In situ hybridization histochemistry.
Kokaia, Z. LU
(2001)
In Current Protocols in Toxicology
5(1).
p.1-13
- Abstract
This unit describes two methods of in situ hybridization: one uses an 35S-labeled oligonucleotide probe and the other uses a digoxigenin-labeled oligonucleotide probe on frozen, cryostat-sectioned samples. These methods allow detection of the physical distribution and expression levels of target mRNA. Protocols are also included for labeling the probes and preparing the sample material.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/add6f592-3af4-45df-aeeb-014b04c0e9d2
- author
- Kokaia, Z.
LU
- publishing date
- 2001-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Current Protocols in Toxicology
- volume
- 5
- issue
- 1
- pages
- 1 - 13
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:84875009920
- pmid:23045047
- DOI
- 10.1002/0471140856.tx0207s05
- language
- English
- LU publication?
- no
- id
- add6f592-3af4-45df-aeeb-014b04c0e9d2
- date added to LUP
- 2019-09-03 16:58:42
- date last changed
- 2024-01-01 18:42:13
@article{add6f592-3af4-45df-aeeb-014b04c0e9d2,
abstract = {{<p>This unit describes two methods of in situ hybridization: one uses an <sup>35</sup>S-labeled oligonucleotide probe and the other uses a digoxigenin-labeled oligonucleotide probe on frozen, cryostat-sectioned samples. These methods allow detection of the physical distribution and expression levels of target mRNA. Protocols are also included for labeling the probes and preparing the sample material.</p>}},
author = {{Kokaia, Z.}},
language = {{eng}},
month = {{01}},
number = {{1}},
pages = {{1--13}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Current Protocols in Toxicology}},
title = {{In situ hybridization histochemistry.}},
url = {{http://dx.doi.org/10.1002/0471140856.tx0207s05}},
doi = {{10.1002/0471140856.tx0207s05}},
volume = {{5}},
year = {{2001}},
}