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Modeling the evolution of culture-adapted human embryonic stem cells

Olariu, Victor LU ; Harrison, Neil J.; Coca, Daniel; Gokhale, Paul J.; Baker, Duncan; Billings, Steve; Kadirkamanathan, Visakan and Andrews, Peter W. (2010) In Stem Cell Research 4(1). p.50-56
Abstract

The long-term culture of human embryonic stem (ES) cells is inevitably subject to evolution, since any mutant that arises with a growth advantage will be selectively amplified. However, the evolutionary influences of population size, mutation rate, and selection pressure are frequently overlooked. We have constructed a Monte Carlo simulation model to predict how changes in these factors can influence the appearance and spread of mutant ES cells, and verified its applicability by comparison with in vitro data. This simulation provides an estimate for the expected rate of generation of culture-adapted ES cells under different assumptions for the key parameters. In particular, it highlights the effect of population size, suggesting that... (More)

The long-term culture of human embryonic stem (ES) cells is inevitably subject to evolution, since any mutant that arises with a growth advantage will be selectively amplified. However, the evolutionary influences of population size, mutation rate, and selection pressure are frequently overlooked. We have constructed a Monte Carlo simulation model to predict how changes in these factors can influence the appearance and spread of mutant ES cells, and verified its applicability by comparison with in vitro data. This simulation provides an estimate for the expected rate of generation of culture-adapted ES cells under different assumptions for the key parameters. In particular, it highlights the effect of population size, suggesting that the maintenance of cells in small populations reduces the likelihood that abnormal cultures will develop.

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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Stem Cell Research
volume
4
issue
1
pages
7 pages
publisher
Elsevier
external identifiers
  • scopus:73149094719
ISSN
1873-5061
DOI
10.1016/j.scr.2009.09.001
language
English
LU publication?
no
id
ae3061c5-41c9-4ca5-9a6a-71060676298f
date added to LUP
2019-05-24 14:03:20
date last changed
2019-07-15 11:27:30
@article{ae3061c5-41c9-4ca5-9a6a-71060676298f,
  abstract     = {<p>The long-term culture of human embryonic stem (ES) cells is inevitably subject to evolution, since any mutant that arises with a growth advantage will be selectively amplified. However, the evolutionary influences of population size, mutation rate, and selection pressure are frequently overlooked. We have constructed a Monte Carlo simulation model to predict how changes in these factors can influence the appearance and spread of mutant ES cells, and verified its applicability by comparison with in vitro data. This simulation provides an estimate for the expected rate of generation of culture-adapted ES cells under different assumptions for the key parameters. In particular, it highlights the effect of population size, suggesting that the maintenance of cells in small populations reduces the likelihood that abnormal cultures will develop.</p>},
  author       = {Olariu, Victor and Harrison, Neil J. and Coca, Daniel and Gokhale, Paul J. and Baker, Duncan and Billings, Steve and Kadirkamanathan, Visakan and Andrews, Peter W.},
  issn         = {1873-5061},
  language     = {eng},
  number       = {1},
  pages        = {50--56},
  publisher    = {Elsevier},
  series       = {Stem Cell Research},
  title        = {Modeling the evolution of culture-adapted human embryonic stem cells},
  url          = {http://dx.doi.org/10.1016/j.scr.2009.09.001},
  volume       = {4},
  year         = {2010},
}