Fluorescence microscopy of nanochannel-confined DNA
Persson, Fredrik LU ; Westerlund, Fredrik and Tegenfeldt, Jonas O. LU
(2011)
In Methods in Molecular Biology
783.
p.159-179
- Abstract
Stretching of DNA in nanoscale confinement allows for direct visualization of the genetic contents of the DNA on the single DNA molecule level. DNA stretched in nanoscale confinement also allows for studies of DNA-protein interactions and DNA polymer physics in confined environments. This chapter describes the basic steps to fabricate the nanostructures, to perform the experiments, and to analyze the data.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/ad66d9ba-52bb-41ea-bdb4-a677c2319128
- author
- Persson, Fredrik
LU
; Westerlund, Fredrik
and Tegenfeldt, Jonas O.
LU
- organization
- publishing date
- 2011-10-24
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- DNA, Fluorescence, Nanochannels, Single molecule
- host publication
- Single Molecule Analysis : Methods and Protocols - Methods and Protocols
- series title
- Methods in Molecular Biology
- editor
- Peterman, Erwin J.G. and Wuite, Gijs J.L.
- volume
- 783
- pages
- 21 pages
- external identifiers
-
- pmid:21909888
- scopus:80054721557
- ISSN
- 1064-3745
- ISBN
- 9781617792816
- DOI
- 10.1007/978-1-61779-282-3_9
- language
- English
- LU publication?
- yes
- id
- ad66d9ba-52bb-41ea-bdb4-a677c2319128
- date added to LUP
- 2018-10-20 08:58:03
- date last changed
- 2024-01-15 04:37:57
@inbook{ad66d9ba-52bb-41ea-bdb4-a677c2319128,
abstract = {{<p>Stretching of DNA in nanoscale confinement allows for direct visualization of the genetic contents of the DNA on the single DNA molecule level. DNA stretched in nanoscale confinement also allows for studies of DNA-protein interactions and DNA polymer physics in confined environments. This chapter describes the basic steps to fabricate the nanostructures, to perform the experiments, and to analyze the data.</p>}},
author = {{Persson, Fredrik and Westerlund, Fredrik and Tegenfeldt, Jonas O.}},
booktitle = {{Single Molecule Analysis : Methods and Protocols}},
editor = {{Peterman, Erwin J.G. and Wuite, Gijs J.L.}},
isbn = {{9781617792816}},
issn = {{1064-3745}},
keywords = {{DNA; Fluorescence; Nanochannels; Single molecule}},
language = {{eng}},
month = {{10}},
pages = {{159--179}},
series = {{Methods in Molecular Biology}},
title = {{Fluorescence microscopy of nanochannel-confined DNA}},
url = {{http://dx.doi.org/10.1007/978-1-61779-282-3_9}},
doi = {{10.1007/978-1-61779-282-3_9}},
volume = {{783}},
year = {{2011}},
}