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In situ detection of cancerous kidney tissue by means of fiber ATR-FTIR spectroscopy

Sablinskas, Valdas; Velicka, Martynas; Pucetaite, Milda LU ; Urboniene, Vidita; Ceponkus, Justinas; Bandzeviciute, Rimante; Jankevicius, Feliksas; Sakharova, Tatiana; Bibikova, Olga and Steiner, Gerald (2018) Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI 2018 In Proceedings of SPIE 10497.
Abstract

The crucial goal of kidney-sparing surgical resection of a malignant tumor is complete removal of the cancerous tissue. The exact border between the cancerous and normal tissues is not always possible to identify by naked eye, therefore, a supplementary intraoperative diagnosis is needed. Unfortunately, intraoperative pathology methods used nowadays are time consuming and of inadequate quality rendering not definitive diagnosis. It has recently been shown that ATR-FTIR spectroscopy can be used for fast discrimination between cancerous and normal kidney tissues by analyzing the collected spectra of the tissue touch imprint smears. Most prominent differences are obtained in the wavenumber region from 950 cm-1 to 1250... (More)

The crucial goal of kidney-sparing surgical resection of a malignant tumor is complete removal of the cancerous tissue. The exact border between the cancerous and normal tissues is not always possible to identify by naked eye, therefore, a supplementary intraoperative diagnosis is needed. Unfortunately, intraoperative pathology methods used nowadays are time consuming and of inadequate quality rendering not definitive diagnosis. It has recently been shown that ATR-FTIR spectroscopy can be used for fast discrimination between cancerous and normal kidney tissues by analyzing the collected spectra of the tissue touch imprint smears. Most prominent differences are obtained in the wavenumber region from 950 cm-1 to 1250 cm-1, where the spectral bands due to the molecular vibrations of glycogen arise in the spectra of cancerous tissue smears. Such method of detection of cancerous tissue is limited by requirement to transfer the suspected tissue from the body to the FTIR instrument and stamp it on an ATR crystal of the spectrometer. We propose a spectroscopic tool which exploits the same principle of detection of cancerous cells as mentioned above, but does not require the tissue to be transferred from the body to the spectrometer. The portable spectrometer used in this design is equipped with fiber ATR probe and a sensitive liquid nitrogen cooled MCT detector. The design of the fiber probe allows the ATR tip to be changed easily in order to use only new sterilized tips for each measurement point of the tissue. It also enables sampling multiple areas of the suspected tissue with high lateral resolution which, in turn, increases accuracy with which the marginal regions between normal and cancerous tissues can be identified. Due to the loss of optical signal in the fiber probe the spectra have lower signal-To-noise ratio than in the case of standard ATR sampling setup. However, software for the spectral analysis used with the fiber probe design is still able to distinguish between cancerous and normal tissues with high accuracy.

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Please use this url to cite or link to this publication:
author
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
keywords
ATR, Fiber probe, FTIR, Kidney cancer, Tissue impression smears
host publication
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI
series title
Proceedings of SPIE
editor
Nicolau, Dan V.; Farkas, Daniel L.; Leif, Robert C.; ; and
volume
10497
publisher
SPIE
conference name
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI 2018
conference location
San Francisco, United States
conference dates
2018-01-29 - 2018-01-31
external identifiers
  • scopus:85046335661
ISSN
0277-786X
1996-756X
ISBN
9781510614796
DOI
10.1117/12.2289393
language
English
LU publication?
no
id
acc4d98b-76a2-46a9-9925-d5987d71d5b2
date added to LUP
2019-03-18 11:52:45
date last changed
2019-03-21 15:43:42
@inproceedings{acc4d98b-76a2-46a9-9925-d5987d71d5b2,
  abstract     = {<p>The crucial goal of kidney-sparing surgical resection of a malignant tumor is complete removal of the cancerous tissue. The exact border between the cancerous and normal tissues is not always possible to identify by naked eye, therefore, a supplementary intraoperative diagnosis is needed. Unfortunately, intraoperative pathology methods used nowadays are time consuming and of inadequate quality rendering not definitive diagnosis. It has recently been shown that ATR-FTIR spectroscopy can be used for fast discrimination between cancerous and normal kidney tissues by analyzing the collected spectra of the tissue touch imprint smears. Most prominent differences are obtained in the wavenumber region from 950 cm<sup>-1</sup> to 1250 cm<sup>-1</sup>, where the spectral bands due to the molecular vibrations of glycogen arise in the spectra of cancerous tissue smears. Such method of detection of cancerous tissue is limited by requirement to transfer the suspected tissue from the body to the FTIR instrument and stamp it on an ATR crystal of the spectrometer. We propose a spectroscopic tool which exploits the same principle of detection of cancerous cells as mentioned above, but does not require the tissue to be transferred from the body to the spectrometer. The portable spectrometer used in this design is equipped with fiber ATR probe and a sensitive liquid nitrogen cooled MCT detector. The design of the fiber probe allows the ATR tip to be changed easily in order to use only new sterilized tips for each measurement point of the tissue. It also enables sampling multiple areas of the suspected tissue with high lateral resolution which, in turn, increases accuracy with which the marginal regions between normal and cancerous tissues can be identified. Due to the loss of optical signal in the fiber probe the spectra have lower signal-To-noise ratio than in the case of standard ATR sampling setup. However, software for the spectral analysis used with the fiber probe design is still able to distinguish between cancerous and normal tissues with high accuracy.</p>},
  author       = {Sablinskas, Valdas and Velicka, Martynas and Pucetaite, Milda and Urboniene, Vidita and Ceponkus, Justinas and Bandzeviciute, Rimante and Jankevicius, Feliksas and Sakharova, Tatiana and Bibikova, Olga and Steiner, Gerald},
  booktitle    = {Proceedings of SPIE},
  editor       = {Nicolau, Dan V. and Farkas, Daniel L. and Leif, Robert C.},
  isbn         = {9781510614796},
  issn         = {0277-786X},
  keyword      = {ATR,Fiber probe,FTIR,Kidney cancer,Tissue impression smears},
  language     = {eng},
  location     = {San Francisco, United States},
  month        = {01},
  publisher    = {SPIE},
  title        = {In situ detection of cancerous kidney tissue by means of fiber ATR-FTIR spectroscopy},
  url          = {http://dx.doi.org/10.1117/12.2289393},
  volume       = {10497},
  year         = {2018},
}