Loss of size-selectivity at histamine-induced exudation of plasma proteins in atopic nasal airways.
(2002) In Clinical Physiology and Functional Imaging 22(1). p.28-31- Abstract
- Plasma proteins occur in the airway lumen in inflammatory airway diseases. This study tests the hypothesis that airway microvascular-epithelial exudation of plasma proteins, as induced by a non-injurious inflammatory mediator, is characterized by loss of size-selectivity. Using a nasal pool-device, the nasal mucosa of 10 allergic individuals, without current disease, was sequentially exposed to saline and histamine (40 and 400 microg ml(-1)). Nasal lavage fluid and blood-levels of albumin (69 kD) and alpha2-macroglobulin (720 kD) were determined. Histamine produced concentration-dependent exudation of albumin and alpha2-macroglobulin. The albumin/alpha2-macroglobulin concentration ratio of the saline lavage fluid (baseline) was 40+/-19.... (More)
- Plasma proteins occur in the airway lumen in inflammatory airway diseases. This study tests the hypothesis that airway microvascular-epithelial exudation of plasma proteins, as induced by a non-injurious inflammatory mediator, is characterized by loss of size-selectivity. Using a nasal pool-device, the nasal mucosa of 10 allergic individuals, without current disease, was sequentially exposed to saline and histamine (40 and 400 microg ml(-1)). Nasal lavage fluid and blood-levels of albumin (69 kD) and alpha2-macroglobulin (720 kD) were determined. Histamine produced concentration-dependent exudation of albumin and alpha2-macroglobulin. The albumin/alpha2-macroglobulin concentration ratio of the saline lavage fluid (baseline) was 40+/-19. However, at the histamine challenges the ratios were 25+/-3 and 22+/-2, respectively, which did not differ from that of circulating plasma (22+/-2). We conclude that there is minor and size-selective luminal entry of plasma proteins at baseline. However, at concentration-dependent exudative responses to histamine, plasma proteins enter the airway lumen without being sieved. These data indicate that inflammatory stimulus-induced extravasation, lamina propria distribution and paracellular epithelial passage of plasma occur with minimal size-selectivity. Inferentially, the full immunological capacity of plasma proteins may readily be made available at the surface of human intact airway mucosa. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/108117
- author
- Greiff, Lennart LU ; Andersson, Morgan LU ; Erjefält, Jonas LU ; Svensson, Christer LU and Persson, Carl LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Dose-Response Relationship, Drug, Histamine : administration & dosage, Histamine : pharmacology, Human, Hypersensitivity : metabolism, Nasal Lavage Fluid : chemistry, Nasal Mucosa : drug effects, alpha-Macroglobulins : metabolism, Non-U.S. Gov't, Support, Serum Albumin : metabolism, Nasal Mucosa : metabolism, Permeability, Blood Proteins : metabolism, Adult
- in
- Clinical Physiology and Functional Imaging
- volume
- 22
- issue
- 1
- pages
- 28 - 31
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000174885800008
- pmid:12003096
- scopus:0036246738
- ISSN
- 1475-0961
- DOI
- 10.1046/j.1475-097X.2002.00390.x
- language
- English
- LU publication?
- yes
- id
- ad0c6e2f-2089-4ef0-bd99-bb5ba6b1f976 (old id 108117)
- date added to LUP
- 2016-04-01 11:42:33
- date last changed
- 2022-04-28 18:49:51
@article{ad0c6e2f-2089-4ef0-bd99-bb5ba6b1f976,
abstract = {{Plasma proteins occur in the airway lumen in inflammatory airway diseases. This study tests the hypothesis that airway microvascular-epithelial exudation of plasma proteins, as induced by a non-injurious inflammatory mediator, is characterized by loss of size-selectivity. Using a nasal pool-device, the nasal mucosa of 10 allergic individuals, without current disease, was sequentially exposed to saline and histamine (40 and 400 microg ml(-1)). Nasal lavage fluid and blood-levels of albumin (69 kD) and alpha2-macroglobulin (720 kD) were determined. Histamine produced concentration-dependent exudation of albumin and alpha2-macroglobulin. The albumin/alpha2-macroglobulin concentration ratio of the saline lavage fluid (baseline) was 40+/-19. However, at the histamine challenges the ratios were 25+/-3 and 22+/-2, respectively, which did not differ from that of circulating plasma (22+/-2). We conclude that there is minor and size-selective luminal entry of plasma proteins at baseline. However, at concentration-dependent exudative responses to histamine, plasma proteins enter the airway lumen without being sieved. These data indicate that inflammatory stimulus-induced extravasation, lamina propria distribution and paracellular epithelial passage of plasma occur with minimal size-selectivity. Inferentially, the full immunological capacity of plasma proteins may readily be made available at the surface of human intact airway mucosa.}},
author = {{Greiff, Lennart and Andersson, Morgan and Erjefält, Jonas and Svensson, Christer and Persson, Carl}},
issn = {{1475-0961}},
keywords = {{Dose-Response Relationship; Drug; Histamine : administration & dosage; Histamine : pharmacology; Human; Hypersensitivity : metabolism; Nasal Lavage Fluid : chemistry; Nasal Mucosa : drug effects; alpha-Macroglobulins : metabolism; Non-U.S. Gov't; Support; Serum Albumin : metabolism; Nasal Mucosa : metabolism; Permeability; Blood Proteins : metabolism; Adult}},
language = {{eng}},
number = {{1}},
pages = {{28--31}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Clinical Physiology and Functional Imaging}},
title = {{Loss of size-selectivity at histamine-induced exudation of plasma proteins in atopic nasal airways.}},
url = {{https://lup.lub.lu.se/search/files/2605586/623606.pdf}},
doi = {{10.1046/j.1475-097X.2002.00390.x}},
volume = {{22}},
year = {{2002}},
}