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Direct electron transfer of dehydrogenases for development of 3rd generation biosensors and enzymatic fuel cells

Bollella, Paolo; Gorton, Lo LU and Antiochia, Riccarda (2018) In Sensors (Switzerland) 18(5).
Abstract

Dehydrogenase based bioelectrocatalysis has been increasingly exploited in recent years in order to develop new bioelectrochemical devices, such as biosensors and biofuel cells, with improved performances. In some cases, dehydrogeases are able to directly exchange electrons with an appropriately designed electrode surface, without the need for an added redox mediator, allowing bioelectrocatalysis based on a direct electron transfer process. In this review we briefly describe the electron transfer mechanism of dehydrogenase enzymes and some of the characteristics required for bioelectrocatalysis reactions via a direct electron transfer mechanism. Special attention is given to cellobiose dehydrogenase and fructose dehydrogenase, which... (More)

Dehydrogenase based bioelectrocatalysis has been increasingly exploited in recent years in order to develop new bioelectrochemical devices, such as biosensors and biofuel cells, with improved performances. In some cases, dehydrogeases are able to directly exchange electrons with an appropriately designed electrode surface, without the need for an added redox mediator, allowing bioelectrocatalysis based on a direct electron transfer process. In this review we briefly describe the electron transfer mechanism of dehydrogenase enzymes and some of the characteristics required for bioelectrocatalysis reactions via a direct electron transfer mechanism. Special attention is given to cellobiose dehydrogenase and fructose dehydrogenase, which showed efficient direct electron transfer reactions. An overview of the most recent biosensors and biofuel cells based on the two dehydrogenases will be presented. The various strategies to prepare modified electrodes in order to improve the electron transfer properties of the device will be carefully investigated and all analytical parameters will be presented, discussed and compared.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Biofuel cells, Biosensors, Cellobiose dehydrogenase, Dehydrogenases, Direct electron transfer, Fructose dehydrogenase
in
Sensors (Switzerland)
volume
18
issue
5
publisher
MDPI AG
external identifiers
  • scopus:85046103243
ISSN
1424-8220
DOI
10.3390/s18051319
language
English
LU publication?
yes
id
aed2000a-5c14-4e8b-bca3-848d04690a6b
date added to LUP
2018-05-07 14:58:19
date last changed
2019-06-19 03:54:52
@article{aed2000a-5c14-4e8b-bca3-848d04690a6b,
  abstract     = {<p>Dehydrogenase based bioelectrocatalysis has been increasingly exploited in recent years in order to develop new bioelectrochemical devices, such as biosensors and biofuel cells, with improved performances. In some cases, dehydrogeases are able to directly exchange electrons with an appropriately designed electrode surface, without the need for an added redox mediator, allowing bioelectrocatalysis based on a direct electron transfer process. In this review we briefly describe the electron transfer mechanism of dehydrogenase enzymes and some of the characteristics required for bioelectrocatalysis reactions via a direct electron transfer mechanism. Special attention is given to cellobiose dehydrogenase and fructose dehydrogenase, which showed efficient direct electron transfer reactions. An overview of the most recent biosensors and biofuel cells based on the two dehydrogenases will be presented. The various strategies to prepare modified electrodes in order to improve the electron transfer properties of the device will be carefully investigated and all analytical parameters will be presented, discussed and compared.</p>},
  articleno    = {1319},
  author       = {Bollella, Paolo and Gorton, Lo and Antiochia, Riccarda},
  issn         = {1424-8220},
  keyword      = {Biofuel cells,Biosensors,Cellobiose dehydrogenase,Dehydrogenases,Direct electron transfer,Fructose dehydrogenase},
  language     = {eng},
  month        = {05},
  number       = {5},
  publisher    = {MDPI AG},
  series       = {Sensors (Switzerland)},
  title        = {Direct electron transfer of dehydrogenases for development of 3rd generation biosensors and enzymatic fuel cells},
  url          = {http://dx.doi.org/10.3390/s18051319},
  volume       = {18},
  year         = {2018},
}