Thylakoid membrane reorganizations revealed by small-angle neutron scattering of Monstera deliciosa leaves associated with non-photochemical quenching
(2020) In Open biology 10(9). p.200144-200144- Abstract
Non-photochemical quenching (NPQ) is an important photoprotective mechanism in plants and algae. Although the process is extensively studied, little is known about its relationship with ultrastructural changes of the thylakoid membranes. In order to better understand this relationship, we studied the effects of illumination on the organization of thylakoid membranes in Monstera deliciosa leaves. This evergreen species is known to exhibit very large NPQ and to possess giant grana with dozens of stacked thylakoids. It is thus ideally suited for small-angle neutron scattering measurements (SANS)-a non-invasive technique, which is capable of providing spatially and statistically averaged information on the periodicity of the thylakoid... (More)
Non-photochemical quenching (NPQ) is an important photoprotective mechanism in plants and algae. Although the process is extensively studied, little is known about its relationship with ultrastructural changes of the thylakoid membranes. In order to better understand this relationship, we studied the effects of illumination on the organization of thylakoid membranes in Monstera deliciosa leaves. This evergreen species is known to exhibit very large NPQ and to possess giant grana with dozens of stacked thylakoids. It is thus ideally suited for small-angle neutron scattering measurements (SANS)-a non-invasive technique, which is capable of providing spatially and statistically averaged information on the periodicity of the thylakoid membranes and their rapid reorganizations in vivo. We show that NPQ-inducing illumination causes a strong decrease in the periodic order of granum thylakoid membranes. Development of NPQ and light-induced ultrastructural changes, as well as the relaxation processes, follow similar kinetic patterns. Surprisingly, whereas NPQ is suppressed by diuron, it impedes only the relaxation of the structural changes and not its formation, suggesting that structural changes do not cause but enable NPQ. We also demonstrate that the diminishment of SANS peak does not originate from light-induced redistribution and reorientation of chloroplasts inside the cells.
(Less)
- author
- publishing date
- 2020-09-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- chloroplast thylakoid membranes, grana, lamellar repeat distance, non-photochemical quenching, small-angle neutron scattering
- in
- Open biology
- volume
- 10
- issue
- 9
- pages
- 1 pages
- publisher
- Royal Society Publishing
- external identifiers
-
- pmid:32931722
- scopus:85091051616
- ISSN
- 2046-2441
- DOI
- 10.1098/rsob.200144
- language
- English
- LU publication?
- no
- id
- adf92ebc-bf07-4926-8ac5-759c0416f383
- date added to LUP
- 2020-10-12 09:58:59
- date last changed
- 2024-08-22 04:48:12
@article{adf92ebc-bf07-4926-8ac5-759c0416f383,
abstract = {{<p>Non-photochemical quenching (NPQ) is an important photoprotective mechanism in plants and algae. Although the process is extensively studied, little is known about its relationship with ultrastructural changes of the thylakoid membranes. In order to better understand this relationship, we studied the effects of illumination on the organization of thylakoid membranes in Monstera deliciosa leaves. This evergreen species is known to exhibit very large NPQ and to possess giant grana with dozens of stacked thylakoids. It is thus ideally suited for small-angle neutron scattering measurements (SANS)-a non-invasive technique, which is capable of providing spatially and statistically averaged information on the periodicity of the thylakoid membranes and their rapid reorganizations in vivo. We show that NPQ-inducing illumination causes a strong decrease in the periodic order of granum thylakoid membranes. Development of NPQ and light-induced ultrastructural changes, as well as the relaxation processes, follow similar kinetic patterns. Surprisingly, whereas NPQ is suppressed by diuron, it impedes only the relaxation of the structural changes and not its formation, suggesting that structural changes do not cause but enable NPQ. We also demonstrate that the diminishment of SANS peak does not originate from light-induced redistribution and reorientation of chloroplasts inside the cells.</p>}},
author = {{Ünnep, Renáta and Paul, Suman and Zsiros, Ottó and Kovács, László and Székely, Noémi K. and Steinbach, Gábor and Appavou, Marie Sousai and Porcar, Lionel and Holzwarth, Alfred R. and Garab, Győző and Nagy, Gergely}},
issn = {{2046-2441}},
keywords = {{chloroplast thylakoid membranes; grana; lamellar repeat distance; non-photochemical quenching; small-angle neutron scattering}},
language = {{eng}},
month = {{09}},
number = {{9}},
pages = {{200144--200144}},
publisher = {{Royal Society Publishing}},
series = {{Open biology}},
title = {{Thylakoid membrane reorganizations revealed by small-angle neutron scattering of Monstera deliciosa leaves associated with non-photochemical quenching}},
url = {{http://dx.doi.org/10.1098/rsob.200144}},
doi = {{10.1098/rsob.200144}},
volume = {{10}},
year = {{2020}},
}