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Profily endometrialni exprese mRNA u vybranych matrix metaloproteinaz a tkanoveho inhibitoru metaloproteinaz-1

Pilka, Radovan LU ; Kudela, M ; Eriksson, P and Casslén, Bertil LU (2004) In Ceska gynekologie / Ceska lekarska spolecnost J. Ev. Purkyne 69(6). p.472-476
Abstract
OBJECTIVE: To examine the endometrial expression pattern of messenger RNA (mRNA) for selected matrix metalloproteinases (MMP) and tissue inhibitor of metalloproteinases-1 (TIMP-1). DESIGN: Experimental study. SETTING: Department of Obstetrics and gynecology of the Palacky University Medical School and University Hospital, Olomouc, Czech Republic, Department of Obsterics and Gynecology, University Hospital, Luna, Sweden. METHODS: We studied MMP-1, -3, -7, -10, -11, -12, -13, -14, -16, -26 and TIMP-1 mRNA in 39 normal endometrial samples obtained across the menstrual cycle. Based on histological examination, all specimens were classified according to an ideal 28 day menstrual cycle as early (n=8), mid (n=6) and late (n=7) proliferative... (More)
OBJECTIVE: To examine the endometrial expression pattern of messenger RNA (mRNA) for selected matrix metalloproteinases (MMP) and tissue inhibitor of metalloproteinases-1 (TIMP-1). DESIGN: Experimental study. SETTING: Department of Obstetrics and gynecology of the Palacky University Medical School and University Hospital, Olomouc, Czech Republic, Department of Obsterics and Gynecology, University Hospital, Luna, Sweden. METHODS: We studied MMP-1, -3, -7, -10, -11, -12, -13, -14, -16, -26 and TIMP-1 mRNA in 39 normal endometrial samples obtained across the menstrual cycle. Based on histological examination, all specimens were classified according to an ideal 28 day menstrual cycle as early (n=8), mid (n=6) and late (n=7) proliferative phase, early (n=4), mid (n=4) and late (n=8) secretory phase and menstrual (n=3) phase. mRNA extracted from frozen tissue samples was quantitated using real time PCR. RESULTS: Four distinct patterns of MMP mRNA expression were detected in cycling endometrium. MMP-1, -3, -10, and -12 were expressed predominantly in perimenstrual period, MMP-7 and -11 had highest levels in proliferative phase, MMP-13, -14 and -16 were expressed throughout the cycle and MMP-26 was found to be maximal in periovulatory period. Levels of TIMP-1 mRNA remained unchanged during the cycle. CONCLUSION: The specific endometrial expression profiles of MMPs during menstrual cycle point to their specific biologic roles during the cycle. MMP-26 exhibits a unique expression pattern. (Less)
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author
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organization
alternative title
Endometrial expression profiles of mRNA for selected matrix metalloproteinases and tissue inhibitor of metalloproteinases-1
publishing date
type
Contribution to journal
publication status
published
subject
in
Ceska gynekologie / Ceska lekarska spolecnost J. Ev. Purkyne
volume
69
issue
6
pages
472 - 476
publisher
Ceska Lekarska Spolecnost J. E. Purkyne
external identifiers
  • pmid:15633417
  • scopus:9644278158
ISSN
1210-7832
language
Czech
LU publication?
yes
id
ae807576-c7de-4554-87f1-61b73d49f9e4 (old id 1129629)
date added to LUP
2016-04-01 15:50:40
date last changed
2022-06-20 13:29:40
@article{ae807576-c7de-4554-87f1-61b73d49f9e4,
  abstract     = {{OBJECTIVE: To examine the endometrial expression pattern of messenger RNA (mRNA) for selected matrix metalloproteinases (MMP) and tissue inhibitor of metalloproteinases-1 (TIMP-1). DESIGN: Experimental study. SETTING: Department of Obstetrics and gynecology of the Palacky University Medical School and University Hospital, Olomouc, Czech Republic, Department of Obsterics and Gynecology, University Hospital, Luna, Sweden. METHODS: We studied MMP-1, -3, -7, -10, -11, -12, -13, -14, -16, -26 and TIMP-1 mRNA in 39 normal endometrial samples obtained across the menstrual cycle. Based on histological examination, all specimens were classified according to an ideal 28 day menstrual cycle as early (n=8), mid (n=6) and late (n=7) proliferative phase, early (n=4), mid (n=4) and late (n=8) secretory phase and menstrual (n=3) phase. mRNA extracted from frozen tissue samples was quantitated using real time PCR. RESULTS: Four distinct patterns of MMP mRNA expression were detected in cycling endometrium. MMP-1, -3, -10, and -12 were expressed predominantly in perimenstrual period, MMP-7 and -11 had highest levels in proliferative phase, MMP-13, -14 and -16 were expressed throughout the cycle and MMP-26 was found to be maximal in periovulatory period. Levels of TIMP-1 mRNA remained unchanged during the cycle. CONCLUSION: The specific endometrial expression profiles of MMPs during menstrual cycle point to their specific biologic roles during the cycle. MMP-26 exhibits a unique expression pattern.}},
  author       = {{Pilka, Radovan and Kudela, M and Eriksson, P and Casslén, Bertil}},
  issn         = {{1210-7832}},
  language     = {{cze}},
  number       = {{6}},
  pages        = {{472--476}},
  publisher    = {{Ceska Lekarska Spolecnost J. E. Purkyne}},
  series       = {{Ceska gynekologie / Ceska lekarska spolecnost J. Ev. Purkyne}},
  title        = {{Profily endometrialni exprese mRNA u vybranych matrix metaloproteinaz a tkanoveho inhibitoru metaloproteinaz-1}},
  volume       = {{69}},
  year         = {{2004}},
}