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Development of an immunoassay for glypressin, an N-terminal extended vasopressin analogue.

Ohlin, Mats LU orcid and Alkner, Ulf (1988) In J Immunoassay 9(1). p.19-36
Abstract
The development and evaluation of a radioimmunoassay for Nα-triglycyl-lysine8-vasopressin is described. The site of hapten conjugation of the immunogen has been controlled and the use of various radiolabelled tracers has been evaluated with special reference to the site of iodination. The most extensively studied antiserum showed specificity for the N-terminal triglycyl-extension as well as for several aminoacid residues of the vasopressin ring. It crossreacted 27%, 28% and 0.3% with Lys8-vasopressin, arg8-vasopressin and oxytocin respectively, and it was used to quantify triglycyl-lysine8-vasopressin in human plasma after SepPak C18 extraction. The sensitivity of the assay was 5 pg/tube with an intra-assay CV of 5–6% at 17 and 70 pg/tube.... (More)
The development and evaluation of a radioimmunoassay for Nα-triglycyl-lysine8-vasopressin is described. The site of hapten conjugation of the immunogen has been controlled and the use of various radiolabelled tracers has been evaluated with special reference to the site of iodination. The most extensively studied antiserum showed specificity for the N-terminal triglycyl-extension as well as for several aminoacid residues of the vasopressin ring. It crossreacted 27%, 28% and 0.3% with Lys8-vasopressin, arg8-vasopressin and oxytocin respectively, and it was used to quantify triglycyl-lysine8-vasopressin in human plasma after SepPak C18 extraction. The sensitivity of the assay was 5 pg/tube with an intra-assay CV of 5–6% at 17 and 70 pg/tube. The identity of the immunoreactivity was studied by reversed phase chromatography.

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author
and
publishing date
type
Contribution to journal
publication status
published
subject
in
J Immunoassay
volume
9
issue
1
pages
19 - 36
publisher
Marcel Dekker
external identifiers
  • scopus:0023863038
ISSN
0197-1522
DOI
10.1080/01971528808053208
language
English
LU publication?
no
id
ae86f70f-b0e3-42ef-8a3f-15d7334b083c
date added to LUP
2022-12-05 08:05:52
date last changed
2022-12-06 17:21:27
@article{ae86f70f-b0e3-42ef-8a3f-15d7334b083c,
  abstract     = {{The development and evaluation of a radioimmunoassay for Nα-triglycyl-lysine8-vasopressin is described. The site of hapten conjugation of the immunogen has been controlled and the use of various radiolabelled tracers has been evaluated with special reference to the site of iodination. The most extensively studied antiserum showed specificity for the N-terminal triglycyl-extension as well as for several aminoacid residues of the vasopressin ring. It crossreacted 27%, 28% and 0.3% with Lys8-vasopressin, arg8-vasopressin and oxytocin respectively, and it was used to quantify triglycyl-lysine8-vasopressin in human plasma after SepPak C18 extraction. The sensitivity of the assay was 5 pg/tube with an intra-assay CV of 5–6% at 17 and 70 pg/tube. The identity of the immunoreactivity was studied by reversed phase chromatography.<br/><br/>}},
  author       = {{Ohlin, Mats and Alkner, Ulf}},
  issn         = {{0197-1522}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{19--36}},
  publisher    = {{Marcel Dekker}},
  series       = {{J Immunoassay}},
  title        = {{Development of an immunoassay for glypressin, an N-terminal extended vasopressin analogue.}},
  url          = {{http://dx.doi.org/10.1080/01971528808053208}},
  doi          = {{10.1080/01971528808053208}},
  volume       = {{9}},
  year         = {{1988}},
}