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Evaluation of urine dipsticks for quality control of residual erythrocytes and leukocytes in leukocyte-depleted donor plasma

Ekblom, Kim LU and Petersson, Annika LU (2020) In Scandinavian Journal of Clinical and Laboratory Investigation 80(1). p.39-45
Abstract

Currently used methodologies for quality control of residual leukocytes and erythrocytes in leukocyte-depleted plasma are either expensive or time-consuming. It has been proposed that urine dipsticks could be used as a screening method for residual erythrocytes. The aim was, therefore, to evaluate if urine dipsticks could be used to detect residual erythrocytes and also residual leukocytes in leukocyte-depleted plasma. Dilution series ranging over the decision limits for residual erythrocytes and leukocytes were prepared. Positive, negative and overall agreements, as well as the precision and joint frequency distributions, were calculated for five dipstick analyzers and their corresponding dipsticks. Twenty-four consecutive... (More)

Currently used methodologies for quality control of residual leukocytes and erythrocytes in leukocyte-depleted plasma are either expensive or time-consuming. It has been proposed that urine dipsticks could be used as a screening method for residual erythrocytes. The aim was, therefore, to evaluate if urine dipsticks could be used to detect residual erythrocytes and also residual leukocytes in leukocyte-depleted plasma. Dilution series ranging over the decision limits for residual erythrocytes and leukocytes were prepared. Positive, negative and overall agreements, as well as the precision and joint frequency distributions, were calculated for five dipstick analyzers and their corresponding dipsticks. Twenty-four consecutive leukocyte-depleted donor plasma samples were also tested. None of the dipstick analyzers had both a high positive and a high negative agreement. Accordingly, none of the analyzers were able to discriminate between cell concentrations close to the decision limits. The inconsistency count revealed differences in precision between the dipstick analyzers. In the 24 consecutive donor samples, no significant correlation between the dipstick analyzers and the reference methods were found. In conclusion, urine dipsticks are not suitable for quality control of residual leukocytes and erythrocytes in leukocyte-depleted donor plasma.

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author
and
publishing date
type
Contribution to journal
publication status
published
keywords
plasma, point-of-care testing, practice guideline, Quality control, red blood cell, transfusion
in
Scandinavian Journal of Clinical and Laboratory Investigation
volume
80
issue
1
pages
39 - 45
publisher
Informa Healthcare
external identifiers
  • scopus:85075415267
  • pmid:31766902
ISSN
0036-5513
DOI
10.1080/00365513.2019.1692233
language
English
LU publication?
no
additional info
Publisher Copyright: © 2019, © 2019 Medisinsk Fysiologisk Forenings Forlag (MFFF).
id
af1b35ad-7995-475b-b91a-db7cd773a71d
date added to LUP
2026-02-17 14:13:03
date last changed
2026-02-18 07:44:26
@article{af1b35ad-7995-475b-b91a-db7cd773a71d,
  abstract     = {{<p>Currently used methodologies for quality control of residual leukocytes and erythrocytes in leukocyte-depleted plasma are either expensive or time-consuming. It has been proposed that urine dipsticks could be used as a screening method for residual erythrocytes. The aim was, therefore, to evaluate if urine dipsticks could be used to detect residual erythrocytes and also residual leukocytes in leukocyte-depleted plasma. Dilution series ranging over the decision limits for residual erythrocytes and leukocytes were prepared. Positive, negative and overall agreements, as well as the precision and joint frequency distributions, were calculated for five dipstick analyzers and their corresponding dipsticks. Twenty-four consecutive leukocyte-depleted donor plasma samples were also tested. None of the dipstick analyzers had both a high positive and a high negative agreement. Accordingly, none of the analyzers were able to discriminate between cell concentrations close to the decision limits. The inconsistency count revealed differences in precision between the dipstick analyzers. In the 24 consecutive donor samples, no significant correlation between the dipstick analyzers and the reference methods were found. In conclusion, urine dipsticks are not suitable for quality control of residual leukocytes and erythrocytes in leukocyte-depleted donor plasma.</p>}},
  author       = {{Ekblom, Kim and Petersson, Annika}},
  issn         = {{0036-5513}},
  keywords     = {{plasma; point-of-care testing; practice guideline; Quality control; red blood cell; transfusion}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{1}},
  pages        = {{39--45}},
  publisher    = {{Informa Healthcare}},
  series       = {{Scandinavian Journal of Clinical and Laboratory Investigation}},
  title        = {{Evaluation of urine dipsticks for quality control of residual erythrocytes and leukocytes in leukocyte-depleted donor plasma}},
  url          = {{http://dx.doi.org/10.1080/00365513.2019.1692233}},
  doi          = {{10.1080/00365513.2019.1692233}},
  volume       = {{80}},
  year         = {{2020}},
}