Functional recovery of the germ line following splicing collapse
Cao, Wei ; Tran, Christopher ; Archer, Stuart K ; Gopal, Sandeep LU
and Pocock, Roger
(2022)
In Cell Death and Differentiation
29(4).
p.772-787
- Abstract
Splicing introns from precursor-messenger RNA (pre-mRNA) transcripts is essential for translating functional proteins. Here, we
report that the previously uncharacterized Caenorhabditis elegans protein MOG-7 acts as a pre-mRNA splicing factor. Depleting
MOG-7 from the C. elegans germ line causes intron retention in most germline-expressed genes, impeding the germ cell cycle, and
causing defects in nuclear morphology, germ cell identity and sterility. Despite the deleterious consequences caused by MOG-7
loss, the adult germ line can functionally recover to produce viable and fertile progeny when MOG-7 is restored. Germline recovery
is dependent on a burst of apoptosis that likely clears defective germ cells, and... (More)Splicing introns from precursor-messenger RNA (pre-mRNA) transcripts is essential for translating functional proteins. Here, we
(Less)
report that the previously uncharacterized Caenorhabditis elegans protein MOG-7 acts as a pre-mRNA splicing factor. Depleting
MOG-7 from the C. elegans germ line causes intron retention in most germline-expressed genes, impeding the germ cell cycle, and
causing defects in nuclear morphology, germ cell identity and sterility. Despite the deleterious consequences caused by MOG-7
loss, the adult germ line can functionally recover to produce viable and fertile progeny when MOG-7 is restored. Germline recovery
is dependent on a burst of apoptosis that likely clears defective germ cells, and viable gametes generated from the proliferation of
germ cells in the progenitor zone. Together, these findings reveal that MOG-7 is essential for germ cell development, and that the
germ line can functionally recover after a collapse in RNA splicing.
- author
- Cao, Wei
; Tran, Christopher
; Archer, Stuart K
; Gopal, Sandeep
LU
and Pocock, Roger
- publishing date
- 2022
- type
- Contribution to journal
- publication status
- published
- in
- Cell Death and Differentiation
- volume
- 29
- issue
- 4
- pages
- 772 - 787
- publisher
- Nature Publishing Group
- external identifiers
-
- scopus:85117165010
- pmid:34663906
- ISSN
- 1350-9047
- DOI
- 10.1038/s41418-021-00891-z
- language
- English
- LU publication?
- no
- additional info
- © 2021. The Author(s), under exclusive licence to ADMC Associazione Differenziamento e Morte Cellulare.
- id
- ad09ed7c-f64d-40b6-a529-4fc534572108
- date added to LUP
- 2021-10-25 12:51:21
- date last changed
- 2024-06-15 19:04:14
@article{ad09ed7c-f64d-40b6-a529-4fc534572108,
abstract = {{<p>Splicing introns from precursor-messenger RNA (pre-mRNA) transcripts is essential for translating functional proteins. Here, we<br>
report that the previously uncharacterized Caenorhabditis elegans protein MOG-7 acts as a pre-mRNA splicing factor. Depleting<br>
MOG-7 from the C. elegans germ line causes intron retention in most germline-expressed genes, impeding the germ cell cycle, and<br>
causing defects in nuclear morphology, germ cell identity and sterility. Despite the deleterious consequences caused by MOG-7<br>
loss, the adult germ line can functionally recover to produce viable and fertile progeny when MOG-7 is restored. Germline recovery<br>
is dependent on a burst of apoptosis that likely clears defective germ cells, and viable gametes generated from the proliferation of<br>
germ cells in the progenitor zone. Together, these findings reveal that MOG-7 is essential for germ cell development, and that the<br>
germ line can functionally recover after a collapse in RNA splicing.</p>}},
author = {{Cao, Wei and Tran, Christopher and Archer, Stuart K and Gopal, Sandeep and Pocock, Roger}},
issn = {{1350-9047}},
language = {{eng}},
number = {{4}},
pages = {{772--787}},
publisher = {{Nature Publishing Group}},
series = {{Cell Death and Differentiation}},
title = {{Functional recovery of the germ line following splicing collapse}},
url = {{http://dx.doi.org/10.1038/s41418-021-00891-z}},
doi = {{10.1038/s41418-021-00891-z}},
volume = {{29}},
year = {{2022}},
}