A prospective analysis of islet-cell cytotoxic antibodies in insulin-dependent diabetic children. Transient effects of plasmapheresis
(1984) In Diabetes 33(3). p.224-228- Abstract
We determined the effects of plasmapheresis on cytotoxic antibodies to islet cells in 10 children (aged 11-16 yr) with newly diagnosed insulin-dependent diabetes mellitus (IDDM), as well as the plasma levels of antibodies over the next 30 mo and their relation to serum C-peptide concentrations. Complement-dependent, antibody-mediated cytotoxicity (C'AMC) in plasma was measured in a 51Cr release assay using monolayers of the rat islet cell line RINm5F. Cytotoxic antibodices decreased in most IDDM subjects treated by plasmapheresis four times within 2 wk of diagnosis; however, the decreases were small and lasted less than 2-3 days. Thus, both before and after plasmapheresis, 7 of the 10 IDDM children were C'AMC-positive... (More)
We determined the effects of plasmapheresis on cytotoxic antibodies to islet cells in 10 children (aged 11-16 yr) with newly diagnosed insulin-dependent diabetes mellitus (IDDM), as well as the plasma levels of antibodies over the next 30 mo and their relation to serum C-peptide concentrations. Complement-dependent, antibody-mediated cytotoxicity (C'AMC) in plasma was measured in a 51Cr release assay using monolayers of the rat islet cell line RINm5F. Cytotoxic antibodices decreased in most IDDM subjects treated by plasmapheresis four times within 2 wk of diagnosis; however, the decreases were small and lasted less than 2-3 days. Thus, both before and after plasmapheresis, 7 of the 10 IDDM children were C'AMC-positive (51Cr release > 2 SD above mean for 13 healthy children). After 18-30 mo, only 2 of the original 7 IDDM children with C'AMC-positive plasmas were still positive, and 1 of the original 3 IDDM children without significant cytotoxicity had become positive. Meal-stimulated serum C-peptide responses, measured from diagnosis to 18-30 mo later, did not correlate with C'AMC values. We conclude that (1) plasmapheresis has only transient effects on islet-cytotoxic antibody levels in children with IDDM; (2) these antibodies decrease in most, but not all, subjects over the first 18 mo after diagnosis; and (3) the level of cytotoxicity antibodies in IDDM plasma at diagnosis has no predictive effect on residual B-cell function.
(Less)
- author
- Rabinovitch, A.
; MacKay, P.
; Ludvigsson, J.
and Lernmark, A.
LU
- publishing date
- 1984-01-01
- type
- Contribution to journal
- publication status
- published
- in
- Diabetes
- volume
- 33
- issue
- 3
- pages
- 5 pages
- publisher
- American Diabetes Association Inc.
- external identifiers
-
- scopus:0021349363
- pmid:6365659
- ISSN
- 0012-1797
- DOI
- 10.2337/diab.33.3.224
- language
- English
- LU publication?
- no
- id
- adf56648-950a-46b5-b0f2-677ddfa319b3
- date added to LUP
- 2019-09-16 12:40:15
- date last changed
- 2024-03-13 08:22:54
@article{adf56648-950a-46b5-b0f2-677ddfa319b3, abstract = {{<p>We determined the effects of plasmapheresis on cytotoxic antibodies to islet cells in 10 children (aged 11-16 yr) with newly diagnosed insulin-dependent diabetes mellitus (IDDM), as well as the plasma levels of antibodies over the next 30 mo and their relation to serum C-peptide concentrations. Complement-dependent, antibody-mediated cytotoxicity (C'AMC) in plasma was measured in a <sup>51</sup>Cr release assay using monolayers of the rat islet cell line RINm5F. Cytotoxic antibodices decreased in most IDDM subjects treated by plasmapheresis four times within 2 wk of diagnosis; however, the decreases were small and lasted less than 2-3 days. Thus, both before and after plasmapheresis, 7 of the 10 IDDM children were C'AMC-positive (<sup>51</sup>Cr release > 2 SD above mean for 13 healthy children). After 18-30 mo, only 2 of the original 7 IDDM children with C'AMC-positive plasmas were still positive, and 1 of the original 3 IDDM children without significant cytotoxicity had become positive. Meal-stimulated serum C-peptide responses, measured from diagnosis to 18-30 mo later, did not correlate with C'AMC values. We conclude that (1) plasmapheresis has only transient effects on islet-cytotoxic antibody levels in children with IDDM; (2) these antibodies decrease in most, but not all, subjects over the first 18 mo after diagnosis; and (3) the level of cytotoxicity antibodies in IDDM plasma at diagnosis has no predictive effect on residual B-cell function.</p>}}, author = {{Rabinovitch, A. and MacKay, P. and Ludvigsson, J. and Lernmark, A.}}, issn = {{0012-1797}}, language = {{eng}}, month = {{01}}, number = {{3}}, pages = {{224--228}}, publisher = {{American Diabetes Association Inc.}}, series = {{Diabetes}}, title = {{A prospective analysis of islet-cell cytotoxic antibodies in insulin-dependent diabetic children. Transient effects of plasmapheresis}}, url = {{http://dx.doi.org/10.2337/diab.33.3.224}}, doi = {{10.2337/diab.33.3.224}}, volume = {{33}}, year = {{1984}}, }