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Identification of SLPI (Secretory leukocyte protease inhibitor) in human mast cells using immunohistochemistry and in situ hybridisation

Westin, Ulla LU ; Polling, Åsa ; Ljungkrantz, Irena and Ohlsson, Kjell (1999) In Biological Chemistry 380(4). p.489-493
Abstract

Recently interest has been focused on secretory leucocyte protease inhibitor (SLPI) and its role in immediate hypersensitive reactions, possibly by inhibiting mast cell chymase. The purpose of this investigation was to show whether or not SLPI is produced in mast cells. Double-immunolabelling revealed that SLPI coexists with mast cell tryptase (60%) and chymase (37%). On the other hand, in situ hybridisation studies demonstrated the expression of SLPI mRNA in all mast cells. The differences in results can be attributed to the fact that in situ hybridisation is a more sensitive method than immunohistochemistry. Hence, we conclude that SLPI is produced in human tonsillar mast cells.

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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
keywords
Chymase, Mast cell, Protease inhibitor, SLPI, Tryptase
in
Biological Chemistry
volume
380
issue
4
pages
489 - 493
publisher
De Gruyter
external identifiers
  • scopus:0032940249
  • pmid:10355635
ISSN
1431-6730
DOI
10.1515/BC.1999.063
language
English
LU publication?
yes
id
ae1110d6-b7fb-4db4-ae57-172d0fa42091
date added to LUP
2017-02-13 16:01:46
date last changed
2025-01-07 06:46:46
@article{ae1110d6-b7fb-4db4-ae57-172d0fa42091,
  abstract     = {{<p>Recently interest has been focused on secretory leucocyte protease inhibitor (SLPI) and its role in immediate hypersensitive reactions, possibly by inhibiting mast cell chymase. The purpose of this investigation was to show whether or not SLPI is produced in mast cells. Double-immunolabelling revealed that SLPI coexists with mast cell tryptase (60%) and chymase (37%). On the other hand, in situ hybridisation studies demonstrated the expression of SLPI mRNA in all mast cells. The differences in results can be attributed to the fact that in situ hybridisation is a more sensitive method than immunohistochemistry. Hence, we conclude that SLPI is produced in human tonsillar mast cells.</p>}},
  author       = {{Westin, Ulla and Polling, Åsa and Ljungkrantz, Irena and Ohlsson, Kjell}},
  issn         = {{1431-6730}},
  keywords     = {{Chymase; Mast cell; Protease inhibitor; SLPI; Tryptase}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{489--493}},
  publisher    = {{De Gruyter}},
  series       = {{Biological Chemistry}},
  title        = {{Identification of SLPI (Secretory leukocyte protease inhibitor) in human mast cells using immunohistochemistry and in situ hybridisation}},
  url          = {{http://dx.doi.org/10.1515/BC.1999.063}},
  doi          = {{10.1515/BC.1999.063}},
  volume       = {{380}},
  year         = {{1999}},
}