Capture of neuroepithelial-like stem cells from pluripotent stem cells provides a versatile system for in vitro production of human neurons
(2012) In PLoS ONE 7(1).- Abstract
Human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC) provide new prospects for studying human neurodevelopment and modeling neurological disease. In particular, iPSC-derived neural cells permit a direct comparison of disease-relevant molecular pathways in neurons and glia derived from patients and healthy individuals. A prerequisite for such comparative studies are robust protocols that efficiently yield standardized populations of neural cell types. Here we show that long-term self-renewing neuroepithelial-like stem cells (lt-NES cells) derived from 3 hESC and 6 iPSC lines in two independent laboratories exhibit consistent characteristics including i) continuous expandability in the presence of FGF2 and EGF; ii)... (More)
Human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC) provide new prospects for studying human neurodevelopment and modeling neurological disease. In particular, iPSC-derived neural cells permit a direct comparison of disease-relevant molecular pathways in neurons and glia derived from patients and healthy individuals. A prerequisite for such comparative studies are robust protocols that efficiently yield standardized populations of neural cell types. Here we show that long-term self-renewing neuroepithelial-like stem cells (lt-NES cells) derived from 3 hESC and 6 iPSC lines in two independent laboratories exhibit consistent characteristics including i) continuous expandability in the presence of FGF2 and EGF; ii) stable neuronal and glial differentiation competence; iii) characteristic transcription factor profile; iv) hindbrain specification amenable to regional patterning; v) capacity to generate functionally mature human neurons. We further show that lt-NES cells are developmentally distinct from fetal tissue-derived radial glia-like stem cells. We propose that lt-NES cells provide an interesting tool for studying human neurodevelopment and may serve as a standard system to facilitate comparative analyses of hESC and hiPSC-derived neural cells from control and diseased genetic backgrounds.
(Less)
- author
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cell Differentiation/drug effects, Cell Line, Cell Proliferation/drug effects, Cluster Analysis, Epidermal Growth Factor/pharmacology, Fibroblast Growth Factor 2/pharmacology, Fluorescent Antibody Technique, Gene Expression Profiling, Humans, Induced Pluripotent Stem Cells/cytology, Neural Stem Cells/cytology, Neuroepithelial Cells/cytology, Neuroglia/cytology, Neurons/cytology, Oligonucleotide Array Sequence Analysis, Pluripotent Stem Cells/cytology, Reverse Transcriptase Polymerase Chain Reaction, Transcription Factors/genetics
- in
- PLoS ONE
- volume
- 7
- issue
- 1
- article number
- e29597
- pages
- 13 pages
- publisher
- Public Library of Science (PLoS)
- external identifiers
-
- pmid:22272239
- scopus:84855853673
- ISSN
- 1932-6203
- DOI
- 10.1371/journal.pone.0029597
- language
- English
- LU publication?
- no
- id
- ad09c18f-ae2d-48f0-9eb4-611557518cdc
- date added to LUP
- 2021-08-10 13:40:16
- date last changed
- 2024-06-16 16:50:30
@article{ad09c18f-ae2d-48f0-9eb4-611557518cdc,
abstract = {{<p>Human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC) provide new prospects for studying human neurodevelopment and modeling neurological disease. In particular, iPSC-derived neural cells permit a direct comparison of disease-relevant molecular pathways in neurons and glia derived from patients and healthy individuals. A prerequisite for such comparative studies are robust protocols that efficiently yield standardized populations of neural cell types. Here we show that long-term self-renewing neuroepithelial-like stem cells (lt-NES cells) derived from 3 hESC and 6 iPSC lines in two independent laboratories exhibit consistent characteristics including i) continuous expandability in the presence of FGF2 and EGF; ii) stable neuronal and glial differentiation competence; iii) characteristic transcription factor profile; iv) hindbrain specification amenable to regional patterning; v) capacity to generate functionally mature human neurons. We further show that lt-NES cells are developmentally distinct from fetal tissue-derived radial glia-like stem cells. We propose that lt-NES cells provide an interesting tool for studying human neurodevelopment and may serve as a standard system to facilitate comparative analyses of hESC and hiPSC-derived neural cells from control and diseased genetic backgrounds.</p>}},
author = {{Falk, Anna and Koch, Philipp and Kesavan, Jaideep and Takashima, Yasuhiro and Ladewig, Julia and Alexander, Michael and Wiskow, Ole and Tailor, Jignesh and Trotter, Matthew and Pollard, Steven and Smith, Austin and Brüstle, Oliver}},
issn = {{1932-6203}},
keywords = {{Cell Differentiation/drug effects; Cell Line; Cell Proliferation/drug effects; Cluster Analysis; Epidermal Growth Factor/pharmacology; Fibroblast Growth Factor 2/pharmacology; Fluorescent Antibody Technique; Gene Expression Profiling; Humans; Induced Pluripotent Stem Cells/cytology; Neural Stem Cells/cytology; Neuroepithelial Cells/cytology; Neuroglia/cytology; Neurons/cytology; Oligonucleotide Array Sequence Analysis; Pluripotent Stem Cells/cytology; Reverse Transcriptase Polymerase Chain Reaction; Transcription Factors/genetics}},
language = {{eng}},
number = {{1}},
publisher = {{Public Library of Science (PLoS)}},
series = {{PLoS ONE}},
title = {{Capture of neuroepithelial-like stem cells from pluripotent stem cells provides a versatile system for in vitro production of human neurons}},
url = {{https://lup.lub.lu.se/search/files/101077195/Capture_of_Neuroepithelial_Like_Stem_Cells.pdf}},
doi = {{10.1371/journal.pone.0029597}},
volume = {{7}},
year = {{2012}},
}