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Intestinal preservation injury : A comparison between rat, porcine and human intestines

Søfteland, John Mackay; Casselbrant, Anna; Biglarnia, Ali Reza; Linders, Johan LU ; Hellström, Mats; Pesce, Antonio; Padma, Arvind Manikantan; Jiga, Lucian Petru; Hoinoiu, Bogdan and Ionac, Mihai, et al. (2019) In International Journal of Molecular Sciences 20(13).
Abstract

Advanced preservation injury (PI) after intestinal transplantation has deleterious short-and long-term effects and constitutes a major research topic. Logistics and costs favor rodent studies, whereas clinical translation mandates studies in larger animals or using human material. Despite diverging reports, no direct comparison between the development of intestinal PI in rats, pigs, and humans is available. We compared the development of PI in rat, porcine, and human intestines. Intestinal procurement and cold storage (CS) using histidine–tryptophan–ketoglutarate solution was performed in rats, pigs, and humans. Tissue samples were obtained after 8, 14, and 24 h of CS), and PI was assessed morphologically and at the molecular level... (More)

Advanced preservation injury (PI) after intestinal transplantation has deleterious short-and long-term effects and constitutes a major research topic. Logistics and costs favor rodent studies, whereas clinical translation mandates studies in larger animals or using human material. Despite diverging reports, no direct comparison between the development of intestinal PI in rats, pigs, and humans is available. We compared the development of PI in rat, porcine, and human intestines. Intestinal procurement and cold storage (CS) using histidine–tryptophan–ketoglutarate solution was performed in rats, pigs, and humans. Tissue samples were obtained after 8, 14, and 24 h of CS), and PI was assessed morphologically and at the molecular level (cleaved caspase-3, zonula occludens, claudin-3 and 4, tricellulin, occludin, cytokeratin-8) using immunohistochemistry and Western blot. Intestinal PI developed slower in pigs compared to rats and humans. Tissue injury and apoptosis were significantly higher in rats. Tight junction proteins showed quantitative and qualitative changes differing between species. Significant interspecies differences exist between rats, pigs, and humans regarding intestinal PI progression at tissue and molecular levels. These differences should be taken into account both with regards to study design and the interpretation of findings when relating them to the clinical setting.

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publishing date
type
Contribution to journal
publication status
published
subject
keywords
Intestinal mucosa, Intestine, Ischemia, Organ preservation, Tight junctions, Transplantation
in
International Journal of Molecular Sciences
volume
20
issue
13
publisher
MOLECULAR DIVERSITY PRESERVATION INT
external identifiers
  • scopus:85068593122
ISSN
1661-6596
DOI
10.3390/ijms20133135
language
English
LU publication?
no
id
ad32c48d-9053-4316-852b-5c93797294f2
date added to LUP
2019-07-16 16:25:58
date last changed
2019-08-14 04:41:52
@article{ad32c48d-9053-4316-852b-5c93797294f2,
  abstract     = {<p>Advanced preservation injury (PI) after intestinal transplantation has deleterious short-and long-term effects and constitutes a major research topic. Logistics and costs favor rodent studies, whereas clinical translation mandates studies in larger animals or using human material. Despite diverging reports, no direct comparison between the development of intestinal PI in rats, pigs, and humans is available. We compared the development of PI in rat, porcine, and human intestines. Intestinal procurement and cold storage (CS) using histidine–tryptophan–ketoglutarate solution was performed in rats, pigs, and humans. Tissue samples were obtained after 8, 14, and 24 h of CS), and PI was assessed morphologically and at the molecular level (cleaved caspase-3, zonula occludens, claudin-3 and 4, tricellulin, occludin, cytokeratin-8) using immunohistochemistry and Western blot. Intestinal PI developed slower in pigs compared to rats and humans. Tissue injury and apoptosis were significantly higher in rats. Tight junction proteins showed quantitative and qualitative changes differing between species. Significant interspecies differences exist between rats, pigs, and humans regarding intestinal PI progression at tissue and molecular levels. These differences should be taken into account both with regards to study design and the interpretation of findings when relating them to the clinical setting.</p>},
  articleno    = {3135},
  author       = {Søfteland, John Mackay and Casselbrant, Anna and Biglarnia, Ali Reza and Linders, Johan and Hellström, Mats and Pesce, Antonio and Padma, Arvind Manikantan and Jiga, Lucian Petru and Hoinoiu, Bogdan and Ionac, Mihai and Oltean, Mihai},
  issn         = {1661-6596},
  keyword      = {Intestinal mucosa,Intestine,Ischemia,Organ preservation,Tight junctions,Transplantation},
  language     = {eng},
  month        = {07},
  number       = {13},
  publisher    = {MOLECULAR DIVERSITY PRESERVATION INT},
  series       = {International Journal of Molecular Sciences},
  title        = {Intestinal preservation injury : A comparison between rat, porcine and human intestines},
  url          = {http://dx.doi.org/10.3390/ijms20133135},
  volume       = {20},
  year         = {2019},
}