Molecular Sequence Data, Tumor Necrosis Factor-alpha/metabolism
in
European Journal of Haematology
volume
42
issue
3
pages
270 - 275
publisher
Wiley-Blackwell
external identifiers
  • scopus:0024600484
  • pmid:2924890
ISSN
0902-4441
DOI
10.1111/j.1600-0609.1989.tb00111.x
language
English
LU publication?
yes
id
ac864e9b-e5c1-4fe4-be2f-7c4e9cad8a56
date added to LUP
2021-10-27 09:22:41
date last changed
2024-01-12 02:57:36
@article{ac864e9b-e5c1-4fe4-be2f-7c4e9cad8a56,
  abstract     = {{<p>Tumor necrosis factor (TNF)/cachectin can produce both beneficial and harmful manifestations. Mechanisms may operate to counteract potentially harmful effects such as shock and cachexia. The TNF binding protein (TNF-BP), which is found at increased levels in serum and urine of patients with chronic renal failure, may play such a role. TNF-BP was purified 1,000,000-fold to homogeneity from urine of patients with chronic renal failure by use of ion exchange chromatography, affinity chromatography on TNF-Sepharose and reverse phase chromatography. The purified protein contained only one chain with an apparent Mr on sodium dodecyl sulfate-polyacrylamide gel electrophoresis of 30,000. The aminoterminal amino acid sequence D-S-V-X-P-Q-G-K-Y-I-H-P-Q-V-N-S-I-X-K-T revealed no significant homologies with previously described protein sequences. TNF-BP may act as a regulator of the bioactivities of TNF/cachectin.</p>}},
  author       = {{Olsson, Inge and Lantz, Mikael and Nilsson, Eva and Peetre, Christina and Thysell, Hans and Grubb, Anders and Adolf, G}},
  issn         = {{0902-4441}},
  keywords     = {{Amino Acid Sequence; Carrier Proteins/isolation & purification; Chromatography, Affinity; Chromatography, Ion Exchange; Electrophoresis, Polyacrylamide Gel; Humans; Kidney Failure, Chronic/urine; Molecular Sequence Data; Tumor Necrosis Factor-alpha/metabolism}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{270--275}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{European Journal of Haematology}},
  title        = {{Isolation and characterization of a tumor necrosis factor binding protein from urine}},
  url          = {{http://dx.doi.org/10.1111/j.1600-0609.1989.tb00111.x}},
  doi          = {{10.1111/j.1600-0609.1989.tb00111.x}},
  volume       = {{42}},
  year         = {{1989}},
}