Isolation and characterization of a tumor necrosis factor binding protein from urine
(1989) In European Journal of Haematology 42(3). p.270-275- Abstract
Tumor necrosis factor (TNF)/cachectin can produce both beneficial and harmful manifestations. Mechanisms may operate to counteract potentially harmful effects such as shock and cachexia. The TNF binding protein (TNF-BP), which is found at increased levels in serum and urine of patients with chronic renal failure, may play such a role. TNF-BP was purified 1,000,000-fold to homogeneity from urine of patients with chronic renal failure by use of ion exchange chromatography, affinity chromatography on TNF-Sepharose and reverse phase chromatography. The purified protein contained only one chain with an apparent Mr on sodium dodecyl sulfate-polyacrylamide gel electrophoresis of 30,000. The aminoterminal amino acid sequence... (More)
Tumor necrosis factor (TNF)/cachectin can produce both beneficial and harmful manifestations. Mechanisms may operate to counteract potentially harmful effects such as shock and cachexia. The TNF binding protein (TNF-BP), which is found at increased levels in serum and urine of patients with chronic renal failure, may play such a role. TNF-BP was purified 1,000,000-fold to homogeneity from urine of patients with chronic renal failure by use of ion exchange chromatography, affinity chromatography on TNF-Sepharose and reverse phase chromatography. The purified protein contained only one chain with an apparent Mr on sodium dodecyl sulfate-polyacrylamide gel electrophoresis of 30,000. The aminoterminal amino acid sequence D-S-V-X-P-Q-G-K-Y-I-H-P-Q-V-N-S-I-X-K-T revealed no significant homologies with previously described protein sequences. TNF-BP may act as a regulator of the bioactivities of TNF/cachectin.
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- author
- Olsson, Inge LU ; Lantz, Mikael ; Nilsson, Eva ; Peetre, Christina ; Thysell, Hans LU ; Grubb, Anders LU and Adolf, G
- organization
- publishing date
- 1989
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Amino Acid Sequence, Carrier Proteins/isolation & purification, Chromatography, Affinity, Chromatography, Ion Exchange, Electrophoresis, Polyacrylamide Gel, Humans, Kidney Failure, Chronic/urine, Molecular Sequence Data, Tumor Necrosis Factor-alpha/metabolism
- in
- European Journal of Haematology
- volume
- 42
- issue
- 3
- pages
- 270 - 275
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:0024600484
- pmid:2924890
- ISSN
- 0902-4441
- DOI
- 10.1111/j.1600-0609.1989.tb00111.x
- language
- English
- LU publication?
- yes
- id
- ac864e9b-e5c1-4fe4-be2f-7c4e9cad8a56
- date added to LUP
- 2021-10-27 09:22:41
- date last changed
- 2024-01-12 02:57:36
@article{ac864e9b-e5c1-4fe4-be2f-7c4e9cad8a56, abstract = {{<p>Tumor necrosis factor (TNF)/cachectin can produce both beneficial and harmful manifestations. Mechanisms may operate to counteract potentially harmful effects such as shock and cachexia. The TNF binding protein (TNF-BP), which is found at increased levels in serum and urine of patients with chronic renal failure, may play such a role. TNF-BP was purified 1,000,000-fold to homogeneity from urine of patients with chronic renal failure by use of ion exchange chromatography, affinity chromatography on TNF-Sepharose and reverse phase chromatography. The purified protein contained only one chain with an apparent Mr on sodium dodecyl sulfate-polyacrylamide gel electrophoresis of 30,000. The aminoterminal amino acid sequence D-S-V-X-P-Q-G-K-Y-I-H-P-Q-V-N-S-I-X-K-T revealed no significant homologies with previously described protein sequences. TNF-BP may act as a regulator of the bioactivities of TNF/cachectin.</p>}}, author = {{Olsson, Inge and Lantz, Mikael and Nilsson, Eva and Peetre, Christina and Thysell, Hans and Grubb, Anders and Adolf, G}}, issn = {{0902-4441}}, keywords = {{Amino Acid Sequence; Carrier Proteins/isolation & purification; Chromatography, Affinity; Chromatography, Ion Exchange; Electrophoresis, Polyacrylamide Gel; Humans; Kidney Failure, Chronic/urine; Molecular Sequence Data; Tumor Necrosis Factor-alpha/metabolism}}, language = {{eng}}, number = {{3}}, pages = {{270--275}}, publisher = {{Wiley-Blackwell}}, series = {{European Journal of Haematology}}, title = {{Isolation and characterization of a tumor necrosis factor binding protein from urine}}, url = {{http://dx.doi.org/10.1111/j.1600-0609.1989.tb00111.x}}, doi = {{10.1111/j.1600-0609.1989.tb00111.x}}, volume = {{42}}, year = {{1989}}, }