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Fluorescence microscopy of nanochannel-confined DNA

Persson, Fredrik LU ; Westerlund, Fredrik and Tegenfeldt, Jonas O. LU (2011) In Methods in Molecular Biology 783. p.159-179
Abstract

Stretching of DNA in nanoscale confinement allows for direct visualization of the genetic contents of the DNA on the single DNA molecule level. DNA stretched in nanoscale confinement also allows for studies of DNA-protein interactions and DNA polymer physics in confined environments. This chapter describes the basic steps to fabricate the nanostructures, to perform the experiments, and to analyze the data.

Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
DNA, Fluorescence, Nanochannels, Single molecule
host publication
Single Molecule Analysis
series title
Methods in Molecular Biology
editor
Peterman, Erwin J.G.; Wuite, Gijs J.L.; and
volume
783
pages
21 pages
external identifiers
  • scopus:80054721557
ISSN
1064-3745
ISBN
9781617792816
DOI
10.1007/978-1-61779-282-3_9
language
English
LU publication?
yes
id
ad66d9ba-52bb-41ea-bdb4-a677c2319128
date added to LUP
2018-10-20 08:58:03
date last changed
2019-04-19 10:17:43
@inbook{ad66d9ba-52bb-41ea-bdb4-a677c2319128,
  abstract     = {<p>Stretching of DNA in nanoscale confinement allows for direct visualization of the genetic contents of the DNA on the single DNA molecule level. DNA stretched in nanoscale confinement also allows for studies of DNA-protein interactions and DNA polymer physics in confined environments. This chapter describes the basic steps to fabricate the nanostructures, to perform the experiments, and to analyze the data.</p>},
  author       = {Persson, Fredrik and Westerlund, Fredrik and Tegenfeldt, Jonas O.},
  editor       = {Peterman, Erwin J.G. and Wuite, Gijs J.L.},
  isbn         = {9781617792816},
  issn         = {1064-3745},
  keyword      = {DNA,Fluorescence,Nanochannels,Single molecule},
  language     = {eng},
  month        = {10},
  pages        = {159--179},
  series       = {Methods in Molecular Biology},
  title        = {Fluorescence microscopy of nanochannel-confined DNA},
  url          = {http://dx.doi.org/10.1007/978-1-61779-282-3_9},
  volume       = {783},
  year         = {2011},
}